##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940211_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4450676 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96789251790065 33.0 31.0 34.0 30.0 34.0 2 30.936132174078725 34.0 31.0 34.0 26.0 34.0 3 31.785845341247036 34.0 31.0 34.0 28.0 34.0 4 35.527855993112055 37.0 35.0 37.0 33.0 37.0 5 35.692265174998134 37.0 35.0 37.0 35.0 37.0 6 35.847635505258076 37.0 37.0 37.0 35.0 37.0 7 35.82334189233276 37.0 37.0 37.0 35.0 37.0 8 35.81875831896099 37.0 37.0 37.0 35.0 37.0 9 37.58857575792981 39.0 39.0 39.0 35.0 39.0 10-11 37.53139343326721 39.0 38.0 39.0 35.0 39.0 12-13 37.457292667450965 39.0 38.0 39.0 35.0 39.0 14-15 38.90845356974985 41.0 39.0 41.0 36.0 41.0 16-17 38.826721378954566 41.0 39.0 41.0 36.0 41.0 18-19 38.75508181229099 41.0 39.0 41.0 35.5 41.0 20-21 38.68190247953345 41.0 39.0 41.0 35.0 41.0 22-23 38.58306187644304 40.5 39.0 41.0 35.0 41.0 24-25 38.478532362274855 40.0 38.5 41.0 34.5 41.0 26-27 38.29301941997126 40.0 38.0 41.0 34.0 41.0 28-29 38.13054668549227 40.0 38.0 41.0 34.0 41.0 30-31 38.11583094343421 40.0 38.0 41.0 34.0 41.0 32-33 38.15199207940546 40.0 38.0 41.0 34.0 41.0 34-35 38.16085578909811 40.0 38.0 41.0 34.0 41.0 36-37 38.26797344942656 40.5 38.0 41.0 34.0 41.0 38-39 38.19465672181035 40.5 38.0 41.0 34.0 41.0 40-41 38.07716490708378 40.0 38.0 41.0 33.5 41.0 42-43 37.808272383790694 40.0 38.0 41.0 33.0 41.0 44-45 37.76047829588134 40.0 38.0 41.0 33.0 41.0 46-47 37.60719888394482 40.0 38.0 41.0 33.0 41.0 48-49 37.4294929579237 40.0 37.5 41.0 32.5 41.0 50-51 36.490727925375836 39.0 36.0 40.0 31.0 40.5 52-53 36.55718940223912 39.0 36.5 40.5 31.0 41.0 54-55 36.904056934272454 40.0 36.5 41.0 32.0 41.0 56-57 36.71621827785262 39.5 36.0 41.0 31.0 41.0 58-59 36.441582694404175 39.0 35.0 41.0 31.0 41.0 60-61 36.161317629052306 39.0 35.0 41.0 31.0 41.0 62-63 35.80598486611922 38.5 35.0 40.5 30.0 41.0 64-65 35.420554203451346 37.5 35.0 40.0 30.0 41.0 66-67 35.03821453190481 37.0 35.0 39.5 29.0 41.0 68-69 34.647128548562065 36.0 35.0 39.0 29.0 41.0 70-71 34.23107939108576 36.0 34.0 39.0 29.0 40.5 72-73 33.79733494417478 35.0 34.0 37.5 28.5 40.0 74-75 33.37283134067724 35.0 34.0 37.0 27.5 39.0 76-77 32.949254225650215 35.0 34.0 36.5 26.5 39.0 78-79 32.54502360989656 35.0 33.0 36.0 26.0 38.0 80-81 32.18269449404989 35.0 33.0 36.0 26.0 37.0 82-83 31.861166820500976 35.0 33.0 35.0 25.0 37.0 84-85 31.58685231187352 35.0 33.0 35.0 24.5 36.0 86-87 31.305958690320303 35.0 32.5 35.0 24.0 36.0 88-89 31.02003763023864 35.0 32.0 35.0 22.5 36.0 90-91 30.761256267587214 34.5 32.0 35.0 20.0 35.0 92-93 30.485476925303033 34.0 31.5 35.0 18.5 35.0 94-95 30.17995816815243 34.0 31.0 35.0 15.0 35.0 96-97 29.82267154472714 34.0 31.0 35.0 4.5 35.0 98-99 29.357257077351846 34.0 31.0 35.0 2.0 35.0 100-101 27.334441329811472 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.511837866329607 1101 2 1.573883949134693 1101 3 0.8552867426437736 1101 4 0.4404797870184325 1101 5 0.3199503148095033 1101 6 0.28934137509963875 1101 7 0.2503530797291802 1101 8 0.2394751678819702 1101 9 0.27256285488805787 1101 10-11 0.22857934984703832 1101 12-13 0.3306700993439904 1101 14-15 0.3788799075407496 1101 16-17 0.36627513696934244 1101 18-19 0.3604719619367529 1101 20-21 0.4296905997326306 1101 22-23 0.4119078496744919 1101 24-25 0.37788273937059813 1101 26-27 0.4866536591948005 1101 28-29 0.45943799916284433 1101 30-31 0.4522188578814834 1101 32-33 0.36885857500438135 1101 34-35 0.4134777366767324 1101 36-37 0.45027448628484024 1101 38-39 0.4495278175617301 1101 40-41 0.47104581728007844 1101 42-43 0.636925307391671 1101 44-45 