FastQCFastQC Report
Fri 27 May 2016
SRR940210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4535444
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60150.041.3392871
GGTATCA34500.038.819861
ATCAACG73350.035.93353
TCAACGC73750.035.6738174
CAACGCA77000.033.9827045
AACGCAG79750.032.8689846
TATCAAC77800.031.09642
ACGCAGA93950.027.9025637
CGCAGAG100650.025.8101048
GTACATG124800.024.5015561
ACATGGG134350.023.4005973
TACATGG131050.022.9662272
CATGGGG111700.021.3003334
GCAGAGT124450.020.5291069
AGAGTAC105850.019.24659310-11
TATAAGC22850.017.873883
CAGAGTA108850.017.47276110-11
ATTCGCG6000.017.0161180-81
ATGGGGA70850.016.5559525
GAGTACA92850.015.561571
Produced by FastQC (version 0.11.5)