##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940212_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4327106 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.370228277282784 34.0 31.0 34.0 31.0 34.0 2 32.38370772520941 34.0 31.0 34.0 31.0 34.0 3 32.48990410680949 34.0 31.0 34.0 31.0 34.0 4 35.871958995226834 37.0 37.0 37.0 35.0 37.0 5 35.87184644887368 37.0 37.0 37.0 35.0 37.0 6 35.94563040517149 37.0 37.0 37.0 35.0 37.0 7 35.903470356399865 37.0 37.0 37.0 35.0 37.0 8 35.89262407715457 37.0 37.0 37.0 35.0 37.0 9 37.652217440478694 39.0 39.0 39.0 35.0 39.0 10-11 37.58427133516027 39.0 38.0 39.0 35.0 39.0 12-13 37.52519131724529 39.0 38.0 39.0 35.0 39.0 14-15 38.97919960823701 41.0 39.0 41.0 36.0 41.0 16-17 38.890147364081216 41.0 39.0 41.0 36.0 41.0 18-19 38.83808034746549 41.0 39.0 41.0 36.0 41.0 20-21 38.763714477990604 41.0 39.0 41.0 35.0 41.0 22-23 38.654263149550765 40.0 39.0 41.0 35.0 41.0 24-25 38.535092854207875 40.0 38.5 41.0 35.0 41.0 26-27 38.37602903649691 40.0 38.0 41.0 34.0 41.0 28-29 38.21766614915373 40.0 38.0 41.0 34.0 41.0 30-31 38.18896544249205 40.0 38.0 41.0 34.0 41.0 32-33 38.21084403756229 40.0 38.0 41.0 34.0 41.0 34-35 38.2425295335959 40.0 38.0 41.0 34.0 41.0 36-37 38.3886463608703 40.5 39.0 41.0 34.5 41.0 38-39 38.31369649830626 40.5 38.0 41.0 34.0 41.0 40-41 38.19843158452786 40.0 38.0 41.0 34.0 41.0 42-43 38.08262127158429 40.0 38.0 41.0 33.5 41.0 44-45 37.930275454310575 40.0 38.0 41.0 33.0 41.0 46-47 37.757125894304416 40.0 38.0 41.0 33.0 41.0 48-49 37.575272017833626 40.0 38.0 41.0 33.0 41.0 50-51 36.622669978502955 39.0 36.0 40.0 31.5 40.5 52-53 36.70302368372765 39.0 36.5 40.5 31.5 41.0 54-55 36.960307766900094 40.0 36.0 41.0 32.0 41.0 56-57 36.88465720506962 40.0 36.0 41.0 32.0 41.0 58-59 36.64539440448189 39.0 35.5 41.0 31.0 41.0 60-61 36.33520267356519 39.0 35.0 41.0 31.0 41.0 62-63 35.980766359779494 38.5 35.0 40.5 31.0 41.0 64-65 35.58969424368158 37.5 35.0 40.0 30.0 41.0 66-67 35.19684819368881 37.0 35.0 40.0 30.0 41.0 68-69 34.788004153353306 36.5 35.0 39.0 29.0 41.0 70-71 34.37683592682962 36.0 34.0 39.0 29.0 40.5 72-73 33.96164156829067 35.0 34.0 37.5 29.0 40.0 74-75 33.54413804515073 35.0 34.0 37.0 28.5 39.0 76-77 33.12690375507325 35.0 34.0 37.0 27.5 39.0 78-79 32.742359443008795 35.0 33.5 36.0 26.5 38.5 80-81 32.36417134223197 35.0 33.0 36.0 26.0 37.0 82-83 32.03862743367045 35.0 33.0 35.0 26.0 37.0 84-85 31.73182341731402 35.0 33.0 35.0 25.0 36.0 86-87 31.472718717775805 35.0 32.5 35.0 24.5 36.0 88-89 31.195067904507077 35.0 32.0 35.0 24.0 36.0 90-91 30.942436700187145 34.5 32.0 35.0 23.0 35.0 92-93 30.65352778508315 34.0 32.0 35.0 20.0 35.0 94-95 30.335576826636554 34.0 31.0 35.0 18.5 35.0 96-97 29.99442132917474 34.0 31.0 35.0 11.0 35.0 98-99 29.563561650673684 34.0 31.0 35.0 2.0 35.0 100-101 27.588075725438664 32.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1349867344145821 1101 2 0.24719932924440968 1101 3 0.20198668091931182 1101 4 0.19278834173527315 1101 5 0.14261903708506196 1101 6 0.07680594329945478 1101 7 0.14095336024124094 1101 8 0.16151504681290163 1101 9 0.1975986633370539 1101 10-11 0.1806897073339755 1101 12-13 0.14097258663508683 1101 14-15 0.24312725492669074 1101 16-17 0.186091990014134 1101 18-19 0.18588360701479445 1101 20-21 0.08136943756575477 1101 22-23 0.18874222620028291 1101 24-25 0.18786101022156032 1101 26-27 0.2328266616227097 1101 28-29 0.22051635779718737 1101 30-31 0.23806203873975562 1101 32-33 0.1407757966569605 1101 34-35 0.1409967823709266 1101 36-37 0.15024827651959782 1101 38-39 