FastQCFastQC Report
Fri 27 May 2016
SRR940213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940213_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4285620
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33600.040.581041
GTATCAA53800.039.2085951
TCAACGC64250.032.3070764
ATCAACG64650.032.1802863
CAACGCA66400.031.3325255
TATCAAC71050.029.6165222
AACGCAG70250.029.6153686
GTACATG111400.025.7591951
ACGCAGA83700.024.7996377
TACATGG120950.023.4456832
CGCAGAG89300.023.2444538
ACATGGG120400.022.250353
CATGGGG101750.020.0735134
GCAGAGT111750.018.4470319
GCTTATA19800.017.5161341
AGAGTAC97200.016.68807410-11
GAGTACA85950.016.5827271
CAGAGTA101300.016.4580910-11
CTATAGT8450.016.3016154
AGTACAT83800.016.2112462
Produced by FastQC (version 0.11.5)