##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940210_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4535444 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.517317157923237 33.0 31.0 34.0 28.0 34.0 2 29.714127216651775 33.0 31.0 34.0 16.0 34.0 3 31.077159149137326 33.0 31.0 34.0 25.0 34.0 4 35.15127206950411 37.0 35.0 37.0 32.0 37.0 5 35.46143861549167 37.0 35.0 37.0 33.0 37.0 6 35.694763291091235 37.0 35.0 37.0 35.0 37.0 7 35.75184965352896 37.0 37.0 37.0 35.0 37.0 8 35.78780092974359 37.0 37.0 37.0 35.0 37.0 9 37.56395316533508 39.0 38.0 39.0 35.0 39.0 10-11 37.5000011024279 39.0 38.0 39.0 35.0 39.0 12-13 37.428819096873426 39.0 38.0 39.0 35.0 39.0 14-15 38.84939655301664 41.0 39.0 41.0 36.0 41.0 16-17 38.78579814897946 41.0 39.0 41.0 36.0 41.0 18-19 38.72054610309377 41.0 39.0 41.0 35.0 41.0 20-21 38.631446777867836 41.0 39.0 41.0 35.0 41.0 22-23 38.5230409635749 40.0 39.0 41.0 34.5 41.0 24-25 38.40921440105974 40.0 38.5 41.0 34.0 41.0 26-27 38.273016930646705 40.0 38.0 41.0 34.0 41.0 28-29 38.111138292083425 40.0 38.0 41.0 34.0 41.0 30-31 37.95469550500458 40.0 38.0 41.0 33.0 41.0 32-33 38.033205679532145 40.0 38.0 41.0 34.0 41.0 34-35 38.08005136873038 40.0 38.0 41.0 33.5 41.0 36-37 38.182795995276315 40.0 38.0 41.0 34.0 41.0 38-39 38.11572946331164 40.0 38.0 41.0 34.0 41.0 40-41 37.98907923898961 40.0 38.0 41.0 33.0 41.0 42-43 37.743185341942265 40.0 38.0 41.0 33.0 41.0 44-45 37.69525023349423 40.0 38.0 41.0 33.0 41.0 46-47 37.51792900540719 40.0 38.0 41.0 33.0 41.0 48-49 37.326941750355644 40.0 37.0 41.0 32.0 41.0 50-51 36.41212988188147 39.0 36.0 40.5 31.0 40.5 52-53 36.505180529183036 39.0 36.0 40.5 31.0 41.0 54-55 36.80984573946894 40.0 36.0 41.0 31.0 41.0 56-57 36.64162780975799 39.5 36.0 41.0 31.0 41.0 58-59 36.37732832772271 39.0 35.0 41.0 31.0 41.0 60-61 36.06278856050257 39.0 35.0 41.0 30.5 41.0 62-63 35.71085223409219 38.0 35.0 40.5 30.0 41.0 64-65 35.32280775597714 37.0 35.0 40.0 29.0 41.0 66-67 34.94484024055859 37.0 35.0 39.5 29.0 41.0 68-69 34.55859349161846 36.0 34.5 39.0 29.0 41.0 70-71 34.140614347790425 36.0 34.0 39.0 28.5 40.5 72-73 33.71949692246228 35.0 34.0 37.5 28.0 40.0 74-75 33.29257477327468 35.0 34.0 37.0 27.5 39.0 76-77 32.839964731126656 35.0 33.5 36.5 26.0 39.0 78-79 32.41799523927536 35.0 33.0 36.0 26.0 38.0 80-81 32.09174680582541 35.0 33.0 36.0 25.5 37.0 82-83 31.759985791909237 35.0 33.0 35.0 25.0 37.0 84-85 31.478870095188036 35.0 33.0 35.0 24.0 36.0 86-87 31.211435638936344 35.0 32.0 35.0 23.5 36.0 88-89 30.93677267319363 35.0 32.0 35.0 21.0 36.0 90-91 30.70102188451671 34.5 32.0 35.0 20.0 35.0 92-93 30.439718911753733 34.0 31.5 35.0 18.5 35.0 94-95 30.125629155601963 34.0 31.0 35.0 13.0 35.0 96-97 29.78549024086727 34.0 31.0 35.0 2.0 35.0 98-99 29.359726412673158 34.0 31.0 35.0 2.0 35.0 100-101 27.487027510426763 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6058324007379419 1101 2 1.7610310548292745 1101 3 0.9533387405923115 1101 4 0.4843185023229566 1101 5 0.38884060036799184 1101 6 0.3177360338842803 1101 7 0.24453221220593235 1101 8 0.22278329330077895 1101 9 0.32353210746907735 1101 10-11 0.29368135016026997 1101 12-13 0.31367842620452535 1101 14-15 0.34591937542993634 1101 16-17 0.4109943914061276 1101 18-19 0.3720995774802063 1101 20-21 0.37412158437390275 1101 22-23 0.4064823073171482 