0.19857578555694033 1101 40-41 0.16357992356410733 1101 42-43 0.194215085971976 1101 44-45 0.21301227289770708 1101 46-47 0.1717637711555824 1101 48-49 0.23677409834507301 1101 50-51 0.18026128860137192 1101 52-53 0.20681270349821546 1101 54-55 0.4227959528084142 1101 56-57 0.32881877540183524 1101 58-59 0.24845332369675788 1101 60-61 0.19339664742158647 1101 62-63 0.1653175574888337 1101 64-65 0.30485698992536925 1101 66-67 0.301250652289788 1101 68-69 0.251360642280666 1101 70-71 0.300877268888911 1101 72-73 0.19138186529171008 1101 74-75 0.2689414122650149 1101 76-77 0.2963538878055729 1101 78-79 0.3511443391978659 1101 80-81 0.2596591596130864 1101 82-83 0.18553246602434115 1101 84-85 0.2999131123558634 1101 86-87 0.38056066308947223 1101 88-89 0.3450510848758519 1101 90-91 0.3326141143052297 1101 92-93 0.36745545716899386 1101 94-95 0.315265836658984 1101 96-97 0.3355850788468011 1101 98-99 0.16837299639989567 1101 100-101 0.568729512700525 1102 1 0.22880277059472576 1102 2 0.2244521898080052 1102 3 0.22559569043594507 1102 4 0.24045189130310263 1102 5 0.2259008671294822 1102 6 0.24703748794063785 1102 7 0.23367044154464622 1102 8 0.28297231249297283 1102 9 0.28081315786503325 1102 10-11 0.30764791062124175 1102 12-13 0.32305380234410563 1102 14-15 0.44252151737649825 1102 16-17 0.3840062202655332 1102 18-19 0.39876033481777284 1102 20-21 0.39701423768782007 1102 22-23 0.4242119208637689 1102 24-25 0.5068481172486017 1102 26-27 0.38737516349332424 1102 28-29 0.44328597920110013 1102 30-31 0.39817418365069557 1102 32-33 0.3655377030542155 1102 34-35 0.4428739530674406 1102 36-37 0.46372064767786725 1102 38-39 0.5045635810252449 1102 40-41 0.47405203139022234 1102 42-43 0.5199269750815461 1102 44-45 0.5187476521677681 1102 46-47 0.474036982621854 1102 48-49 0.5013731803498018 1102 50-51 0.506045664578366 1102 52-53 0.43497505347585275 1102 54-55 0.582683645997875 1102 56-57 0.5516011218691901 1102 58-59 0.5898381368517391 1102 60-61 0.5897147622331786 1102 62-63 0.5867266151092707 1102 64-65 0.5770417735459432 1102 66-67 0.5704585069716472 1102 68-69 0.5701594796943397 1102 70-71 0.43612706219095543 1102 72-73 0.385927939894799 1102 74-75 0.4521831230973632 1102 76-77 0.5455672335822825 1102 78-79 0.5808153604323536 1102 80-81 0.4494483601982324 1102 82-83 0.47852892226281085 1102 84-85 0.5375303684495059 1102 86-87 0.5122999946722437 1102 88-89 0.45770518232788504 1102 90-91 0.4784736362279709 1102 92-93 0.5167547323861719 1102 94-95 0.43874028552882294 1102 96-97 0.4014140704293716 1102 98-99 0.36983926713209314 1102 100-101 0.6501011727393227 1103 1 0.08359251371230414 1103 2 0.1468782624863394 1103 3 0.1067181806085884 1103 4 0.1273499799533795 1103 5 0.11248324650943431 1103 6 0.1169763686355978 1103 7 0.08283023074619678 1103 8 0.15185253891564088 1103 9 0.14751940698067756 1103 10-11 0.13147165114047255 1103 12-13 0.14928608210972527 1103 14-15 0.061366311217291525 1103 16-17 0.08180888608096382 1103 18-19 0.08821618850814872 1103 20-21 0.04196329247039188 1103 22-23 0.02904845952641466 1103 24-25 0.08002822915363339 1103 26-27 0.12476136882096256 1103 28-29 0.1616092217946985 1103 30-31 0.16657999321537886 1103 32-33 0.11827740403243325 1103 34-35 0.1051616920387346 1103 36-37 0.1331244058333496 1103 38-39 0.10539121369528459 1103 40-41 0.19899628860704155 1103 42-43 0.15746036729586166 1103 44-45 0.09903735690248539 1103 46-47 0.12095755533389507 1103 48-49 0.16225895166482474 1103 50-51 0.0441710058323892 1103 52-53 0.13919910797507384 1103 54-55 0.2442552846025947 1103 56-57 0.16402683467166668 