FastQCFastQC Report
Fri 27 May 2016
SRR940211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4450676
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34350.047.0338861
GTATCAA58700.039.7467881
TCAACGC72550.030.9069294
ATCAACG72800.030.6706283
CAACGCA75800.029.6437725
TATCAAC78400.028.904362
AACGCAG80250.028.0553876
GTACATG119500.026.0056971
ACGCAGA93550.024.0664847
TACATGG125650.024.0467112
ACATGGG126800.023.11653
CGCAGAG99750.022.4751368
CATGGGG108150.020.6453694
GCAGAGT122200.018.2683739
AGAGTAC105100.018.12236410-11
CCTATAA21600.017.379291
ATGGGGA71250.017.2685725
TATAAGC21050.017.1521153
GAGTACA89450.017.1054381
TTATAGG22200.016.9055883
Produced by FastQC (version 0.11.5)