FastQCFastQC Report
Fri 27 May 2016
SRR940212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940212_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4327106
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34650.042.7935521
GTATCAA56400.040.2787251
ATCAACG63050.034.276563
TCAACGC63750.033.975094
CAACGCA65500.033.140265
TATCAAC69800.031.3715882
AACGCAG69700.031.211076
ACGCAGA82550.026.2375777
GTACATG109300.024.7845861
CGCAGAG88050.024.5447188
TACATGG115000.022.9237772
ACATGGG116100.022.132783
CATGGGG97850.019.8535634
GCAGAGT110450.019.3088579
CAGAGTA99900.017.63932210-11
TATAAGC20250.017.3571193
AGAGTAC92500.017.1248810-11
CCTATAA21000.016.9734041
AATTCGC6250.015.95990578-79
GAGTACA85000.015.8791181
Produced by FastQC (version 0.11.5)