1103 58-59 0.23973332300979422 1103 60-61 0.24541601363134902 1103 62-63 0.24883833038858683 1103 64-65 0.32647613750685167 1103 66-67 0.3131677493663858 1103 68-69 0.27634316336720843 1103 70-71 0.23251482585113337 1103 72-73 0.2736822818612197 1103 74-75 0.3413839498303446 1103 76-77 0.2301078900308866 1103 78-79 0.29329272359861847 1103 80-81 0.27446100551124886 1103 82-83 0.22799492141430022 1103 84-85 0.1975428858196544 1103 86-87 0.15186840888280884 1103 88-89 0.0916239838197832 1103 90-91 0.02992127773834241 1103 92-93 0.10919421978741894 1103 94-95 0.1339293529678045 1103 96-97 0.0739203849068808 1103 98-99 -0.07230075799144942 1103 100-101 0.03491145565432063 1104 1 0.13837924153193626 1104 2 0.16406429199176387 1104 3 0.13219666312954104 1104 4 0.14697956878301 1104 5 0.14871744295415823 1104 6 0.2024461745789381 1104 7 0.1517397294934355 1104 8 0.14165758836408315 1104 9 0.186392883914543 1104 10-11 0.264911943435429 1104 12-13 0.23262673786512522 1104 14-15 0.19449821743167206 1104 16-17 0.1874041978695118 1104 18-19 0.34734949105821755 1104 20-21 0.3253218034899632 1104 22-23 0.31406252279855806 1104 24-25 0.29596749738839634 1104 26-27 0.4183041915367127 1104 28-29 0.3980027590221624 1104 30-31 0.35328526297818996 1104 32-33 0.3013501238738243 1104 34-35 0.33132338046223 1104 36-37 0.2837363173322558 1104 38-39 0.18356946338292346 1104 40-41 0.2086352886019256 1104 42-43 0.2239741815681242 1104 44-45 0.28230160428592654 1104 46-47 0.24008771463696377 1104 48-49 0.25364708531068914 1104 50-51 0.10882527202184633 1104 52-53 0.12663318436396764 1104 54-55 0.3341080738036979 1104 56-57 0.1900746648264473 1104 58-59 0.172433162227982 1104 60-61 0.2562339282704684 1104 62-63 0.23648141243909748 1104 64-65 0.30025774792446214 1104 66-67 0.19495416404340915 1104 68-69 0.1721479587215029 1104 70-71 0.17009305873050096 1104 72-73 0.10416357636111684 1104 74-75 0.07484901545998213 1104 76-77 0.13759969318684995 1104 78-79 0.09906310929202533 1104 80-81 0.17266401840132062 1104 82-83 0.16909894977467843 1104 84-85 0.12826412309714286 1104 86-87 0.10178422401417819 1104 88-89 0.11446214448214675 1104 90-91 0.1213372750530688 1104 92-93 0.17686372897987113 1104 94-95 0.11794472605754791 1104 96-97 0.14367142274410938 1104 98-99 0.21236866828547818 1104 100-101 0.47597968342197206 1105 1 -0.16218596370534755 1105 2 -0.009090693498663427 1105 3 0.009882473688129778 1105 4 0.05743141082351855 1105 5 0.10205621982702695 1105 6 0.17217611664073473 1105 7 0.17412484100987768 1105 8 0.13662090135424165 1105 9 0.17634729458994514 1105 10-11 0.11647702303994123 1105 12-13 0.11853791205454911 1105 14-15 0.09066068613902445 1105 16-17 0.16398919302933734 1105 18-19 0.20991993524196317 1105 20-21 0.04145686817442851 1105 22-23 0.2337278900567057 1105 24-25 0.17686957783308088 1105 26-27 0.2849271264788982 1105 28-29 0.3679434108040667 1105 30-31 0.3680943413757163 1105 32-33 0.3605566290804205 1105 34-35 0.30804081782511616 1105 36-37 0.31286870686471957 1105 38-39 0.335907148940791 1105 40-41 0.2388988379206083 1105 42-43 0.22039969148428185 1105 44-45 0.17851048109127277 1105 46-47 0.2159851479819963 1105 48-49 0.16813241184676286 1105 50-51 0.11492994692258662 1105 52-53 0.22989199546233863 1105 54-55 0.5229244386828142 1105 56-57 0.39532108252875986 1105 58-59 0.35320093539461084 1105 60-61 0.3942170175642943 1105 62-63 0.44275039443138553 1105 64-65 0.45749267742107236 1105 66-67 0.5195821458457459 1105 68-69 0.4481162703861443 1105 70-71 0.4064971678681246 1105 72-73 0.3800725994244374 1105 74-75 