0.5504157858302037 1101 46-47 0.5893345343840082 1101 48-49 0.5360907550406111 1101 50-51 0.3768314623852973 1101 52-53 0.4892984224877637 1101 54-55 0.5293751062875458 1101 56-57 0.6025185627585259 1101 58-59 0.6072340193548413 1101 60-61 0.5622481545044309 1101 62-63 0.6342597144148314 1101 64-65 0.6299673854401178 1101 66-67 0.5951748310287641 1101 68-69 0.5338614336888909 1101 70-71 0.5011697110688402 1101 72-73 0.5547542901323439 1101 74-75 0.5110837150993532 1101 76-77 0.6053434877450812 1101 78-79 0.5910307074643839 1101 80-81 0.6191221957949224 1101 82-83 0.7083556434047615 1101 84-85 0.5859043965992967 1101 86-87 0.5092292235561118 1101 88-89 0.555627106862957 1101 90-91 0.43190429717220624 1101 92-93 0.3752664528703029 1101 94-95 0.40837202323460176 1101 96-97 0.33536675187272635 1101 98-99 0.3571429426624029 1101 100-101 0.5043191721136786 1102 1 0.3938745549541167 1102 2 0.9021554671545537 1102 3 0.593816061345958 1102 4 0.3982342951117417 1102 5 0.38266033713248504 1102 6 0.269842827973811 1102 7 0.31081835009387504 1102 8 0.3707321600618556 1102 9 0.35115111797647103 1102 10-11 0.4090691170982055 1102 12-13 0.4058264890100105 1102 14-15 0.4480523272457617 1102 16-17 0.41482871920825204 1102 18-19 0.42659490557240076 1102 20-21 0.39236129113823637 1102 22-23 0.42632862181548603 1102 24-25 0.44050731095316564 1102 26-27 0.4592848177690101 1102 28-29 0.45072135865432017 1102 30-31 0.47431157704313875 1102 32-33 0.3593097343596483 1102 34-35 0.44420602847079493 1102 36-37 0.5157595266924346 1102 38-39 0.4201597854847279 1102 40-41 0.441548989446126 1102 42-43 0.5858549937950173 1102 44-45 0.4032931573980747 1102 46-47 0.373266397710708 1102 48-49 0.4123847428268661 1102 50-51 0.21495898427029658 1102 52-53 0.3496423812141103 1102 54-55 0.42592923188072973 1102 56-57 0.4564201643719983 1102 58-59 0.48214734443509144 1102 60-61 0.5362086811590814 1102 62-63 0.5348210474923221 1102 64-65 0.517533012031933 1102 66-67 0.48722724113863336 1102 68-69 0.4777106549355139 1102 70-71 0.5161882152196284 1102 72-73 0.5520387580955415 1102 74-75 0.3969441052141818 1102 76-77 0.4299630823283209 1102 78-79 0.4295604251583498 1102 80-81 0.38271859650213 1102 82-83 0.4082839881075522 1102 84-85 0.3876810767869472 1102 86-87 0.40927153482120815 1102 88-89 0.3392278070089034 1102 90-91 0.26104679612062043 1102 92-93 0.2786579374425635 1102 94-95 0.2622518616598555 1102 96-97 0.21146036719410688 1102 98-99 0.1931999390014738 1102 100-101 0.37532521901552585 1103 1 0.3348237285531326 1103 2 1.404761832142622 1103 3 0.6445086599486523 1103 4 0.29257948440961457 1103 5 0.19631619061483718 1103 6 0.1638385500452415 1103 7 0.11228624126996323 1103 8 0.16369238638170458 1103 9 0.18820283022534312 1103 10-11 0.18214871036707336 1103 12-13 0.23326439767819807 1103 14-15 0.21687615571076435 1103 16-17 0.22516381424366472 1103 18-19 0.13401245871155254 1103 20-21 0.12509656784038725 1103 22-23 0.2587129146690188 1103 24-25 0.21667536768116236 1103 26-27 0.21378576006569006 1103 28-29 0.2430960165652465 1103 30-31 0.24582143707823434 1103 32-33 0.11129984715417862 1103 34-35 0.28943637468693595 1103 36-37 0.23509975037019615 1103 38-39 0.2025592562714209 1103 40-41 0.27845155742124206 1103 42-43 0.40670754157974187 1103 44-45 0.22403465213404417 1103 46-47 0.13829805588116528 1103 48-49 0.15547172714018842 1103 50-51 0.0814005213545812 1103 52-53 0.17139497692176064 1103 54-55 0.29420555973907625 1103 56-57 0.3578775211724192 1103 58-59 0.3060617708731712 1103 60-61 0.36417295727004273 1103 62-63 0.30203459211123374 1103 64-65 0.336290981617978 1103 66-67 0.302597199745982 1103 68-69 