1101 24-25 0.4189559904580378 1101 26-27 0.4212426239749618 1101 28-29 0.4917526921008033 1101 30-31 0.49938733309067374 1101 32-33 0.47113881841205796 1101 34-35 0.5355538404693476 1101 36-37 0.5697549165405462 1101 38-39 0.6111931873659486 1101 40-41 0.6024883086090682 1101 42-43 0.6814097167069235 1101 44-45 0.6019756924847144 1101 46-47 0.5325886455263671 1101 48-49 0.5613393390558272 1101 50-51 0.5115911753783777 1101 52-53 0.5625513350687754 1101 54-55 0.7147706455294198 1101 56-57 0.7630794856537548 1101 58-59 0.6893201974868148 1101 60-61 0.7786605652787415 1101 62-63 0.8557001653793321 1101 64-65 0.6951194911496614 1101 66-67 0.7464018908146457 1101 68-69 0.7583731167073395 1101 70-71 0.703200425898423 1101 72-73 0.6974001289482743 1101 74-75 0.7415863041045583 1101 76-77 0.766186610912662 1101 78-79 0.8290797842875506 1101 80-81 0.7524546950304298 1101 82-83 0.7669955467209952 1101 84-85 0.7311165770506385 1101 86-87 0.6334908215605815 1101 88-89 0.5888738206121822 1101 90-91 0.6368892248032125 1101 92-93 0.5421550307407799 1101 94-95 0.42135146395053624 1101 96-97 0.20379697058875124 1101 98-99 0.2318394545177398 1101 100-101 0.14193133564143423 1102 1 0.9574210150158287 1102 2 2.7256453551773454 1102 3 1.5117767187100348 1102 4 0.8493359299119021 1102 5 0.611287817604854 1102 6 0.48679505638614273 1102 7 0.3910890119046684 1102 8 0.39589682579313035 1102 9 0.3908048469063772 1102 10-11 0.3471591200979347 1102 12-13 0.41399103440265606 1102 14-15 0.41829261472015844 1102 16-17 0.4164672899342392 1102 18-19 0.4792056538748639 1102 20-21 0.48828868970716854 1102 22-23 0.5353490648154775 1102 24-25 0.6034869413637765 1102 26-27 0.5655067285897246 1102 28-29 0.5583600541490128 1102 30-31 0.6148436761858633 1102 32-33 0.5826812717415635 1102 34-35 0.6316578179548173 1102 36-37 0.6039807980214817 1102 38-39 0.6033120928173119 1102 40-41 0.5937382838183325 1102 42-43 0.7523038152213672 1102 44-45 0.5528850465611796 1102 46-47 0.45595558115957857 1102 48-49 0.5093080637797271 1102 50-51 0.4150439514749067 1102 52-53 0.5343658508991282 1102 54-55 0.6709647274848436 1102 56-57 0.7308127294457378 1102 58-59 0.7063886791778629 1102 60-61 0.7188638457323435 1102 62-63 0.8217950103159666 1102 64-65 0.82037755375422 1102 66-67 0.7003883128984967 1102 68-69 0.665340382899636 1102 70-71 0.6433707018175241 1102 72-73 0.6265725472648924 1102 74-75 0.6901226769534077 1102 76-77 0.7223612186993194 1102 78-79 0.6257515114503818 1102 80-81 0.5715525702910824 1102 82-83 0.6100286090806328 1102 84-85 0.6040256725866122 1102 86-87 0.5611682390038482 1102 88-89 0.6487649839034475 1102 90-91 0.5733485067057842 1102 92-93 0.43233353240540495 1102 94-95 0.4332697448426046 1102 96-97 0.4796621015758298 1102 98-99 0.5523367817776794 1102 100-101 0.578110303013478 1103 1 0.8995150315404068 1103 2 2.7167765631097147 1103 3 1.4250491042207791 1103 4 0.8869184217815302 1103 5 0.5691093660046747 1103 6 0.4766721747027418 1103 7 0.38673112266607745 1103 8 0.38958751359476906 1103 9 0.3800874552351132 1103 10-11 0.41133866078178727 1103 12-13 0.3486985363769506 1103 14-15 0.4560056900312759 1103 16-17 0.49066033756520966 1103 18-19 0.4342809525511697 1103 20-21 0.48756015402724984 1103 22-23 0.40644932802550215 1103 24-25 0.49470771443552053 1103 26-27 0.5209881017945293 1103 28-29 