0.2704934418091298 1105 76-77 0.31954202605219706 1105 78-79 0.3951800559487424 1105 80-81 0.4808070616688198 1105 82-83 0.5103274391290071 1105 84-85 0.5446106413439473 1105 86-87 0.5204056141710787 1105 88-89 0.484187995411677 1105 90-91 0.374465582860676 1105 92-93 0.45805952865593724 1105 94-95 0.4453739028424266 1105 96-97 0.2850091037623592 1105 98-99 0.24889911574431522 1105 100-101 0.39560052953485325 1106 1 -0.18856630481050018 1106 2 -0.07458653766257584 1106 3 -0.08314622895570523 1106 4 0.03673784855632789 1106 5 -0.011864813967903842 1106 6 0.009947721783944985 1106 7 0.03476534620574512 1106 8 0.015670570105740467 1106 9 -0.08039035844351616 1106 10-11 -0.10916091788762827 1106 12-13 0.0701239760141803 1106 14-15 0.03348183354443535 1106 16-17 -0.06673857854961085 1106 18-19 0.12663865978940692 1106 20-21 0.1081258657510844 1106 22-23 0.2053130547983173 1106 24-25 0.14327437531628817 1106 26-27 0.16929760516173786 1106 28-29 0.1797941741851119 1106 30-31 0.2119783570054281 1106 32-33 0.23524249752696136 1106 34-35 -0.04701381662003712 1106 36-37 0.1333940373072906 1106 38-39 0.10776532418790907 1106 40-41 0.16746580247863818 1106 42-43 0.20564591336520266 1106 44-45 0.1848521333675066 1106 46-47 0.1664911612753528 1106 48-49 0.15287505559373926 1106 50-51 0.022181033669269823 1106 52-53 0.21197352588223595 1106 54-55 0.3793999659188856 1106 56-57 0.3365238245984372 1106 58-59 0.2965233621007144 1106 60-61 0.4219618498539788 1106 62-63 0.3940204222809456 1106 64-65 0.23115102621406436 1106 66-67 0.3720012214357098 1106 68-69 0.3497595138131828 1106 70-71 0.27680434705484913 1106 72-73 0.2423141083229794 1106 74-75 0.42849896018505973 1106 76-77 0.2686734439869767 1106 78-79 0.15499670207827876 1106 80-81 0.29645766275223195 1106 82-83 0.45082157921595467 1106 84-85 0.3233745383923612 1106 86-87 0.43854319998237656 1106 88-89 0.4792866423558557 1106 90-91 0.24268400473730978 1106 92-93 0.4320017192013097 1106 94-95 0.5418425154019353 1106 96-97 0.45461490892575895 1106 98-99 0.4938470420924794 1106 100-101 0.8153733609719431 1107 1 0.08664275373141095 1107 2 0.09917002705074651 1107 3 0.11484707264968108 1107 4 0.10057949878966355 1107 5 0.06208818447021969 1107 6 0.06775398145562406 1107 7 0.09733187075413952 1107 8 0.11183834231556489 1107 9 0.0966442907980678 1107 10-11 0.09481658533965032 1107 12-13 0.14837117476076145 1107 14-15 0.18071532423011405 1107 16-17 0.1545877605967334 1107 18-19 0.17932043148459087 1107 20-21 0.22843336108392265 1107 22-23 0.23507114721029154 1107 24-25 0.17261146168781494 1107 26-27 0.27680978130931777 1107 28-29 0.17612711501882217 1107 30-31 0.2495509621561709 1107 32-33 0.24978145913019745 1107 34-35 0.2908073614091151 1107 36-37 0.2541741107710891 1107 38-39 0.2706854662234264 1107 40-41 0.30811592040477365 1107 42-43 0.2743489036422204 1107 44-45 0.2998711522042683 1107 46-47 0.30076664696873223 1107 48-49 0.34877589434137946 1107 50-51 0.2956685192762194 1107 52-53 0.380739322229644 1107 54-55 0.5311705744864099 1107 56-57 0.48951921284278654 1107 58-59 0.5094731063629183 1107 60-61 0.5147293890775089 1107 62-63 0.5015604918028842 1107 64-65 0.4962373952890715 1107 66-67 0.44853428037180976 1107 68-69 0.4474330684574497 1107 70-71 0.36732662290285134 1107 72-73 0.44424581645345285 1107 74-75 0.5084156233650603 1107 76-77 0.5523810159806217 1107 78-79 0.5336563855926926 1107 80-81 0.5764838457416559 1107 82-83 0.5608574160715847 1107 84-85 0.6147625203634313 1107 86-87 0.5150900523453537 1107 88-89 0.5919886719973135 1107 90-91 0.5834227224421085 