0.3103457174601658 1103 70-71 0.37373710549722006 1103 72-73 0.4056550587741512 1103 74-75 0.45043299791836944 1103 76-77 0.5108570450243306 1103 78-79 0.5107198552925425 1103 80-81 0.41320349281904356 1103 82-83 0.45019862347644235 1103 84-85 0.410424524510681 1103 86-87 0.3978186658170806 1103 88-89 0.37615222839704643 1103 90-91 0.3699816682652717 1103 92-93 0.33303654278725503 1103 94-95 0.24734097697373514 1103 96-97 0.13399607866909236 1103 98-99 0.18330939579074013 1103 100-101 0.4123216653179682 1104 1 0.43658921398198913 1104 2 1.4481232628802907 1104 3 0.7399785109878074 1104 4 0.3869510837317094 1104 5 0.2611074670397784 1104 6 0.185857740381266 1104 7 0.22487894359039018 1104 8 0.2169930349793816 1104 9 0.16819617222363803 1104 10-11 0.1446600194130383 1104 12-13 0.08124852041480324 1104 14-15 0.12419175454863307 1104 16-17 0.11307364769805162 1104 18-19 0.1201114909120733 1104 20-21 0.15382074817348013 1104 22-23 0.10294713297501801 1104 24-25 0.13108898568388838 1104 26-27 0.1262987415761998 1104 28-29 0.07359890276120495 1104 30-31 -0.007433497488584351 1104 32-33 0.08512498063277718 1104 34-35 0.0027385205385925815 1104 36-37 0.09231747021168246 1104 38-39 0.20218295174908008 1104 40-41 0.21471313724718044 1104 42-43 0.21480019930110927 1104 44-45 0.1125103360924058 1104 46-47 0.09079847001524399 1104 48-49 0.04118625177797952 1104 50-51 0.0469043473163282 1104 52-53 0.07463852280144323 1104 54-55 0.16470185164626372 1104 56-57 0.14390200923897822 1104 58-59 0.125260389576745 1104 60-61 0.145561931109512 1104 62-63 0.18917588828718124 1104 64-65 0.1937818706682677 1104 66-67 0.16460015921180826 1104 68-69 0.028427129848502375 1104 70-71 0.023999284224750284 1104 72-73 0.03554940679968155 1104 74-75 -0.03531083466952367 1104 76-77 0.04077703750620287 1104 78-79 0.033626248248445734 1104 80-81 -0.013096967825468653 1104 82-83 -0.008720868858890896 1104 84-85 -0.044737895688868434 1104 86-87 -0.05498080090565338 1104 88-89 -0.12468081822807875 1104 90-91 -0.16735490805644915 1104 92-93 -0.1292019952557375 1104 94-95 -0.09392421856126631 1104 96-97 -0.23021453399448788 1104 98-99 -0.17928489935306047 1104 100-101 -0.17678405992637636 1105 1 0.19703781207582693 1105 2 1.1579128012662956 1105 3 0.44465572168598655 1105 4 0.19352676779216438 1105 5 0.04863853445223043 1105 6 -0.012099209167537595 1105 7 -0.022708001448208393 1105 8 0.02459481385165674 1105 9 -0.12835496144616343 1105 10-11 -0.08926716216846842 1105 12-13 -0.16990451651240335 1105 14-15 -0.12563874184699841 1105 16-17 -0.2713635788897477 1105 18-19 -0.3605080196044099 1105 20-21 -0.23158747424428583 1105 22-23 -0.28790871258154027 1105 24-25 -0.2691293976459974 1105 26-27 -0.1958179072575419 1105 28-29 -0.23470580054727463 1105 30-31 -0.3178891231771175 1105 32-33 -0.22000988154884027 1105 34-35 -0.1629294358401765 1105 36-37 -0.20491250271064132 1105 38-39 -0.17500430461527117 1105 40-41 -0.2387471118068163 1105 42-43 -0.13093032246645464 1105 44-45 -0.1823108710108059 1105 46-47 -0.2671851415019688 1105 48-49 -0.375817112559254 1105 50-51 -0.41836523022625727 1105 52-53 -0.3044088191015959 1105 54-55 -0.1971094894398675 1105 56-57 -0.17611782639448137 1105 58-59 -0.14972221465188795 1105 60-61 -0.3388084343485076 1105 62-63 -0.3794925375877014 1105 64-65 -0.2607462923349786 1105 66-67 -0.31268091389200237 1105 68-69 -0.4235734086038221 1105 70-71 -0.46414857834717793 1105 72-73 -0.6306716839687923 1105 74-75 -0.6309286014846904 1105 76-77 -0.6372291316001579 1105 78-79 -0.45249557197116275 1105 80-81 -0.5536645830194651 1105 82-83 -0.5568964326913921 1105 84-85 -0.46356983686558806 1105 