##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940213_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4285620 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17293693794597 34.0 31.0 34.0 30.0 34.0 2 31.7912537742497 34.0 31.0 34.0 30.0 34.0 3 32.20167397016068 34.0 31.0 34.0 30.0 34.0 4 35.70634750631181 37.0 35.0 37.0 35.0 37.0 5 35.77351468398972 37.0 35.0 37.0 35.0 37.0 6 35.853950420242576 37.0 37.0 37.0 35.0 37.0 7 35.83522967505285 37.0 37.0 37.0 35.0 37.0 8 35.837473224410935 37.0 37.0 37.0 35.0 37.0 9 37.58983297632548 39.0 39.0 39.0 35.0 39.0 10-11 37.53227397669416 39.0 38.0 39.0 35.0 39.0 12-13 37.44873355547155 39.0 38.0 39.0 35.0 39.0 14-15 38.888075120985995 41.0 39.0 41.0 36.0 41.0 16-17 38.81622927371069 41.0 39.0 41.0 36.0 41.0 18-19 38.73046502023044 41.0 39.0 41.0 35.0 41.0 20-21 38.64738089237963 41.0 39.0 41.0 35.0 41.0 22-23 38.54308174779845 40.0 39.0 41.0 34.5 41.0 24-25 38.43149905964598 40.0 38.5 41.0 34.0 41.0 26-27 38.27254481265254 40.0 38.0 41.0 34.0 41.0 28-29 38.11199698060024 40.0 38.0 41.0 34.0 41.0 30-31 37.96872984538993 40.0 38.0 41.0 33.5 41.0 32-33 38.03993494523546 40.0 38.0 41.0 34.0 41.0 34-35 38.11909537009814 40.0 38.0 41.0 33.5 41.0 36-37 38.25215161400217 40.0 38.0 41.0 34.0 41.0 38-39 38.17705046177682 40.0 38.0 41.0 34.0 41.0 40-41 38.04706483542638 40.0 38.0 41.0 33.0 41.0 42-43 37.91545225661631 40.0 38.0 41.0 33.0 41.0 44-45 37.77512401939509 40.0 38.0 41.0 33.0 41.0 46-47 37.57627122796701 40.0 38.0 41.0 33.0 41.0 48-49 37.400089485302004 40.0 37.5 41.0 32.5 41.0 50-51 36.47057228592362 39.0 36.0 40.0 31.0 40.5 52-53 36.4694135270976 39.0 36.0 40.5 31.0 41.0 54-55 36.86113292825776 40.0 36.0 41.0 31.0 41.0 56-57 36.739898544434645 39.5 36.0 41.0 31.0 41.0 58-59 36.48309171135098 39.0 35.5 41.0 31.0 41.0 60-61 36.173468132965596 39.0 35.0 41.0 31.0 41.0 62-63 35.82730526738255 38.5 35.0 40.5 30.0 41.0 64-65 35.45039107527032 37.5 35.0 40.0 30.0 41.0 66-67 35.0504119590631 37.0 35.0 39.5 29.0 41.0 68-69 34.61894650015634 36.0 34.5 39.0 29.0 41.0 70-71 34.206707197558345 36.0 34.0 39.0 29.0 40.5 72-73 33.79984062982719 35.0 34.0 37.5 28.0 40.0 74-75 33.384326538517186 35.0 34.0 37.0 27.5 39.0 76-77 32.90231751765205 35.0 33.5 36.5 26.5 39.0 78-79 32.50151179992626 35.0 33.0 36.0 26.0 38.0 80-81 32.18046677026895 35.0 33.0 36.0 26.0 37.0 82-83 31.856079983759642 35.0 33.0 35.0 25.0 37.0 84-85 31.593150349307685 35.0 33.0 35.0 24.5 36.0 86-87 31.321854480798578 35.0 32.0 35.0 24.0 36.0 88-89 31.060227925014352 35.0 32.0 35.0 23.0 36.0 90-91 30.79423852324751 34.5 32.0 35.0 20.0 35.0 92-93 30.529624535073104 34.0 32.0 35.0 19.0 35.0 94-95 30.212507291827087 34.0 31.0 35.0 16.5 35.0 96-97 29.864276814089912 34.0 31.0 35.0 4.5 35.0 98-99 29.453296022512497 34.0 31.0 35.0 2.0 35.0 100-101 27.602309467474953 32.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.13383644504973802 1101 2 0.09856361808098413 1101 3 0.18622579168336273 1101 4 0.22310603303396448 1101 5 0.3078111441074256 1101 6 0.3656047404086138 1101 7 0.3239928406230703 1101 8 0.38480037483805773 1101 9 0.49340568255669837 1101 10-11 0.531827033436933 1101 12-13 0.5432105086493166 1101 14-15 0.5699785048599679 1101 16-17 0.6144788509658454 1101 18-19 0.6733685875946733 1101 20-21 0.7057907681365876 1101 22-23 0.6621199220043295 1101 24-25 0.6006006340588002 1101 26-27 0.7763314849140599 1101 28-29 0.6923307710121378 1101 30-31 0.7405806823010224 