1107 92-93 0.6166171592261875 1107 94-95 0.663217621975317 1107 96-97 0.7541069123397897 1107 98-99 0.6534260329868289 1107 100-101 0.9243212795974678 1108 1 0.2651520825341578 1108 2 0.28774086837436386 1108 3 0.27198007482924424 1108 4 0.20294809871662522 1108 5 0.2739742826945033 1108 6 0.28187162136741506 1108 7 0.231037903949165 1108 8 0.25499923404125013 1108 9 0.31411379947414986 1108 10-11 0.3321534637597736 1108 12-13 0.37086452097304345 1108 14-15 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-1.8638328672484334 2316 56-57 -1.882225110052822 2316 58-59 -1.9270364368015436 2316 60-61 -1.8965200564778328 2316 62-63 -2.1613999145569025 2316 64-65 -1.7639117717075834 2316 66-67 -1.7663107676803236 2316 68-69 -1.9545938986402902 2316 70-71 -1.7831651542487776 2316 72-73 -1.7410481165520508 2316 74-75 -1.7964499818573856 2316 76-77 -1.929935206974264 2316 78-79 -1.7511403082874395 2316 80-81 -1.8830496254507096 2316 82-83 -1.8758084007791282 2316 84-85 -1.5000885447491008 2316 86-87 -1.5210626431851075 2316 88-89 -2.025105674126994 2316 90-91 -1.8016445297639443 2316 92-93 -1.7483830582650626 2316 94-95 -1.587966737397192 2316 96-97 -1.577926392227809 2316 98-99 -1.4981435407259767 2316 100-101 -1.6898590590907325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25160.0 3 7397.0 4 5938.0 5 4882.0 6 4370.0 7 4233.0 8 4773.0 9 5395.0 10 6069.0 11 7166.0 12 7914.0 13 8359.0 14 9367.0 15 10388.0 16 11985.0 17 14582.0 18 14091.0 19 12311.0 20 12428.0 21 13732.0 22 14741.0 23 16156.0 24 18624.0 25 21696.0 26 25556.0 27 30586.0 28 37406.0 29 45583.0 30 57093.0 31 71753.0 32 93405.0 33 125580.0 34 183120.0 35 293511.0 36 561023.0 37 1154365.0 38 1221433.0 39 164920.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.470896065947635 17.844043790953467 13.549944187592564 25.135115955506336 2 16.079320105455597 19.636204079330277 38.06869409461597 26.215781720598148 3 18.91218412676838 23.049456283923746 28.75642356572246 29.28193602358542 4 13.867095625585252 16.153433100646698 35.13468032536217 34.84479094840589 5 15.662917456887632 35.63310658047237 33.491735885900226 15.212240076739771 6 34.40359730373274 33.85446897214577 17.27384920791775 14.468084516203747 7 30.400459178767193 30.460006324572632 20.572013211329494 18.56752128533069 8 27.417760359253972 33.461444561992764 19.86342097670324 19.257374102050026 9 26.94262693929822 15.874719861488193 19.25203161823261 37.930621580980976 10-11 25.995707731861767 25.039448274331598 27.121029571064454 21.843814422742177 12-13 26.507024776385617 22.1695969508832 27.233080920042514 24.090297352688665 14-15 23.850472008466888 23.727632365581506 24.37037025049716 28.05152537545445 16-17 24.415579551361986 26.7799303461664 25.00444772338409 23.80004237908753 18-19 24.42076143607813 26.02462439446197 26.14213388683599 23.412480282623914 20-21 24.788751084854212 25.442680626781023 25.934916607829077 23.83365168053569 22-23 24.80202480230225 25.053240768035796 25.810460784946137 24.334273644715818 24-25 24.750955852898628 25.339469067950677 25.625155880127803 24.284419199022896 26-27 24.498703083127737 25.61557030708763 25.628052886376864 24.257673723407773 28-29 24.47186250608388 25.565013526991166 25.762136468252688 24.200987498672266 30-31 24.609051281440774 25.349408719608707 25.5966722692883 24.444867729662217 32-33 24.484333110355365 25.36544863363287 25.595015373226854 24.55520288278491 34-35 24.83004053179734 25.387368639835277 25.370641745173444 24.41194908319393 36-37 24.771616610877885 25.23835833040519 25.428171176002813 24.561853882714107 