0.4968077279427021 1103 30-31 0.581958634918827 1103 32-33 0.5859579611759358 1103 34-35 0.5460564856483856 1103 36-37 0.5450026090095008 1103 38-39 0.5172732615657551 1103 40-41 0.5807312403073936 1103 42-43 0.6766313771219146 1103 44-45 0.5167861646930021 1103 46-47 0.5137435287520944 1103 48-49 0.5475423624533775 1103 50-51 0.4257789985604532 1103 52-53 0.49846417667727394 1103 54-55 0.594809041819623 1103 56-57 0.7190758764000762 1103 58-59 0.6943961002259655 1103 60-61 0.6353661726937361 1103 62-63 0.5840342098661253 1103 64-65 0.5459656678184075 1103 66-67 0.5780221897965063 1103 68-69 0.44191855485582465 1103 70-71 0.45756745375428665 1103 72-73 0.5235077894261124 1103 74-75 0.5678407224302049 1103 76-77 0.5990198282788128 1103 78-79 0.6521852199224085 1103 80-81 0.6064768674279364 1103 82-83 0.6969870764396582 1103 84-85 0.6119758279098413 1103 86-87 0.5934135768640729 1103 88-89 0.5216563278202564 1103 90-91 0.5214213793727147 1103 92-93 0.44488009162055064 1103 94-95 0.3933921601565764 1103 96-97 0.2671972902600217 1103 98-99 0.16807657250661023 1103 100-101 0.12409499543617741 1104 1 0.8256900304943109 1104 2 2.5658633999896274 1104 3 1.4273153500347924 1104 4 0.8227094897490588 1104 5 0.4901160217402136 1104 6 0.3519010748045872 1104 7 0.2702570448342172 1104 8 0.16707526137306417 1104 9 0.26475210822794537 1104 10-11 0.24821366374751364 1104 12-13 0.2556704074931986 1104 14-15 0.26481462870696504 1104 16-17 0.2526296290393262 1104 18-19 0.31391799949074084 1104 20-21 0.2939054913240895 1104 22-23 0.35132425204899675 1104 24-25 0.2791149316530337 1104 26-27 0.30337495881980914 1104 28-29 0.37900721581039676 1104 30-31 0.299544601061676 1104 32-33 0.3707710514362361 1104 34-35 0.40674793758572747 1104 36-37 0.41492161989360454 1104 38-39 0.48206696999976373 1104 40-41 0.42186081944179676 1104 42-43 0.5430630720373912 1104 44-45 0.4869627191970025 1104 46-47 0.3503635871520814 1104 48-49 0.3900895045506729 1104 50-51 0.3181863525248829 1104 52-53 0.41349182155459374 1104 54-55 0.38905847811604843 1104 56-57 0.4702111561985092 1104 58-59 0.5063383011438489 1104 60-61 0.5124298864035026 1104 62-63 0.617342329138765 1104 64-65 0.5784077951863011 1104 66-67 0.4729186503808265 1104 68-69 0.5264395265267794 1104 70-71 0.3545813346446991 1104 72-73 0.38238815356114486 1104 74-75 0.44079766095578066 1104 76-77 0.587021742304259 1104 78-79 0.6193327439620404 1104 80-81 0.6023692294871736 1104 82-83 0.6185960980262557 1104 84-85 0.6125586199712707 1104 86-87 0.6278439806643448 1104 88-89 0.6834193018456745 1104 90-91 0.49370850605255967 1104 92-93 0.2692803356081086 1104 94-95 0.25512193962305574 1104 96-97 0.20799293946492625 1104 98-99 0.19309036582723493 1104 100-101 0.12376919698732891 1105 1 0.693923567792023 1105 2 2.3296199446314283 1105 3 1.2038859546032121 1105 4 0.6508830741558853 1105 5 0.38745779888034093 1105 6 0.2973049332320272 1105 7 0.21408368394237698 1105 8 0.1602511737134904 1105 9 0.08780184093780008 1105 10-11 0.12120080392122645 1105 12-13 0.12254695653857794 1105 14-15 -0.03472307891750148 1105 16-17 0.037478993074550715 1105 18-19 0.07013351964576486 1105 20-21 0.12256228093581001 1105 22-23 0.13871948721825333 1105 24-25 0.08528099494738939 1105 26-27 0.07940616668270195 1105 28-29 0.12042696131606334 1105 30-31 0.1533411448778068 1105 