86-87 -0.4973113030751364 1105 88-89 -0.5673405120691015 1105 90-91 -0.6352348411062394 1105 92-93 -0.6721563531727845 1105 94-95 -0.6571531871644147 1105 96-97 -0.793793067695109 1105 98-99 -0.9081402526480638 1105 100-101 -0.8885310968713966 1106 1 0.30033475232604445 1106 2 1.3053905117073903 1106 3 0.5568208510507908 1106 4 0.34632608258333164 1106 5 0.1439327053591768 1106 6 0.14170824693286477 1106 7 0.1949332533304613 1106 8 0.1615373112531273 1106 9 0.040998233634546466 1106 10-11 0.1374166383551909 1106 12-13 0.05471854863351666 1106 14-15 0.09221041366875227 1106 16-17 0.02343639369316719 1106 18-19 -0.020877939285817604 1106 20-21 -0.06825249188630522 1106 22-23 -0.03573875431986551 1106 24-25 -0.00912157250588308 1106 26-27 0.10048673213785975 1106 28-29 -0.009712841173453057 1106 30-31 0.022209665580646742 1106 32-33 0.0903613744425158 1106 34-35 0.018588997691864506 1106 36-37 0.053259032578580445 1106 38-39 0.02841888510747026 1106 40-41 0.1019204722775342 1106 42-43 0.2335362144236086 1106 44-45 0.15681977483050957 1106 46-47 0.0930332024518492 1106 48-49 -0.059639271705215435 1106 50-51 -0.23254487864402051 1106 52-53 -0.013819950036150885 1106 54-55 0.1134619403263244 1106 56-57 0.1654085413641937 1106 58-59 0.04955077819694509 1106 60-61 0.1188180864767503 1106 62-63 0.13469389237575058 1106 64-65 -0.03331343100085604 1106 66-67 0.09801349275092974 1106 68-69 0.12047954854099174 1106 70-71 -0.09147009870062561 1106 72-73 0.0013200123648644535 1106 74-75 -0.024898589236094892 1106 76-77 -0.1010566759842888 1106 78-79 -0.0822643576254194 1106 80-81 -0.17350831293504143 1106 82-83 -0.0712175845141978 1106 84-85 -0.12090033322201066 1106 86-87 -0.13385872544234445 1106 88-89 -0.3411637820795228 1106 90-91 -0.5582275717619716 1106 92-93 -0.6983188473707465 1106 94-95 -0.6920155136054795 1106 96-97 -0.7998947316100633 1106 98-99 -0.9016546950677373 1106 100-101 -0.7154347794688789 1107 1 0.3537871975166027 1107 2 1.3764975288783212 1107 3 0.7146516925916835 1107 4 0.3994396512697804 1107 5 0.22950421880286598 1107 6 0.20687929308598285 1107 7 0.14333296481668611 1107 8 0.21745956514887865 1107 9 0.14790036242287385 1107 10-11 0.2446672728856356 1107 12-13 0.29029142470523084 1107 14-15 0.2681952757798811 1107 16-17 0.28297546205531177 1107 18-19 0.25212930807840905 1107 20-21 0.2568134320572497 1107 22-23 0.2508323830384924 1107 24-25 0.2132835567893494 1107 26-27 0.3492338592637907 1107 28-29 0.33556881403805505 1107 30-31 0.21793911669590926 1107 32-33 0.1694480503023854 1107 34-35 0.25984036259092846 1107 36-37 0.28186528213572615 1107 38-39 0.31476215472432045 1107 40-41 0.23026487923456784 1107 42-43 0.35374584111803387 1107 44-45 0.16569019253959283 1107 46-47 0.1590919916750977 1107 48-49 0.2076727355911956 1107 50-51 0.2012901924942554 1107 52-53 0.21187837865515746 1107 54-55 0.2661802742137951 1107 56-57 0.2598491643940619 1107 58-59 0.35944676425816624 1107 60-61 0.33521333581042256 1107 62-63 0.22534364792029038 1107 64-65 0.1897259925529866 1107 66-67 0.3931057790045571 1107 68-69 0.2869100990245457 1107 70-71 0.18369566045577557 1107 72-73 0.25157991094899756 1107 74-75 0.27884435007972286 1107 76-77 0.30872110663839436 1107 78-79 0.2750237945964855 1107 80-81 0.25136124722680364 1107 82-83 0.3166566249344491 1107 84-85 0.14319496353111916 1107 86-87 0.16787337462178797 1107 88-89 0.2669677642125805 1107 90-91 0.2257636565472616 1107 92-93 0.009666298167008591 1107 94-95 0.15666628004903416 1107 96-97 0.2297158656388767 1107 98-99 0.2655146904960368 1107 100-101 0.08144200443830485 1108 1 0.2624558301618478 1108 2 1.3875279106689327 1108 