38-39 24.53945338367501 25.36499450471347 25.73083843857587 24.364713673035652 40-41 24.72761860074324 25.14006956238338 25.69165684791911 24.440654988954268 42-43 24.736605481472235 25.158741716190384 25.472838812725225 24.631813989612155 44-45 24.678571015799267 25.343446251285783 25.34730649653849 24.630676236376456 46-47 24.70865803217191 25.331351721794682 25.28637252987152 24.673617716161882 48-49 24.79455626696428 25.343585139350377 25.27742705620571 24.584431537479638 50-51 24.55952008920316 25.374881191890353 25.414892249828032 24.650706469078457 52-53 24.852849577680455 25.30729515881034 25.18797978839466 24.651875475114547 54-55 24.741407168478997 25.10441260570129 25.32719956468935 24.826980661130357 56-57 24.5826095830794 25.496277220165663 25.43362314934426 24.487490047410674 58-59 24.734812275210647 25.128119601487104 25.45748992720636 24.679578196095893 60-61 24.81111044464038 25.082730013592535 25.555631448465498 24.550528093301587 62-63 24.688532415200733 25.257187502347627 25.46587081305704 24.5884092693946 64-65 24.635147766945767 25.150788022574634 25.376132152578478 24.83793205790112 66-67 24.550504083321652 25.336553469190953 25.33302853222665 24.779913915260746 68-69 24.379832646438413 25.19970305890245 25.561078479155814 24.859385815503327 70-71 24.356209495968454 25.187798840500303 25.551357652657536 24.904634010873707 72-73 24.594064862243382 25.14697761141415 25.406374342666282 24.852583183676185 74-75 24.474571772902838 25.242713845375647 25.32684154470385 24.95587283701767 76-77 24.63486735554282 25.11936417407806 25.395258356301394 24.850510114077725 78-79 24.43541949892173 25.18565453611967 25.499923599907625 24.87900236505097 80-81 24.342218710747286 25.29040940479421 25.556871331308738 24.810500553149765 82-83 24.471297801796613 25.19716361300609 25.44235481556978 24.889183769627518 84-85 24.423495616689543 25.098792665092517 25.411699677695722 25.066012040522217 86-87 24.493871888862678 24.979095644347073 25.651571574144498 24.875460892645744 88-89 24.369678936213177 24.94215220497798 25.61783201987428 25.070336838934566 90-91 24.277389638404717 25.105835528560448 25.733497886756794 24.883276946278038 92-93 24.386421757112835 24.902462127561385 25.753061735490927 24.95805437983484 94-95 24.361325844055717 24.83995780082782 25.681651582070003 25.117064773046465 96-97 24.470972861692868 24.830325580340933 25.606607953921795 25.0920936040444 98-99 24.145547491965864 24.81191435174933 25.678401727886747 25.364136428398055 100-101 24.05583539968781 24.665518867564508 25.985911860778323 25.292733871969364 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 229.0 1 171.5 2 129.5 3 137.0 4 129.0 5 146.5 6 223.5 7 453.5 8 751.0 9 892.0 10 866.5 11 816.5 12 863.0 13 941.0 14 971.0 15 1021.5 16 1080.5 17 1107.5 18 1143.5 19 1211.0 20 1358.5 21 1526.0 22 1785.5 23 2228.5 24 2731.0 25 3413.0 26 4669.5 27 6518.0 28 8702.0 29 11673.5 30 16116.0 31 21500.0 32 27210.5 33 33397.5 34 40198.0 35 47210.0 36 55622.5 37 65183.0 38 75342.0 39 83462.5 40 90872.0 41 103595.5 42 116227.0 43 128952.5 44 142452.5 45 153314.0 46 162286.0 47 171918.5 48 186265.5 49 197369.0 50 202738.0 51 205384.0 52 205025.5 53 205445.0 54 203660.0 55 194188.0 56 180937.0 57 166684.0 58 150402.0 59 131933.5 60 111737.5 61 90980.0 62 72259.0 63 56891.0 64 43898.0 65 32915.0 66 24282.5 67 18765.5 68 14125.5 69 10357.0 70 7727.0 71 5514.5 72 4132.5 73 3070.0 74 2274.5 75 1778.0 76 1224.0 77 755.5 78 468.5 79 292.5 80 194.0 81 144.5 82 109.0 83 81.5 84 69.5 85 61.5 86 49.0 87 44.5 88 39.0 89 32.0 90 29.0 91 25.5 92 18.0 93 20.0 94 21.0 95 15.5 96 12.5 97 8.5 98 9.0 99 6.