32-33 0.18404184713915583 1105 34-35 0.13813233978426354 1105 36-37 0.2881448373459534 1105 38-39 0.03523335584792875 1105 40-41 0.15844502047684728 1105 42-43 0.17935336754630526 1105 44-45 0.0342282155931386 1105 46-47 0.009617336918303465 1105 48-49 -0.01954382453615011 1105 50-51 -0.01912674297210515 1105 52-53 0.07303731884311304 1105 54-55 0.12993627275706388 1105 56-57 0.11588590144459232 1105 58-59 0.14468844779428736 1105 60-61 0.1716037274772546 1105 62-63 0.2421669522334895 1105 64-65 0.30962529090521684 1105 66-67 0.09925835896482482 1105 68-69 0.08655119227847052 1105 70-71 0.05741013363321201 1105 72-73 0.09116777841875745 1105 74-75 0.10496217257240659 1105 76-77 0.11398657351396224 1105 78-79 0.18703182607023194 1105 80-81 0.19673639121590725 1105 82-83 0.25181191571475736 1105 84-85 0.23531572944711598 1105 86-87 0.26624208295539376 1105 88-89 0.17026194208308354 1105 90-91 -0.07736420324722815 1105 92-93 0.048830861983816476 1105 94-95 -0.26534406827211043 1105 96-97 -0.3315239679216795 1105 98-99 -0.3509263965456846 1105 100-101 -0.2046494303179074 1106 1 0.7494354126982081 1106 2 2.5015512633049006 1106 3 1.2980355323093953 1106 4 0.7859328696267553 1106 5 0.46972188679544047 1106 6 0.3445536299680896 1106 7 0.18944474546263734 1106 8 0.15665809715405032 1106 9 0.12440274585021172 1106 10-11 0.16427183664955436 1106 12-13 0.16669574908457463 1106 14-15 0.18615762404763103 1106 16-17 0.13933491801568465 1106 18-19 0.0645976304834619 1106 20-21 0.16661868969877247 1106 22-23 0.17694200283885664 1106 24-25 0.18202654676000662 1106 26-27 0.08731920478764721 1106 28-29 0.1349461027056762 1106 30-31 0.07400687710187981 1106 32-33 0.22302515239027088 1106 34-35 0.1791335577002755 1106 36-37 0.11819941311676985 1106 38-39 0.15133417694193696 1106 40-41 0.2818235627219323 1106 42-43 0.2011818914166028 1106 44-45 0.23213171553134515 1106 46-47 0.29519676882125623 1106 48-49 0.035682762496918485 1106 50-51 0.08871883253807766 1106 52-53 0.1676573808483326 1106 54-55 0.3935600766114078 1106 56-57 0.3940259405761637 1106 58-59 0.2947952703672527 1106 60-61 0.34805196054053766 1106 62-63 0.3368009329874795 1106 64-65 0.2425675634810247 1106 66-67 0.2907146230951554 1106 68-69 0.33363977751770335 1106 70-71 0.20768612253737473 1106 72-73 0.2730543164496808 1106 74-75 0.09255990462190766 1106 76-77 0.2599255210893219 1106 78-79 0.2795440388800472 1106 80-81 0.27895862091585144 1106 82-83 0.28887468855792164 1106 84-85 0.09308375764272725 1106 86-87 0.09495342299338105 1106 88-89 0.19692220918438252 1106 90-91 0.08574492300612135 1106 92-93 0.12255668975678802 1106 94-95 0.05377919127248276 1106 96-97 0.139070763388343 1106 98-99 0.14810698968909364 1106 100-101 -0.10644648863879169 1107 1 0.7360673753376084 1107 2 2.4612017327421505 1107 3 1.2932473348012898 1107 4 0.6586663592082331 1107 5 0.4657922148521294 1107 6 0.24858709977947058 1107 7 0.12423798210011228 1107 8 0.08493213094197927 1107 9 0.10780679678718741 1107 10-11 0.13033089985160018 1107 12-13 0.14351494894811623 1107 14-15 0.06436577541511923 1107 16-17 0.09875828997389391 1107 18-19 0.10180936622933245 1107 20-21 0.12470831223441081 1107 22-23 0.14568710544720176 1107 24-25 0.09463456260575498 1107 26-27 0.21019518834001616 1107 28-29 0.18530587741638982 1107 30-31 0.1215650998310096 1107 