FastQCFastQC Report
Fri 27 May 2016
SRR940211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4450676
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35100.041.1542551
GTATCAA57700.038.1288261
ATCAACG67000.033.8280643
TCAACGC67600.033.3853654
CAACGCA69800.032.331655
AACGCAG74350.030.4685176
TATCAAC71600.029.930352
ACGCAGA88600.025.6219967
GTACATG119400.025.4299071
CGCAGAG93800.024.0997838
ACATGGG128600.023.3512273
TACATGG126200.023.3442862
CATGGGG109900.021.5298424
GCAGAGT115800.019.5629129
AGAGTAC101100.017.52328510-11
ATGGGGA66600.017.4775875
CAGAGTA104550.017.26304610-11
GTATAGA9250.015.9245881
GTGGTAT19700.015.9193741
AGTACAT86700.015.5101352
Produced by FastQC (version 0.11.5)