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023271905056173804 2 0.03452654037132439 3 0.02410386988439849 4 0.022486160496183825 5 0.04249953664181094 6 0.022578601032653235 7 0.02592957047966932 8 0.039241007731264266 9 0.025906460345551968 10-11 0.027454839331414575 12-13 0.026160671820842845 14-15 0.035774487613661414 16-17 0.042973294391216665 18-19 0.04445234297472722 20-21 0.03790061995245783 22-23 0.04490299059001559 24-25 0.019990266011509772 26-27 0.024889614444388468 28-29 0.026010455949080056 30-31 0.02669220490554195 32-33 0.03620202509483243 34-35 0.04013074789478233 36-37 0.029257429792568058 38-39 0.015506899992743418 40-41 0.017875688739772033 42-43 0.020706680169147694 44-45 0.022093288216188833 46-47 0.015275798651569894 48-49 0.02551358806555698 50-51 0.018534327562116572 52-53 0.027165962654947674 54-55 0.023110134117352337 56-57 0.022428385160890442 58-59 0.02247460542912515 60-61 0.019285406920930524 62-63 0.021573310198548405 64-65 0.01927385185387185 66-67 0.018199230617414967 68-69 0.023006138513824253 70-71 0.035439390668959805 72-73 0.025582918467909038 74-75 0.035566496406605246 76-77 0.043839924420617384 78-79 0.027616610270236045 80-81 0.022463050362066472 82-83 0.015865107071562377 84-85 0.031891985081946225 86-87 0.01974760960327757 88-89 0.03037827129725965 90-91 0.024358081359689362 92-93 0.04305417986062741 94-95 0.07682964087313784 96-97 0.09693545755523438 98-99 0.07421819571787704 100-101 0.08016905525309526 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4327106.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.92087931067449 #Duplication Level Percentage of deduplicated Percentage of total 1 76.24255786120062 38.823380871873134 2 11.946770782904323 12.166801463771268 3 4.278420831351517 6.5358285238057885 4 2.1151656700986026 4.308243832366916 5 1.2741987088012519 3.2441659334342914 6 0.8335546554487865 2.5467201605375114 7 0.5730034890294283 2.0424489056624044 8 0.43074810503148114 1.7547257815687833 9 0.33928205240108644 1.5548886398334298 >10 1.798082297436126 16.3467936756645 >50 0.10843534108979974 3.807000483382316 >100 0.056056320748303676 5.189608500824983 >500 0.002724793665227564 0.9516785855599819 >1k 9.990907933406451E-4 0.7277146417146609 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.3110134117352336E-5 2.3110134117352336E-5 6 2.3110134117352336E-5 0.0 0.0 2.3110134117352336E-5 2.3110134117352336E-5 7 2.3110134117352336E-5 0.0 0.0 2.3110134117352336E-5 2.3110134117352336E-5 8 2.3110134117352336E-5 0.0 0.0 2.3110134117352336E-5 2.3110134117352336E-5 9 2.3110134117352336E-5 0.0 0.0 2.3110134117352336E-5 2.3110134117352336E-5 10-11 3.466520117602851E-5 0.0 0.0 2.3110134117352336E-5 2.3110134117352336E-5 12-13 4.622026823470467E-5 1.1555067058676168E-5 0.0 3.466520117602851E-5 2.3110134117352336E-5 14-15 4.622026823470467E-5 2.3110134117352336E-5 0.0 4.622026823470467E-5 2.3110134117352336E-5 16-17 5.7775335293380844E-5 2.3110134117352336E-5 0.0 4.622026823470467E-5 3.466520117602851E-5 18-19 6.933040235205702E-5 2.3110134117352336E-5 0.0 4.622026823470467E-5 4.622026823470467E-5 20-21 6.933040235205702E-5 2.3110134117352336E-5 0.0 5.7775335293380844E-5 4.622026823470467E-5 22-23 6.933040235205702E-5 2.3110134117352336E-5 0.0 9.244053646940934E-5 4.622026823470467E-5 24-25 9.244053646940934E-5 2.3110134117352336E-5 0.0 1.1555067058676169E-4 4.622026823470467E-5 26-27 9.244053646940934E-5 2.3110134117352336E-5 0.0 1.1555067058676169E-4 5.7775335293380844E-5 