32-33 0.1279657435531334 1107 34-35 0.1207256913109731 1107 36-37 0.09237804673986005 1107 38-39 0.08517330654260746 1107 40-41 0.03501902844229221 1107 42-43 0.06716981253373433 1107 44-45 -0.0029587458183186754 1107 46-47 -0.09130838125873453 1107 48-49 -0.09100150111395067 1107 50-51 -0.12996296381201233 1107 52-53 0.006296046974007652 1107 54-55 0.12275173644246706 1107 56-57 0.11908064011075226 1107 58-59 0.19613513817500206 1107 60-61 0.16123170386500618 1107 62-63 0.2070333740514272 1107 64-65 0.14475461295757697 1107 66-67 0.18061908748664735 1107 68-69 0.1276228189542934 1107 70-71 0.10992940358436698 1107 72-73 0.11256043688408823 1107 74-75 0.17297973513776554 1107 76-77 0.15735030544795592 1107 78-79 0.18316398099055675 1107 80-81 0.2040506796812238 1107 82-83 0.2576326963726707 1107 84-85 0.22840009181858534 1107 86-87 0.18323584199406184 1107 88-89 0.12282934517315525 1107 90-91 0.12162600755478792 1107 92-93 0.02670945344211262 1107 94-95 -0.12171205065432389 1107 96-97 0.018061692369993665 1107 98-99 0.12950268312657087 1107 100-101 0.251378096574701 1108 1 0.7370059016091837 1108 2 2.529068822994045 1108 3 1.2887910331185175 1108 4 0.6933705639277221 1108 5 0.37616672688639596 1108 6 0.31821150845170365 1108 7 0.18600814148983602 1108 8 0.17541421443563365 1108 9 0.1714185475577068 1108 10-11 0.17549916892246387 1108 12-13 0.1596527524303184 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sequence quality scores pass #Quality Count 2 32312.0 3 8612.0 4 7300.0 5 6002.0 6 5529.0 7 5344.0 8 5699.0 9 6464.0 10 7212.0 11 8299.0 12 9288.0 13 10275.0 14 10976.0 15 12211.0 16 14354.0 17 17151.0 18 16012.0 19 13986.0 20 14395.0 21 15690.0 22 16679.0 23 18221.0 24 20549.0 25 24165.0 26 28293.0 27 34164.0 28 40918.0 29 50470.0 30 62259.0 31 78448.0 32 101203.0 33 136575.0 34 199984.0 35 321354.0 36 616423.0 37 1220954.0 38 1188289.0 39 149376.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.613522261027065 17.868330260854144 13.454767945109156 25.063379533009634 2 17.713175987413756 20.442975424760192 36.69973521529558 25.144113372530473 3 18.870263136536707 23.220202619014852 28.687885733038332 29.22164851141011 4 13.735158927095714 16.033155695158687 35.19681755435395 35.03486782339165 5 15.569538870417238 35.7917721204903 33.50080227614841 15.13788673294405 6 34.40216649470339 33.93381924076101 17.273477617834903 14.390536646700697 7 30.352318822490084 30.477887066206264 20.66720093080162 18.502593180502036 8 27.37192006548885 33.725238790855556 19.789935499123168 19.11290564453242 9 26.97734263883835 15.9193155382088 19.276993131126034 37.82634869182681 10-11 26.070893754188322 25.10892678096031 27.009255055263292 21.81092440958807 12-13 26.623326356833626 22.192127950033854 27.204346013801562 23.980199679330962 14-15 23.91371558325084 23.630281909883838 24.39806454472543 28.057937962139896 16-17 24.472700863285205 26.642459481746577 24.993356036391056 23.891483618577162 18-19 24.47741238669912 25.937160792108426 26.128426761311562 23.45700005988089 20-21 24.842560896912364 25.35560629233628 25.965046914365637 23.83678589638572 22-23 24.842977275471704 25.005748264079386 25.831800003418632 24.319474457030278 24-25 24.780548342209304 25.23600624609082 25.67971110892809 24.303734302771787 26-27 24.520408488421054 25.545819437303553 