1101 32-33 0.6873729305027823 1101 34-35 0.6596399500150767 1101 36-37 0.6583132010884398 1101 38-39 0.7635395267730232 1101 40-41 0.7433163201012789 1101 42-43 0.798399917537175 1101 44-45 0.7535249577365803 1101 46-47 0.8698201391847888 1101 48-49 0.8768379794174379 1101 50-51 0.7861279484711403 1101 52-53 0.9693221027783778 1101 54-55 0.937475273528726 1101 56-57 0.9618845314278346 1101 58-59 0.9489615963791422 1101 60-61 0.9705162935422109 1101 62-63 1.0192182982857432 1101 64-65 1.0159959330698953 1101 66-67 1.0118032443410598 1101 68-69 1.0205897495291723 1101 70-71 0.9897796498908136 1101 72-73 0.9825920523861882 1101 74-75 0.9185171069779301 1101 76-77 1.0585410176140257 1101 78-79 0.976364377954269 1101 80-81 1.0239843618558027 1101 82-83 0.940860898930584 1101 84-85 1.0239307452188164 1101 86-87 0.8887546845960053 1101 88-89 1.0454401102675313 1101 90-91 1.0309848936503236 1101 92-93 0.9672773841282272 1101 94-95 0.9975314442333278 1101 96-97 1.0056018399185653 1101 98-99 0.9448702482781144 1101 100-101 1.08372632029943 1102 1 0.28347746048200406 1102 2 0.7479112832030452 1102 3 0.42472435712478784 1102 4 0.3638006852545246 1102 5 0.273320801672682 1102 6 0.2124354730091298 1102 7 0.23392772539519768 1102 8 0.26001956632124035 1102 9 0.2794907272096765 1102 10-11 0.24405875587300585 1102 12-13 0.255524109657415 1102 14-15 0.3152518856680899 1102 16-17 0.3246622129772092 1102 18-19 0.3100525859101211 1102 20-21 0.31318761427583297 1102 22-23 0.2762155358094702 1102 24-25 0.3709255729085612 1102 26-27 0.35478347565562274 1102 28-29 0.2946025277173163 1102 30-31 0.39680360159979244 1102 32-33 0.3571095919331668 1102 34-35 0.3110308016901939 1102 36-37 0.44625607628940855 1102 38-39 0.45507387372530417 1102 40-41 0.4653310947077145 1102 42-43 0.3076321576889143 1102 44-45 0.38807025818577046 1102 46-47 0.43958511729685057 1102 48-49 0.43603766524024223 1102 50-51 0.41777629141966344 1102 52-53 0.5716070815999998 1102 54-55 0.5865843656801459 1102 56-57 0.513131812270835 1102 58-59 0.5076406921637684 1102 60-61 0.537107963707399 1102 62-63 0.6364582299314989 1102 64-65 0.6374324495004728 1102 66-67 0.47454422357265 1102 68-69 0.4775998035936695 1102 70-71 0.4240226976855652 1102 72-73 0.44335521652241994 1102 74-75 0.5101003839735441 1102 76-77 0.5904937813155868 1102 78-79 0.562727875452083 1102 80-81 0.555946569926256 1102 82-83 0.45760083747483904 1102 84-85 0.31999881628777516 1102 86-87 0.4007480475862941 1102 88-89 0.31736517158201494 1102 90-91 0.2952056494494961 1102 92-93 0.3325998625145843 1102 94-95 0.31463179631939653 1102 96-97 0.061833365911770244 1102 98-99 0.1477624753818425 1102 100-101 0.3390541762273038 1103 1 0.21396102841578823 1103 2 0.6592600500866013 1103 3 0.3371242156637919 1103 4 0.21290117341133907 1103 5 0.12364629857594167 1103 6 0.20975588495610964 1103 7 0.15082381505597198 1103 8 0.0825313566351582 1103 9 0.13477608657804296 1103 10-11 0.1088831198487199 1103 12-13 0.16700856349324766 1103 14-15 0.20066366948067582 1103 16-17 0.1512326590119386 1103 18-19 0.0809376253216314 1103 20-21 0.2043708841852947 1103 22-23 0.1784986371648145 1103 24-25 0.05480849268180776 1103 26-27 0.15568768009813283 1103 28-29 0.03140712180999827 1103 30-31 0.12405704851005339 1103 32-33 0.20533714277829773 1103 34-35 0.17597940571146609 1103 36-37 0.17639531368003958 1103 38-39 0.10888263904549689 1103 40-41 0.0788941486968966 1103 42-43 