3 0.6112354640292601 1108 4 0.2882397227599114 1108 5 0.13387059966047588 1108 6 0.03424153448331424 1108 7 -0.030563322997728903 1108 8 0.029332985209435947 1108 9 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46-47 -1.962596827617574 2316 48-49 -2.014305553547011 2316 50-51 -2.164608095347347 2316 52-53 -2.354846353200614 2316 54-55 -2.4659215302039286 2316 56-57 -2.495409903512538 2316 58-59 -2.4356636414708817 2316 60-61 -2.415073553267689 2316 62-63 -2.1408019946346997 2316 64-65 -1.996967421004392 2316 66-67 -2.1058921601217477 2316 68-69 -2.073133312725055 2316 70-71 -1.9683109921891315 2316 72-73 -1.9929138908455677 2316 74-75 -1.9907359323584686 2316 76-77 -2.1046111877386515 2316 78-79 -2.3104449887150516 2316 80-81 -2.3039056751893483 2316 82-83 -2.3873391760198146 2316 84-85 -1.9526199543019835 2316 86-87 -1.9038200451693328 2316 88-89 -1.9734216947862535 2316 90-91 -1.693815715765215 2316 92-93 -2.0412567064447096 2316 94-95 -2.037170423450707 2316 96-97 -1.757191514732579 2316 98-99 -1.714780090494056 2316 100-101 -1.962837431582976 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30390.0 3 9116.0 4 6997.0 5 5855.0 6 5183.0 7 4914.0 8 5322.0 9 5995.0 10 6888.0 11 7944.0 12 8575.0 13 9351.0 14 10214.0 15 11662.0 16 13546.0 17 16257.0 18 15316.0 19 13184.0 20 14206.0 21 14936.0 22 15624.0 23 17392.0 24 19635.0 25 22893.0 26 26710.0 27 32441.0 28 39474.0 29 48418.0 30 59538.0 31 74648.0 32 96694.0 33 131436.0 34 189658.0 35 304952.0 36 585898.0 37 1196499.0 38 1214652.0 39 158257.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66382739838808 17.7843095861332 13.459242257772466 25.092620757706257 2 17.430678890502545 19.93693993444451 37.17234619413957 25.46003498091337 3 18.92820616241662 23.17656710582434 28.720480287816564 29.174746443942478 4 13.738965743697015 16.035736792560364 35.14389976467297 35.08139769906964 5 15.587655974695572 35.73580622120422 33.49210428162216 15.18443352247805 6 34.348536474708155 33.93636698050903 17.258153785377488 14.456942759405328 7 30.37119849349838 30.446074140823292 20.67277971346357 18.509947652214752 8 27.413140948429294 33.69583781276629 19.81050200810433 19.080519230700084 9 27.045562299034465 15.905976779563138 19.258918881266585 37.78954204013581 10-11 26.062752541751305 25.116061203148988 26.98409630908577 21.837089946013943 12-13 26.60115442489092 22.196825264134358 27.207726368132374 23.99429394284235 14-15 23.918669185239004 23.62805431298022 24.375625789979967 28.07765071180081 16-17 24.48852098998265 26.64770383276475 24.976819020012343 23.88695615724026 18-19 24.504888186855545 25.89778207708272 26.128605006902212 23.468724729159522 20-21 24.853188322491796 25.378369403431645 25.940646019703 23.82779625437356 22-23 24.865348395226142 24.987791632779505 25.82137647458311 24.32548349741124 24-25 24.73715083538403 25.234238852792224 25.708946449563104 24.319663862260647 26-27 24.58199896475132 25.541171359048715 25.63016858913492 24.246661087065043 28-29 24.50810293452223 25.53865718946879 25.753856010729375 24.199383865279607 30-31 24.584333115539522 25.310726972518093 25.64853022886328 24.456409683079105 32-33 24.49766877320187 25.355223590797166 25.620358923754605 24.526748712246363 34-35 24.8563936688371 25.347901008578905 25.408378660670593 24.387326661913406 36-37 24.770766013061905 25.203911807574357 25.455549209247952 24.569772970115782 38-39 24.56958608312044 25.359720353701515 25.758725520443722 24.31196804273433 40-41 24.719529715732662 25.12744767912251 25.77059316637977 24.38242943876506 42-43 24.819224179096093 25.16152917052892 25.48421673548496 24.535029914890032 44-45 