25.635802972113925 24.297969102161467 28-29 24.496938731739657 25.529730134451444 25.768355011183058 24.20497612262584 30-31 24.564347937693043 25.30210061258127 25.664857312096238 24.46869413762944 32-33 24.488207714664938 25.35065656300548 25.631728783833317 24.529406938496265 34-35 24.861861759868166 25.367218131606144 25.435741403273678 24.335178705252012 36-37 24.782994692540388 25.23790719956879 25.438920436845684 24.54017767104513 38-39 24.565722652589148 25.37014128236848 25.7414402554417 24.322695809600674 40-41 24.734104162874246 25.1301955990894 25.73555703473011 24.400143203306246 42-43 24.86309546910723 25.16100643187784 25.432213458607357 24.543684640407577 44-45 24.627699398510334 25.362255558679696 25.41008804573747 24.599956997072493 46-47 24.71916405811547 25.31867693254226 25.347818464683396 24.614340544658873 48-49 24.8338900468098 25.350434007181562 25.2901687075574 24.525507238451247 50-51 24.572705320775082 25.431848872247276 25.394444943114493 24.601000863863153 52-53 24.88068158638415 25.296286536199077 25.207493745472426 24.615538131944344 54-55 24.71402302414429 25.14123119531512 25.35392562354806 24.790820156992524 56-57 24.597730300009705 25.53822675828974 25.4488126637439 24.415230277956653 58-59 24.753159781943012 25.155439151854566 25.45096816323002 24.640432902972403 60-61 24.8233735008042 25.13727938020211 25.55980469891818 24.47954242007551 62-63 24.730198781049513 25.25425861329964 25.51346009498026 24.502082510670586 64-65 24.65095595396037 25.155804529159376 25.41636086490148 24.776878651978773 66-67 24.5624289302946 25.415370142437666 25.32024851366253 24.701952413605206 68-69 24.385105252462683 25.261748773000082 25.606465376897614 24.74668059763962 70-71 24.407348539603728 25.26080060335517 25.47897656761255 24.85287428942855 72-73 24.606901646846072 25.218721227057454 25.399152042430963 24.775225083665514 74-75 24.504076756697387 25.31732184355991 25.311466240740483 24.867135159002224 76-77 24.665965192099787 25.163179951383448 25.40685818936638 24.763996667150394 78-79 24.515480860394046 25.259359629105973 25.44130448062905 24.78385502987093 80-81 24.39185967685523 25.40587161450213 25.54022858216516 24.662040126477486 82-83 24.56250980644679 25.308246324491368 25.365891416625935 24.763352452435907 84-85 24.52059161225111 25.214210081957155 25.321186942560086 24.94401136323165 86-87 24.65111893906856 25.117319199636988 25.58870215859388 24.642859702700573 88-89 24.49929873649066 25.081922508480055 25.522088174058467 24.89669058097082 90-91 24.423358712147483 25.238212380857973 25.646642620948708 24.691786286045836 92-93 24.53739225358458 25.100496413516836 25.629466698738174 24.732644634160412 94-95 24.529186021243245 24.99700511942569 25.56184069882753 24.911968160503537 96-97 24.662031150448335 24.9746719880407 25.47775696913364 24.885539892377327 98-99 24.331277093651767 24.988497782857998 25.524892783889612 25.155332339600616 100-101 24.28269840170125 24.89940854252929 25.817639315527973 25.00025374024148 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 210.0 1 154.5 2 145.0 3 186.0 4 166.0 5 184.0 6 269.5 7 546.5 8 952.5 9 1140.5 10 1053.5 11 1019.0 12 1101.0 13 1189.0 14 1311.0 15 1342.5 16 1353.5 17 1394.0 18 1505.0 19 1652.0 20 1769.0 21 2022.0 22 2416.0 23 2891.0 24 3535.0 25 4659.5 26 6313.0 27 8467.0 28 11203.0 