0.14794208553419708 1103 44-45 0.2434835572905385 1103 46-47 0.1952567117409103 1103 48-49 0.20506599740948417 1103 50-51 0.0727345538475177 1103 52-53 0.1897972788485376 1103 54-55 0.32676642968011294 1103 56-57 0.2954876008514091 1103 58-59 0.32746404046366706 1103 60-61 0.2885060041487577 1103 62-63 0.21322820630154382 1103 64-65 0.35977646990202317 1103 66-67 0.311174824683242 1103 68-69 0.3188484094110251 1103 70-71 0.2501938933219279 1103 72-73 0.2384388270058153 1103 74-75 0.3214304437023685 1103 76-77 0.38993401492861324 1103 78-79 0.3968883479298029 1103 80-81 0.36681470586136555 1103 82-83 0.272138718893995 1103 84-85 0.1424460043077076 1103 86-87 0.2841019178929649 1103 88-89 0.2921189871965062 1103 90-91 0.2825917274306313 1103 92-93 0.16895975376770522 1103 94-95 0.2363637929913196 1103 96-97 0.022835240927737033 1103 98-99 -0.023424401884295065 1103 100-101 0.15782368181959683 1104 1 0.17989377083321045 1104 2 0.6504100420123109 1104 3 0.35178273262096127 1104 4 0.13250472989123097 1104 5 0.10263448580670342 1104 6 0.06195944996595415 1104 7 0.07135801123492058 1104 8 -0.10773591885789102 1104 9 0.07262523594614123 1104 10-11 0.01201906821601284 1104 12-13 0.06885964218594864 1104 14-15 0.056504007723674476 1104 16-17 0.10431451751605891 1104 18-19 0.07573836404954903 1104 20-21 0.02366833444637706 1104 22-23 -0.0863542642888433 1104 24-25 -0.011753297286212216 1104 26-27 -0.008448231451843924 1104 28-29 -0.08095398243246166 1104 30-31 -0.07158746899658297 1104 32-33 -0.030731330383268585 1104 34-35 0.01034215567582919 1104 36-37 -0.03262084620578065 1104 38-39 -0.0024367052465947836 1104 40-41 -0.08158566964184644 1104 42-43 -0.13544083169257704 1104 44-45 -0.08612046335486667 1104 46-47 -0.139141610069899 1104 48-49 -0.1380237932214854 1104 50-51 -0.20282662772346072 1104 52-53 -0.09429273134973215 1104 54-55 0.0028191528067225136 1104 56-57 -0.008010785444390933 1104 58-59 0.02401790316520902 1104 60-61 0.0715655547612073 1104 62-63 0.013693416792712299 1104 64-65 -0.004349222716150791 1104 66-67 5.811764358796268E-4 1104 68-69 0.004360661618633799 1104 70-71 -0.00914746796859589 1104 72-73 -0.11786163656429949 1104 74-75 -0.1413194842122394 1104 76-77 0.049328542611654314 1104 78-79 0.04190367815014895 1104 80-81 -0.09953104665360257 1104 82-83 -0.19269600476895832 1104 84-85 -0.08609123154779041 1104 86-87 0.03858430469520968 1104 88-89 -0.1079049562102945 1104 90-91 -0.1916527065019089 1104 92-93 -0.2523795933847204 1104 94-95 -0.3726401916630273 1104 96-97 -0.4092829224884156 1104 98-99 -0.4428474563047864 1104 100-101 -0.27799164494988915 1105 1 -0.060207429366940346 1105 2 0.45995254388876816 1105 3 0.08126352279565197 1105 4 0.14457169476826692 1105 5 -0.025614024981116756 1105 6 0.039080174574834814 1105 7 0.03571902055100651 1105 8 -0.03553695311988747 1105 9 0.02437048408071263 1105 10-11 0.0021276270927828023 1105 12-13 -0.15423082722403336 1105 14-15 -0.3455420608113613 1105 16-17 -0.21738003393579675 1105 18-19 -0.3457616308913529 1105 20-21 -0.18465309852711442 1105 22-23 -0.16673791809254368 1105 24-25 -0.1634993847698425 1105 26-27 -0.14524608722546617 1105 28-29 0.022669981833800534 1105 30-31 -0.13923331106834524 1105 32-33 0.07649740810040129 1105 34-35 -0.06845006933042441 1105 36-37 -0.09742445692443624 1105 38-39 -0.300964015595099 1105 40-41 -0.3140694897516383 1105 42-43 -0.16910568490537514 1105 44-45 -0.1707036219196354 1105 46-47 -0.1904737260021676 1105 48-49 -0.38471325108938714 1105 50-51 -0.40076542790962577 1105 52-53 -0.12708121216934387 1105 54-55 -0.23875131066112232 1105 56-57 -0.07303728357896233 1105 58-59 -0.15278988222267031 1105 60-61 -0.09380150062755632 1105 62-63 -0.05495268789182717 1105 64-65 0.09810448200958888 1105 66-67 -0.0915770498287074 1105 68-69 -0.09393889768862351 1105 70-71 -0.14793383362859203 1105 72-73 -0.3239363160939348 1105 74-75 -0.3629276402014696 1105 76-77 -0.2899906717208438 1105 78-79 -0.13110145360695924 1105 80-81 -0.10472529501997485 1105 82-83 -0.23841195571234053 1105 84-85 -0.32106711348518147 1105 86-87 -0.1813863732780625 1105 88-89 -0.2122427121082744 1105 90-91 -0.41932619420187933 1105 92-93 -0.422508172461054 1105 94-95 -0.41993590100265976 1105 96-97 -0.4887465692461781 1105 98-99 -0.5732954640350769 1105 100-101 -0.4781742091175438 1106 1 -0.034778079710527265 1106 2 0.5390079432954664 1106 3 0.10934904519868383 1106 4 0.057514356637611286 1106 5 -0.005935517722434724 1106 6 0.03917814081802362 1106 7 0.038197846407108216 1106 8 -0.06750272248294209 1106 9 -0.03315809295232697 1106 10-11 -0.08593383704015167 1106 12-13 -0.061231752473212 1106 14-15 -0.053978482622881074 1106 16-17 -0.08833996204761263 1106 18-19 -0.03686614486235129 1106 20-21 -0.1117196656376862 1106 22-23 -0.17279693430096899 1106 24-25 -0.24261941390668085 1106 26-27 -0.41230410944040585 1106 28-29 -0.31723459120803454 1106 30-31 -0.23864534006903426 1106 32-33 -0.24482428415617363 1106 34-35 -0.32643476599558596 1106 36-37 -0.3321320383248647 1106 38-39 -0.25583799293948317 1106 40-41 -0.3848852003573384 1106 42-43 -0.32469614816591275 1106 44-45 -0.2242134557151374 1106 46-47 -0.19512362375899528 1106 48-49 -0.2009513910160834 1106 50-51 -0.2861091237203013 1106 52-53 -0.14316203510370684 1106 54-55 -0.14001244970287985 1106 56-57 -0.19425866959866056 1106 58-59 -0.18907789424464028 1106 60-61 -0.21184516961227473 1106 62-63 -0.21590876229153366 1106 64-65 -0.3029526353331491 1106 66-67 -0.19386948231149148 1106 68-69 -0.4236713557971328 1106 70-71 -0.24265851377720793 1106 72-73 -0.336632965133397 1106 74-75 -0.1295166280343949 1106 76-77 -0.13621325572773912 1106 78-79 -0.11561355036670307 1106 80-81 -0.21578114383981273 1106 82-83 -0.21509316280872426 1106 84-85 -0.3034822288144028 1106 86-87 -0.25243376005371587 1106 88-89 -0.2971412661041306 1106 90-91 -0.23503889918084653 1106 92-93 -0.26344127909490567 1106 94-95 -0.3503530535818946 1106 96-97 -0.3640385926219345 1106 98-99 -0.4601847872596352 1106 100-101 -0.2991626202148048 1107 1 0.07166839408496628 1107 2 0.5120564635198619 1107 3 0.18269221186021412 1107 4 0.034527640091056355 1107 5 0.023268139050500736 1107 6 -0.003739324766399932 1107 7 -0.011485360213157492 1107 8 -0.011903883291161321 1107 9 -0.12877098495749095 1107 10-11 -0.08071163509429624 1107 12-13 -0.051319924720736765 1107 14-15 -0.08950512899318142 1107 16-17 -0.06516494829680397 1107 18-19 -0.08959088813773519 1107 20-21 -0.09101117659036362 1107 22-23 -0.08454712428490296 1107 24-25 -0.08444285479618685 1107 26-27 -0.02038504473534175 1107 28-29 -0.12174289695407481 1107 30-31 -0.2475841427547678 1107 32-33 -0.19951852569406725 1107 34-35 -0.2177624869852366 1107 36-37 -0.21810133439478818 1107 38-39 -0.16158963318402897 1107 40-41 -0.12301835338800515 1107 42-43 -0.12014632225715616 1107 44-45 -0.16288082877075283 1107 46-47 -0.2545846038019093 