28-29 9.244053646940934E-5 2.3110134117352336E-5 0.0 6.124185541098369E-4 6.933040235205702E-5 30-31 9.244053646940934E-5 2.3110134117352336E-5 0.0 0.0023803438140872907 6.933040235205702E-5 32-33 9.244053646940934E-5 2.3110134117352336E-5 0.0 0.0074183530516700995 6.933040235205702E-5 34-35 1.0399560352808551E-4 2.3110134117352336E-5 0.0 0.014455388890403886 6.933040235205702E-5 36-37 1.3866080470411403E-4 2.3110134117352336E-5 0.0 0.02702730185024356 8.088546941073318E-5 38-39 1.6177093882146636E-4 2.3110134117352336E-5 0.0 0.045700290217064246 1.2710573764543787E-4 40-41 1.6177093882146636E-4 2.3110134117352336E-5 0.0 0.06600254303915828 1.3866080470411403E-4 42-43 1.6177093882146636E-4 2.3110134117352336E-5 0.0 0.08815360659064049 1.6177093882146636E-4 44-45 1.6177093882146636E-4 2.3110134117352336E-5 0.0 0.11559689085499639 1.7332600588014252E-4 46-47 1.9643613999749485E-4 2.3110134117352336E-5 0.0 0.14825151036281525 2.0799120705617102E-4 48-49 2.0799120705617102E-4 2.3110134117352336E-5 0.0 0.18254694939296612 2.426564082321995E-4 50-51 2.0799120705617102E-4 2.3110134117352336E-5 0.0 0.21907251636544148 2.7732160940822806E-4 52-53 2.0799120705617102E-4 2.3110134117352336E-5 0.0 0.2576317751402438 3.004317435255804E-4 54-55 2.0799120705617102E-4 4.622026823470467E-5 0.0 0.3016334704996827 3.004317435255804E-4 56-57 2.542114752908757E-4 4.622026823470467E-5 0.0 0.35517965124958806 3.004317435255804E-4 58-59 2.542114752908757E-4 6.933040235205702E-5 0.0 0.41423759898648194 3.004317435255804E-4 60-61 2.542114752908757E-4 9.244053646940934E-5 0.0 0.47809089955272643 3.004317435255804E-4 62-63 2.542114752908757E-4 9.244053646940934E-5 0.0 0.5467511080153802 3.004317435255804E-4 64-65 2.542114752908757E-4 9.244053646940934E-5 0.0 0.6262499693790723 3.235418776429327E-4 66-67 2.542114752908757E-4 9.244053646940934E-5 0.0 0.7229312154590157 3.235418776429327E-4 68-69 2.542114752908757E-4 9.244053646940934E-5 0.0 0.8320110484929188 3.235418776429327E-4 70-71 2.7732160940822806E-4 1.0399560352808551E-4 0.0 0.951363336141985 3.350969447016089E-4 72-73 2.7732160940822806E-4 1.1555067058676169E-4 0.0 1.0793703690179997 3.8131721293631354E-4 74-75 2.7732160940822806E-4 1.1555067058676169E-4 0.0 1.225796178785544 3.928722799949897E-4 76-77 2.7732160940822806E-4 1.1555067058676169E-4 0.0 1.3996537177503856 3.928722799949897E-4 78-79 2.7732160940822806E-4 1.1555067058676169E-4 0.0 1.592461566691456 3.928722799949897E-4 80-81 3.004317435255804E-4 1.3866080470411403E-4 0.0 1.8018971571299618 4.1598241411234204E-4 82-83 3.004317435255804E-4 1.3866080470411403E-4 0.0 2.028434246815308 4.6220268234704675E-4 84-85 3.1198681058425655E-4 1.3866080470411403E-4 0.0 2.2799649465485707 4.6220268234704675E-4 86-87 3.235418776429327E-4 1.3866080470411403E-4 0.0 2.559562904167358 4.737577494057229E-4 88-89 3.235418776429327E-4 1.3866080470411403E-4 0.0 2.8773272482809524 4.853128164643991E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3390 0.0 40.490467 1 GTATCAA 5565 0.0 39.174385 1 TCAACGC 6775 0.0 31.338486 4 CAACGCA 6730 0.0 31.33702 5 ATCAACG 6765 0.0 31.104681 3 TATCAAC 7175 0.0 29.657932 2 AACGCAG 7160 0.0 29.38769 6 GTACATG 11195 0.0 26.388878 1 ACGCAGA 8435 0.0 25.001877 7 TACATGG 11740 0.0 24.15411 2 CGCAGAG 8990 0.0 23.511215 8 ACATGGG 12045 0.0 22.990667 3 CATGGGG 10390 0.0 20.023422 4 GTCCTAA 1130 0.0 18.914234 1 GCAGAGT 11240 0.0 18.428728 9 AGAGTAC 9625 0.0 17.52257 10-11 CAGAGTA 10050 0.0 16.781565 10-11 ATGGGGA 6455 0.0 16.188858 5 GAGTACA 8640 0.0 16.106773 1 CCTATAA 1930 0.0 15.749883 1 >>END_MODULE