29 14654.0 30 19474.0 31 25047.5 32 30681.5 33 36969.5 34 44286.5 35 51124.5 36 58954.0 37 68803.0 38 79203.0 39 86825.5 40 93573.5 41 105972.5 42 119085.5 43 132388.0 44 144603.0 45 153741.5 46 163190.0 47 174150.5 48 189830.5 49 202430.5 50 208009.5 51 211171.0 52 211581.5 53 213144.5 54 212705.5 55 203862.5 56 191783.0 57 177237.5 58 160181.0 59 142189.0 60 121488.5 61 98950.0 62 78762.5 63 61957.5 64 47844.5 65 36101.5 66 26730.5 67 20576.5 68 15318.5 69 10935.0 70 7984.0 71 5888.5 72 4335.0 73 3230.5 74 2457.0 75 1841.5 76 1308.0 77 822.0 78 478.0 79 307.5 80 223.5 81 170.0 82 127.0 83 103.5 84 76.0 85 66.5 86 67.5 87 55.5 88 46.5 89 39.0 90 37.5 91 38.5 92 30.5 93 21.5 94 18.0 95 18.0 96 20.0 97 20.0 98 17.5 99 18.5 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03629192643542727 2 1.1005096744662706 3 0.02689924073585739 4 0.029126145091858704 5 0.03238933167292993 6 0.012369241026898358 7 0.01891766274702102 8 0.02054925603755663 9 0.010891987642224222 10-11 0.011399104475769075 12-13 0.025885007068767688 14-15 0.015367844912207052 16-17 0.027626843149204355 18-19 0.031827093444434545 20-21 0.044637305630937124 22-23 0.0323342102779794 24-25 0.009866729696144414 26-27 0.015996228814643063 28-29 0.02492589479662851 30-31 0.019005856978941863 32-33 0.03038291289673073 34-35 0.02685514361989697 36-37 0.025697594325935897 38-39 0.012214901121036882 40-41 0.013945712922483445 42-43 0.01172983284547224 44-45 0.019160196884803343 46-47 0.014970970868563254 48-49 0.010936084758184644 50-51 0.025598375815024943 52-53 0.016073398767573802 54-55 0.016966365365772347 56-57 0.020372867573714946 58-59 0.02277616039355794 60-61 0.017738064895079734 62-63 0.024992040470569143 64-65 0.020196479109873256 66-67 0.01595213169868264 68-69 0.015533209097058635 70-71 0.0176719192211391 72-73 0.022467480581834984 74-75 0.029214339323779547 76-77 0.02614958976453022 78-79 0.02431955945217271 80-81 0.019446828138546084 82-83 0.017627822105178676 84-85 0.018046744706802686 86-87 0.024837700564707663 88-89 0.02488179768066809 90-91 0.019568095207437245 92-93 0.04022759403489493 94-95 0.059729543568391545 96-97 0.0751084127595887 98-99 0.08177810154860252 100-101 0.07165781343568567 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4535444.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.121997027732206 #Duplication Level Percentage of deduplicated Percentage of total 1 77.14391965977634 40.20895151214475 2 11.524272669156923 12.013362116171454 3 4.1486102498121875 6.487015533297905 4 2.0604675168060527 4.295827271468154 5 1.1890285352153864 3.0987270889192575 6 0.7868314844777612 2.460673697716559 7 0.5598630720622539 2.0426826964576072 8 0.4279409197283458 1.7844108276900663 9 0.31205938106815084 1.4638642319259099 >10 1.687026690078281 15.832615206976557 >50 0.10360851979799887 3.6874360306997302 >100 0.05284808468192711 4.956493797781481 >500 0.002534998091702545 0.9140426031357674 >1k 9.88219246674115E-4 0.7538973856147427 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.204855798021098E-5 2 0.0 0.0 0.0 0.0 2.204855798021098E-5 3 0.0 0.0 0.0 0.0 2.204855798021098E-5 4 0.0 0.0 0.0 0.0 2.204855798021098E-5 5 0.0 0.0 0.0 2.204855798021098E-5 2.204855798021098E-5 6 0.0 0.0 0.0 2.204855798021098E-5 2.204855798021098E-5 7 0.0 0.0 0.0 2.204855798021098E-5 