1107 48-49 -0.13850627645170732 1107 50-51 -0.2726517503733987 1107 52-53 -0.17305556164233593 1107 54-55 -0.19059481362555886 1107 56-57 -0.20290079642481373 1107 58-59 -0.1543390011291237 1107 60-61 -0.24521372065390779 1107 62-63 -0.20217559632397553 1107 64-65 -0.2091665983593245 1107 66-67 -0.22374108144925486 1107 68-69 -0.07295631369652256 1107 70-71 -0.13144420912266241 1107 72-73 -0.15171134903200567 1107 74-75 -0.13660188262571182 1107 76-77 0.022071869451750104 1107 78-79 -0.08532782053713106 1107 80-81 -0.1340865022004678 1107 82-83 -0.10151822218137596 1107 84-85 -0.25465571691074373 1107 86-87 -0.23286755740191012 1107 88-89 -0.2644804426748095 1107 90-91 -0.1791818842545787 1107 92-93 -0.1285667198918894 1107 94-95 -0.029218262662741523 1107 96-97 -0.034381244806130695 1107 98-99 -0.0919209169336277 1107 100-101 -0.07393137314916842 1108 1 0.0995950681871065 1108 2 0.2929439043085367 1108 3 0.13542340445166445 1108 4 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-1.299232958955276 2316 88-89 -1.3439332361330507 2316 90-91 -1.2998687136644627 2316 92-93 -1.3994756197237415 2316 94-95 -1.3760637366798747 2316 96-97 -1.1077012664251527 2316 98-99 -1.135316354919052 2316 100-101 -1.525789258087915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25422.0 3 9385.0 4 7451.0 5 5801.0 6 5132.0 7 4828.0 8 5198.0 9 5928.0 10 6577.0 11 7679.0 12 8366.0 13 9207.0 14 9991.0 15 11157.0 16 12597.0 17 15181.0 18 14614.0 19 12988.0 20 13419.0 21 14424.0 22 15595.0 23 17216.0 24 19188.0 25 22639.0 26 26279.0 27 31815.0 28 38756.0 29 46738.0 30 57435.0 31 72181.0 32 94142.0 33 125093.0 34 182088.0 35 292266.0 36 559412.0 37 1140777.0 38 1182898.0 39 155750.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45971401369315 17.87971097471227 13.558172792905124 25.102402218689452 2 16.792089524820142 19.711856165708966 37.65176383699409 25.844290472476807 3 18.934182142241937 23.024579637455833 28.78237593358788 29.258862286714354 4 13.88405621205791 16.12945764840156 35.14693697499586 34.83954916454467 5 15.64731120153169 35.63516020710373 33.52091985315175 15.196608738212824 6 34.44221771274041 33.838199232267726 17.250028359318872 14.469554695672999 7 30.332722812060826 30.460234385084018 20.601673498949776 18.605369303905384 8 27.38353734094327 33.49169860230104 19.864606447718806 19.26015760903688 9 26.904007807268222 15.873463807705173 19.272991328862503 37.949537056164104 10-11 25.972674173960066 25.02908529687774 27.134592400925033 21.863648128237163 12-13 26.500408221949506 22.164934738170782 27.261920711113767 24.07273632876595 14-15 23.84234728618666 23.754672977629806 24.357715379996883 28.04526435618665 16-17 24.42939822865901 26.73196177578545 25.00996735283449 23.828672642721056 18-19 24.42952708358112 26.019197905759405 26.139618140514088 23.41165687014539 20-21 24.80984866214441 25.454197406287076 25.917251208234145 23.81870272333437 22-23 24.77632551184078 25.08213273284538 25.836275141533 24.30526661378084 24-25 24.741988595059258 25.334837811567603 25.646682543812872 24.276491049560267 26-27 24.525387083722862 25.61339578844601 25.59985884232737 24.26135828550376 28-29 24.479902267304244 25.554093641983044 25.776134895021148 24.189869195691564 30-31 24.546548460727745 25.367751961252377 25.597478412307222 24.488221165712652 32-33 24.490748640171834 25.37513004235815 25.582637614984055 24.55148370248596 34-35 24.81764969788019 