24.69761530734016 25.311160703249485 25.401898584826156 24.5893254045842 46-47 24.689851144729392 25.32937374717975 25.376846084009745 24.603929024081115 48-49 24.816317312537816 25.376564818294845 25.28796926290408 24.51914860626326 50-51 24.578043047384483 25.435024427076076 25.403462487471856 24.58347003806759 52-53 24.912953939209945 25.316069698869896 25.186117168733684 24.58485919318647 54-55 24.675722581734625 25.13548425079499 25.383807908808524 24.804985258661862 56-57 24.6113521868669 25.4985167108925 25.440517707954008 24.449613394286594 58-59 24.760685852246898 25.148900713307732 25.46433090575453 24.626082528690844 60-61 24.81589536839016 25.107341466423645 25.599504365230867 24.477258799955326 62-63 24.75415596234506 25.282998048304822 25.484224125037937 24.478621864312185 64-65 24.63857809011028 25.160223404330623 25.426338245626333 24.77486025993276 66-67 24.5757992592541 25.411694670301554 25.298600690946348 24.713905379497998 68-69 24.417683336916106 25.27585195167089 25.596166429581892 24.71029828183112 70-71 24.375936066404716 25.25925577179851 25.53099804721758 24.83381011457919 72-73 24.606989080748253 25.219762147725156 25.41295124323903 24.760297528287563 74-75 24.523070698442847 25.305569448112703 25.30962669918462 24.86173315425983 76-77 24.676416108046382 25.20218232826641 25.398947051339114 24.722454512348094 78-79 24.51217030116327 25.25765821290365 25.464279280205705 24.765892205727376 80-81 24.42104832622944 25.398589168900948 25.535820410340854 24.644542094528763 82-83 24.571785561593764 25.288385532042557 25.39738792029713 24.742440986066548 84-85 24.507679715500835 25.206027718172507 25.34871329032165 24.937579276005 86-87 24.61676677995547 25.117132828824534 25.588799297905346 24.67730109331465 88-89 24.496342599287836 25.077901730608954 25.521698038048385 24.904057632054823 90-91 24.407064667683038 25.259610951311434 25.658611332141213 24.674713048864312 92-93 24.5327244878771 25.090203385719356 25.645097095721304 24.731975030682246 94-95 24.535950825223658 25.021789196606015 25.524638432005336 24.917621546164987 96-97 24.68939713222538 24.961070627462323 25.469086569838755 24.880445670473545 98-99 24.35675745821742 24.968375536581313 25.53478769692828 25.140079308272988 100-101 24.33455771034266 24.835218433183453 25.834943305336704 24.99528055113718 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 131.0 1 109.5 2 131.5 3 174.0 4 156.0 5 172.5 6 271.0 7 552.5 8 964.0 9 1146.5 10 1067.0 11 1001.0 12 1063.5 13 1163.5 14 1235.0 15 1299.5 16 1332.5 17 1361.5 18 1459.5 19 1574.0 20 1715.0 21 1992.5 22 2369.0 23 2862.5 24 3506.0 25 4537.0 26 6105.5 27 8201.0 28 10999.0 29 14642.5 30 19286.0 31 24725.0 32 30316.5 33 36231.0 34 43330.0 35 50291.5 36 57965.0 37 67518.0 38 77724.5 39 85412.0 40 92004.5 41 103662.5 42 116247.5 43 128877.0 44 141210.5 45 150562.5 46 159681.5 47 170321.5 48 186054.5 49 198540.5 50 204558.5 51 208092.5 52 208079.0 53 209689.5 54 208714.5 55 199506.5 56 188586.0 57 174514.5 58 157493.0 59 140212.5 60 119661.5 61 96989.0 62 76985.5 63 60981.5 64 46857.0 65 35031.0 66 26103.5 67 20138.5 68 14935.5 69 10720.0 70 7992.0 71 5852.0 72 4202.0 73 3154.5 74 2445.5 75 1856.5 76 1295.0 77 782.0 78 472.0 79 310.0 80 192.5 81 146.0 82 130.0 83 107.5 84 90.5 85 75.0 86 68.5 87 61.5 88 59.5 89 49.0 90 36.5 91 36.0 92 34.5 93 33.5 94 33.0 95 29.0 96 27.0 97 22.5 98 15.0 99 12.