FastQCFastQC Report
Fri 27 May 2016
SRR940212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4327106
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33900.040.4904671
GTATCAA55650.039.1743851
TCAACGC67750.031.3384864
CAACGCA67300.031.337025
ATCAACG67650.031.1046813
TATCAAC71750.029.6579322
AACGCAG71600.029.387696
GTACATG111950.026.3888781
ACGCAGA84350.025.0018777
TACATGG117400.024.154112
CGCAGAG89900.023.5112158
ACATGGG120450.022.9906673
CATGGGG103900.020.0234224
GTCCTAA11300.018.9142341
GCAGAGT112400.018.4287289
AGAGTAC96250.017.5225710-11
CAGAGTA100500.016.78156510-11
ATGGGGA64550.016.1888585
GAGTACA86400.016.1067731
CCTATAA19300.015.7498831
Produced by FastQC (version 0.11.5)