25.407965617677803 25.365622626376556 24.408762058065452 36-37 24.756475649899134 25.238593467075997 25.415603414621906 24.58932746840296 38-39 24.531269837713502 25.357078843008534 25.72973612573656 24.3819151935414 40-41 24.73570691268287 25.161829121237005 25.66330861175737 24.439155354322754 42-43 24.741555881841915 25.183944486251264 25.462829473732363 24.611670158174466 44-45 24.664978689452997 25.348155511270498 25.343312602962857 24.643553196313647 46-47 24.685428707422997 25.33531302631055 25.28903357990422 24.69022468636223 48-49 24.825657599928302 25.33394621571966 25.27363821548067 24.566757968871364 50-51 24.53942368050044 25.392920447547528 25.43896961886667 24.62868625308536 52-53 24.873918589466044 25.269767653556503 25.16025240789994 24.69606134907751 54-55 24.702980523140806 25.159132790189332 25.29099751068724 24.846889175982625 56-57 24.607454555830973 25.51249239791321 25.412204957044928 24.46784808921089 58-59 24.70488623526007 25.134655064561606 25.43207960264035 24.728379097537974 60-61 24.79812655750156 25.120127404546665 25.542576270529725 24.53916976742205 62-63 24.69403058672902 25.26636317641203 25.449671134598766 24.58993510226019 64-65 24.625334040725132 25.145444406917093 25.40399408722533 24.82522746513244 66-67 24.536615514169767 25.38053952039779 25.29464869836894 24.788196267063505 68-69 24.37196124632502 25.212749954866894 25.62575729974815 24.789531499059937 70-71 24.35151675432932 25.195853581992054 25.52120540651005 24.931424257168576 72-73 24.574289338671036 25.134820482654703 25.42233249149419 24.868557687180072 74-75 24.459562786177315 25.256961498837438 25.35665821915027 24.926817495834978 76-77 24.628714677017925 25.161120736173086 25.39066708391048 24.81949750289851 78-79 24.451730840688715 25.22144332264648 25.470580189674518 24.856245646990285 80-81 24.3228068947006 25.325677741600437 25.587998459545563 24.76351690415339 82-83 24.468364694234136 25.185859376130548 25.439032382897857 24.90674354673746 84-85 24.43864905596786 25.107020962594657 25.42423854127061 25.030091440166874 86-87 24.5200183443629 24.98154477739956 25.68089443939417 24.817542438843372 88-89 24.37857833628055 24.94001412750299 25.625196855539457 25.056210680677005 90-91 24.282531030486982 25.129276920949494 25.716132819178018 24.872059229385503 92-93 24.393557989208226 24.927591104023243 25.738538059254847 24.94031284751368 94-95 24.31072971395213 24.846818447168708 25.69415061295972 25.14830122591944 96-97 24.491154707581593 24.819317315750368 25.632040552790052 25.057487423877987 98-99 24.13451216907715 24.822540008264788 25.683278330106468 25.35966949255159 100-101 24.023346389827726 24.70078169654247 26.01111738409272 25.264754529537083 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 307.0 1 194.5 2 130.0 3 161.0 4 119.5 5 125.0 6 237.5 7 458.5 8 726.0 9 882.0 10 851.0 11 810.5 12 868.5 13 936.5 14 999.5 15 1040.0 16 1071.0 17 1124.0 18 1201.0 19 1302.5 20 1435.0 21 1626.0 22 1858.5 23 2235.0 24 2746.0 25 3495.0 26 4689.0 27 6445.0 28 8746.5 29 11873.5 30 16259.0 31 21473.0 32 26984.5 33 33071.0 34 40063.0 35 46923.0 36 54823.0 37 63942.5 38 74141.5 39 82712.0 40 90402.5 41 102850.0 42 115564.0 43 128365.5 44 140914.0 45 151036.0 46 160230.0 47 169763.5 48 184340.0 49 195903.5 50 200821.0 51 203289.5 52 202346.0 53 202856.0 54 201522.0 55 191697.0 56 178819.0 57 165018.5 58 149052.5 59 131185.0 60 110792.0 61 89797.5 62 71702.0 63 56581.5 64 43564.5 65 32836.5 66 24195.0 67 18690.5 68 14130.5 69 10168.0 70 7502.0 71 5458.5 72 4101.5 73 3138.5 74 2412.5 75 1820.0 76 1252.0 77 751.5 78 457.5 79 307.0 80 205.0 81 155.0 82 116.5 83 90.0 84 94.5 85 76.5 86 44.0 87 38.0 88 40.5 89 36.5 90 27.5 91 21.5 92 20.5 93 17.5 94 13.5 95 13.5 96 16.0 97 13.0 98 5.0 99 4.