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06064247318834262 2 0.056980108190306376 3 0.06387793674489 4 0.06342856680648064 5 0.061990583003570694 6 0.012694700760064313 7 0.01417762155681519 8 0.01590769581969121 9 0.006425990119253795 10-11 0.012256565070115192 12-13 0.015008955942872498 14-15 0.017986031784834485 16-17 0.03250068079545669 18-19 0.030961588756404645 20-21 0.030411110581853182 22-23 0.01930043885468185 24-25 0.008032487649067242 26-27 0.01207681709475145 28-29 0.018817366170891792 30-31 0.016817669944970158 32-33 0.019614997811568398 34-35 0.024681643867133892 36-37 0.022906632610416935 38-39 0.010852284012585953 40-41 0.011604978659421625 42-43 0.012380141803177765 44-45 0.02350204777880933 46-47 0.016087443795054952 48-49 0.015244875160537411 50-51 0.015638073856645597 52-53 0.013245178934615775 54-55 0.009785030408863731 56-57 0.013469863903820453 58-59 0.012649763766223378 60-61 0.014042810575292384 62-63 0.021446180310586527 64-65 0.016121146540435654 66-67 0.01880613192243156 68-69 0.025670257731634477 70-71 0.0195812950661877 72-73 0.02582753721007775 74-75 0.04158918779978592 76-77 0.04979018917575667 78-79 0.038432363982460194 80-81 0.019940791016915184 82-83 0.017379382367981852 84-85 0.023187488821922782 86-87 0.025468041259350264 88-89 0.022457262672007577 90-91 0.02654652911153272 92-93 0.05070016330103561 94-95 0.07904417216620577 96-97 0.09351388418298703 98-99 0.09786153833709756 100-101 0.08206618500200868 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4450676.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.52198724434057 #Duplication Level Percentage of deduplicated Percentage of total 1 76.85620401099173 39.59784362702753 2 11.687345815636867 12.043105640668797 3 4.069980018961162 6.290803758649139 4 2.1305210475264165 4.390747129378206 5 1.260974947644527 3.248396758398717 6 0.8112427364112367 2.5078102750466202 7 0.5708604939091729 2.058830695984046 8 0.41176322155697725 1.6971887558997731 9 0.3099380248701813 1.4371760667507831 >10 1.724795353092163 16.00092412273377 >50 0.10720088345156165 3.7900732556349634 >100 0.055479453479297794 5.1760340224105095 >500 0.002506637989128 0.8774516481469571 >1k 0.0011873544795733544 0.8836142432703026 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2468496920467813E-5 2 0.0 0.0 0.0 0.0 2.2468496920467813E-5 3 0.0 0.0 0.0 0.0 2.2468496920467813E-5 4 0.0 0.0 0.0 0.0 2.2468496920467813E-5 5 0.0 2.2468496920467813E-5 0.0 0.0 2.2468496920467813E-5 6 2.2468496920467813E-5 2.2468496920467813E-5 0.0 0.0 2.2468496920467813E-5 7 2.2468496920467813E-5 2.2468496920467813E-5 0.0 0.0 4.4936993840935626E-5 8 2.2468496920467813E-5 2.2468496920467813E-5 0.0 0.0 4.4936993840935626E-5 9 2.2468496920467813E-5 2.2468496920467813E-5 0.0 0.0 4.4936993840935626E-5 10-11 2.2468496920467813E-5 2.2468496920467813E-5 0.0 0.0 4.4936993840935626E-5 12-13 2.2468496920467813E-5 2.2468496920467813E-5 0.0 0.0 6.740549076140344E-5 14-15 6.740549076140344E-5 3.370274538070172E-5 0.0 1.1234248460233906E-5 8.987398768187125E-5 16-17 6.740549076140344E-5 4.4936993840935626E-5 0.0 3.370274538070172E-5 8.987398768187125E-5 18-19 7.863973922163734E-5 4.4936993840935626E-5 0.0 4.4936993840935626E-5 1.3481098152280687E-4 20-21 8.987398768187125E-5 4.4936993840935626E-5 0.0 6.740549076140344E-5 1.685137269035086E-4 22-23 8.987398768187125E-5 4.4936993840935626E-5 0.0 6.740549076140344E-5 2.022164722842103E-4 24-25 2.1345072074444423E-4 4.4936993840935626E-5 0.0 1.1234248460233905E-4 2.246849692046781E-4 26-27 2.246849692046781E-4 4.4936993840935626E-5 0.0 1.909822238239764E-4 2.246849692046781E-4 28-29 2.583877145853798E-4 4.4936993840935626E-5 0.0 8.088658891368413E-4 2.246849692046781E-4 30-31 2.6962196304561374E-4 4.4936993840935626E-5 0.0 0.002763625121217541 2.246849692046781E-4 32-33 2.6962196304561374E-4 4.4936993840935626E-5 0.0 0.00765052320141929 