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030077328367890763 2 0.03684414390449923 3 0.03131402224182265 4 0.026367246746095082 5 0.02793061447351842 6 0.030660674534839765 7 0.030404002221382204 8 0.02849062679378946 9 0.03283072227589007 10-11 0.01887708196246984 12-13 0.027673942160060857 14-15 0.023625519761434753 16-17 0.038127505471787046 18-19 0.0436692940578026 20-21 0.046492689505835794 22-23 0.036132461580821444 24-25 0.014816992640504758 26-27 0.02448887208851928 28-29 0.02989065759446708 30-31 0.02745227061662023 32-33 0.0335774053695848 34-35 0.03635413312426207 36-37 0.03260905073244945 38-39 0.019880437369622132 40-41 0.016310358827894212 42-43 0.016322025751233194 44-45 0.023438848988011067 46-47 0.018212067332147974 48-49 0.022120486650706313 50-51 0.024955549022078485 52-53 0.029983992981178917 54-55 0.03045066991473812 56-57 0.03347240305953397 58-59 0.031267354548466735 60-61 0.030672341458178747 62-63 0.03669247390109249 64-65 0.031197353008432856 66-67 0.025923903659213838 68-69 0.025002216715434403 70-71 0.034732430780143826 72-73 0.01762872116519897 74-75 0.026273911359383235 76-77 0.04558266948539535 78-79 0.028653963720535185 80-81 0.02730060061321349 82-83 0.027953948320196376 84-85 0.02751060523331513 86-87 0.02158380817711323 88-89 0.024955549022078485 90-91 0.02152547356041833 92-93 0.03770749623158376 94-95 0.07280160163523597 96-97 0.08392017957728404 98-99 0.08396684727063995 100-101 0.08371017495718239 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4285620.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.38467594186865 #Duplication Level Percentage of deduplicated Percentage of total 1 76.40243288838892 39.25914255140232 2 11.836281747730059 12.164070039275273 3 4.280017603050447 6.597819526747218 4 2.1912109346003685 4.503786551788762 5 1.2366819439126673 3.1773250465556293 6 0.8094360186350935 2.495556450794438 7 0.5814040822498094 2.0912682250380206 8 0.4232740051044211 1.7399838069526028 9 0.315413144790959 1.458666200960021 >10 1.7593225154162069 16.12893807595813 >50 0.10717483801128803 3.776524973289961 >100 0.053797971939906965 5.0161105386312395 >500 0.0027325433166332336 0.9759723963607365 >1k 8.197628530538996E-4 0.6148356162457275 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 7.000154003388074E-5 14-15 0.0 1.1666923338980124E-5 0.0 2.3333846677960247E-5 9.333538671184099E-5 16-17 2.3333846677960247E-5 2.3333846677960247E-5 0.0 2.3333846677960247E-5 9.333538671184099E-5 18-19 3.500077001694037E-5 2.3333846677960247E-5 0.0 2.3333846677960247E-5 9.333538671184099E-5 20-21 4.6667693355920495E-5 2.3333846677960247E-5 0.0 4.6667693355920495E-5 9.333538671184099E-5 22-23 7.000154003388074E-5 2.3333846677960247E-5 0.0 5.833461669490062E-5 9.333538671184099E-5 24-25 9.333538671184099E-5 4.6667693355920495E-5 0.0 9.333538671184099E-5 1.2833615672878137E-4 26-27 9.333538671184099E-5 7.000154003388074E-5 0.0 1.983376967626621E-4 1.4000308006776148E-4 28-29 9.333538671184099E-5 7.000154003388074E-5 0.0 6.766815536608471E-4 1.4000308006776148E-4 30-31 9.333538671184099E-5 7.000154003388074E-5 0.0 0.0025083885178807264 1.4000308006776148E-4 32-33 1.0500231005082111E-4 7.000154003388074E-5 0.0 0.007420163243591359 1.4000308006776148E-4 34-35 1.1666923338980124E-4 7.000154003388074E-5 0.0 0.013976974160098188 1.4000308006776148E-4 36-37 1.1666923338980124E-4 7.000154003388074E-5 0.0 0.025083885178807268 1.4000308006776148E-4 38-39 1.4000308006776148E-4 7.000154003388074E-5 0.0 0.04340095482100606 1.6333692674572173E-4 40-41 1.516700034067416E-4 7.000154003388074E-5 0.0 0.06422641298108558 1.8667077342368198E-4 42-43 1.6333692674572173E-4 7.000154003388074E-5 0.0 0.08562355038477514 1.983376967626621E-4 44-45 1.8667077342368198E-4 7.000154003388074E-5 0.0 0.11245747406442942 2.1000462010164223E-4 46-47 2.2167154344062236E-4 7.000154003388074E-5 0.0 0.14465818248001455 2.1000462010164223E-4 48-49 2.3333846677960247E-4 7.000154003388074E-5 0.0 0.17907560633000594 2.2167154344062236E-4 50-51 2.3333846677960247E-4 7.000154003388074E-5 0.0 0.2151380663707935 3.0334000681348324E-4 52-53 2.3333846677960247E-4 7.000154003388074E-5 0.0 0.25289223029573316 3.3834077683042357E-4 54-55 2.3333846677960247E-4 1.0500231005082111E-4 0.0 0.2979032205375185 3.5000770016940374E-4 56-57 2.5667231345756275E-4 1.1666923338980124E-4 0.0 0.3529477648508267 3.5000770016940374E-4 58-59 2.5667231345756275E-4 1.1666923338980124E-4 0.0 0.411504053089168 3.5000770016940374E-4 60-61 2.5667231345756275E-4 1.1666923338980124E-4 0.0 0.47433043526957597 3.5000770016940374E-4 62-63 2.5667231345756275E-4 1.1666923338980124E-4 0.0 0.5415085798554236 3.7334154684736396E-4 64-65 2.5667231345756275E-4 1.1666923338980124E-4 0.0 0.6213220024173866 3.7334154684736396E-4 66-67 2.8000616013552297E-4 1.1666923338980124E-4 0.0 0.7183791376743622 3.7334154684736396E-4 68-69 2.8000616013552297E-4 1.1666923338980124E-4 0.0 0.8238714585054205 3.7334154684736396E-4 70-71 2.8000616013552297E-4 1.1666923338980124E-4 0.0 0.9421623942393399 4.0834231686430434E-4 72-73 2.9167308347450313E-4 1.1666923338980124E-4 0.0 1.0683868378437658 4.900107802371652E-4 74-75 3.0334000681348324E-4 1.1666923338980124E-4 0.0 1.2108049710426965 4.900107802371652E-4 76-77 3.0334000681348324E-4 1.1666923338980124E-4 0.0 1.3771519640098748 4.900107802371652E-4 78-79 3.0334000681348324E-4 1.1666923338980124E-4 0.0 1.5675561529020303 4.900107802371652E-4 80-81 3.9667539352532423E-4 1.4000308006776148E-4 0.0 1.772182321344403 4.900107802371652E-4 82-83 3.9667539352532423E-4 1.4000308006776148E-4 0.0 1.9979139541069904 4.900107802371652E-4 84-85 4.0834231686430434E-4 1.4000308006776148E-4 0.0 2.249114480518571 5.366784735930857E-4 86-87 4.2000924020328445E-4 1.4000308006776148E-4 0.0 2.5292489768108233 5.483453969320658E-4 88-89 4.2000924020328445E-4 1.4000308006776148E-4 0.0 2.849319351692404 5.600123202710459E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3510 0.0 41.414436 1 GTATCAA 5765 0.0 38.646576 1 ATCAACG 6575 0.0 33.086933 3 TCAACGC 6725 0.0 32.48982 4 CAACGCA 6765 0.0 32.016487 5 AACGCAG 7205 0.0 30.194187 6 TATCAAC 7175 0.0 30.188736 2 ACGCAGA 8445 0.0 25.704456 7 GTACATG 11080 0.0 24.867132 1 CGCAGAG 9000 0.0 24.013792 8 TACATGG 11570 0.0 23.073046 2 ACATGGG 11870 0.0 22.409082 3 CATGGGG 10085 0.0 20.346493 4 GTATAAG 970 0.0 20.079348 1 GCAGAGT 11035 0.0 19.15466 9 GCTTATA 2085 0.0 18.455101 1 CAGAGTA 9830 0.0 17.637777 10-11 AGAGTAC 9435 0.0 17.218239 10-11 ATGGGGA 6405 0.0 15.943953 5 GAGTACA 8955 0.0 15.755361 1 >>END_MODULE