2.246849692046781E-4 34-35 3.0332470842631544E-4 4.4936993840935626E-5 0.0 0.013818125606087705 2.246849692046781E-4 36-37 3.370274538070172E-4 5.617124230116953E-5 0.0 0.024726580860974824 2.246849692046781E-4 38-39 3.370274538070172E-4 7.863973922163734E-5 0.0 0.042308179701240886 2.246849692046781E-4 40-41 3.370274538070172E-4 8.987398768187125E-5 0.0 0.0614625733259397 2.471534661251459E-4 42-43 3.59495950727485E-4 8.987398768187125E-5 0.0 0.08244814944965664 2.471534661251459E-4 44-45 4.2690144148888845E-4 8.987398768187125E-5 0.0 0.10727583854677357 2.583877145853798E-4 46-47 4.8307268379005797E-4 8.987398768187125E-5 0.0 0.13547380218196067 2.808562115058477E-4 48-49 5.392439260912275E-4 8.987398768187125E-5 0.0 0.1665589676714279 3.145589568865494E-4 50-51 5.392439260912275E-4 8.987398768187125E-5 1.1234248460233906E-5 0.19777894414241792 3.257932053467833E-4 52-53 5.617124230116954E-4 8.987398768187125E-5 2.2468496920467813E-5 0.23273992535066584 3.59495950727485E-4 54-55 5.617124230116954E-4 8.987398768187125E-5 2.2468496920467813E-5 0.2726552101298769 3.59495950727485E-4 56-57 5.617124230116954E-4 8.987398768187125E-5 2.2468496920467813E-5 0.32217577734258795 3.59495950727485E-4 58-59 5.617124230116954E-4 8.987398768187125E-5 2.2468496920467813E-5 0.3756732685102218 3.59495950727485E-4 60-61 5.729466714719292E-4 8.987398768187125E-5 2.2468496920467813E-5 0.4304402297538621 3.59495950727485E-4 62-63 5.841809199321631E-4 8.987398768187125E-5 2.2468496920467813E-5 0.49180169484365965 3.819644476479528E-4 64-65 5.841809199321631E-4 8.987398768187125E-5 2.2468496920467813E-5 0.5615326750363316 4.0443294456842064E-4 66-67 6.515864106935665E-4 8.987398768187125E-5 2.2468496920467813E-5 0.6447559876297444 4.0443294456842064E-4 68-69 6.740549076140344E-4 8.987398768187125E-5 2.2468496920467813E-5 0.7386518362603793 4.0443294456842064E-4 70-71 7.302261499152039E-4 8.987398768187125E-5 2.2468496920467813E-5 0.8424899947783213 4.0443294456842064E-4 72-73 7.751631437561395E-4 8.987398768187125E-5 2.2468496920467813E-5 0.9543381724484101 4.156671930286546E-4 74-75 7.863973922163734E-4 8.987398768187125E-5 2.2468496920467813E-5 1.0829927858150088 4.2690144148888845E-4 76-77 7.863973922163734E-4 8.987398768187125E-5 2.2468496920467813E-5 1.2301951433894538 4.2690144148888845E-4 78-79 7.863973922163734E-4 8.987398768187125E-5 2.2468496920467813E-5 1.3958553711840629 4.2690144148888845E-4 80-81 9.212083737391803E-4 8.987398768187125E-5 2.2468496920467813E-5 1.5784006744144037 4.2690144148888845E-4 82-83 9.436768706596481E-4 8.987398768187125E-5 2.2468496920467813E-5 1.774719166256991 4.2690144148888845E-4 84-85 9.436768706596481E-4 8.987398768187125E-5 2.2468496920467813E-5 1.9916749725210283 4.2690144148888845E-4 86-87 9.773796160403498E-4 8.987398768187125E-5 2.2468496920467813E-5 2.233042800689154 4.2690144148888845E-4 88-89 9.886138645005837E-4 8.987398768187125E-5 2.2468496920467813E-5 2.5020918170632958 4.2690144148888845E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3510 0.0 41.154255 1 GTATCAA 5770 0.0 38.128826 1 ATCAACG 6700 0.0 33.828064 3 TCAACGC 6760 0.0 33.385365 4 CAACGCA 6980 0.0 32.33165 5 AACGCAG 7435 0.0 30.468517 6 TATCAAC 7160 0.0 29.93035 2 ACGCAGA 8860 0.0 25.621996 7 GTACATG 11940 0.0 25.429907 1 CGCAGAG 9380 0.0 24.099783 8 ACATGGG 12860 0.0 23.351227 3 TACATGG 12620 0.0 23.344286 2 CATGGGG 10990 0.0 21.529842 4 GCAGAGT 11580 0.0 19.562912 9 AGAGTAC 10110 0.0 17.523285 10-11 ATGGGGA 6660 0.0 17.477587 5 CAGAGTA 10455 0.0 17.263046 10-11 GTATAGA 925 0.0 15.924588 1 GTGGTAT 1970 0.0 15.919374 1 AGTACAT 8670 0.0 15.510135 2 >>END_MODULE