2.204855798021098E-5 8 0.0 0.0 0.0 2.204855798021098E-5 2.204855798021098E-5 9 0.0 0.0 0.0 2.204855798021098E-5 2.204855798021098E-5 10-11 0.0 0.0 0.0 2.204855798021098E-5 2.204855798021098E-5 12-13 0.0 0.0 0.0 2.204855798021098E-5 6.614567394063294E-5 14-15 0.0 0.0 0.0 2.204855798021098E-5 9.921851091094941E-5 16-17 1.102427899010549E-5 0.0 0.0 2.204855798021098E-5 1.1024278990105489E-4 18-19 4.409711596042196E-5 0.0 0.0 2.204855798021098E-5 1.3229134788126588E-4 20-21 6.614567394063294E-5 0.0 0.0 4.409711596042196E-5 1.3229134788126588E-4 22-23 6.614567394063294E-5 0.0 0.0 6.614567394063294E-5 1.4331562687137136E-4 24-25 1.1024278990105489E-4 0.0 0.0 1.5433990586147684E-4 1.5433990586147684E-4 26-27 1.1024278990105489E-4 0.0 0.0 2.7560697475263724E-4 1.5433990586147684E-4 28-29 1.4331562687137136E-4 0.0 0.0 8.819423192084391E-4 1.5433990586147684E-4 30-31 1.5433990586147684E-4 0.0 0.0 0.0032962594180415412 1.5433990586147684E-4 32-33 1.5433990586147684E-4 0.0 0.0 0.008202063568638484 1.7638846384168783E-4 34-35 1.5433990586147684E-4 0.0 0.0 0.014066979991374604 1.7638846384168783E-4 36-37 1.874127428317933E-4 0.0 0.0 0.02439672940510345 1.7638846384168783E-4 38-39 2.4253413778232077E-4 0.0 0.0 0.04115363347006379 1.7638846384168783E-4 40-41 2.4253413778232077E-4 0.0 0.0 0.059685446452431117 1.7638846384168783E-4 42-43 2.9765553273284825E-4 0.0 0.0 0.08075284360252272 1.7638846384168783E-4 44-45 3.307283697031647E-4 0.0 0.0 0.10585512686299291 1.7638846384168783E-4 46-47 3.9687404364379764E-4 0.0 0.0 0.13581911715809963 1.7638846384168783E-4 48-49 4.4097115960421956E-4 0.0 0.0 0.16823049738900975 1.7638846384168783E-4 50-51 4.4097115960421956E-4 0.0 0.0 0.20075212040982093 1.7638846384168783E-4 52-53 4.630197175844305E-4 0.0 0.0 0.2354234778337027 1.984370218218988E-4 54-55 4.630197175844305E-4 2.204855798021098E-5 0.0 0.2747801538283793 2.094613008120043E-4 56-57 4.8506827556464154E-4 2.204855798021098E-5 0.0 0.3230664958050413 2.2048557980210978E-4 58-59 4.8506827556464154E-4 2.204855798021098E-5 0.0 0.37576254937774556 2.2048557980210978E-4 60-61 4.8506827556464154E-4 2.204855798021098E-5 0.0 0.43243836766587795 2.2048557980210978E-4 62-63 4.8506827556464154E-4 2.204855798021098E-5 0.0 0.4922671297451804 2.2048557980210978E-4 64-65 5.071168335448524E-4 2.204855798021098E-5 0.0 0.563186316488529 2.3150985879221526E-4 66-67 5.291653915250635E-4 2.204855798021098E-5 0.0 0.6490103284265003 2.4253413778232077E-4 68-69 5.291653915250635E-4 2.204855798021098E-5 0.0 0.7427828455163376 2.4253413778232077E-4 70-71 5.512139495052745E-4 2.204855798021098E-5 0.0 0.8460693153746359 2.535584167724263E-4 72-73 5.732625074854854E-4 2.204855798021098E-5 0.0 0.9560695711379084 2.6458269576253176E-4 74-75 5.732625074854854E-4 2.204855798021098E-5 0.0 1.079442277316179 2.6458269576253176E-4 76-77 5.842867864755909E-4 2.204855798021098E-5 0.0 1.2260872364425621 2.7560697475263724E-4 78-79 5.953110654656964E-4 2.204855798021098E-5 0.0 1.3898969979565396 2.866312537427427E-4 80-81 6.835052973865404E-4 4.409711596042196E-5 0.0 1.570926683253062 2.976555327328482E-4 82-83 7.276024133469623E-4 5.5121394950527445E-5 0.0 1.7645019980403243 3.086798117229537E-4 84-85 7.386266923370677E-4 6.614567394063294E-5 0.0 1.9805006962934608 3.086798117229537E-4 86-87 7.496509713271732E-4 6.614567394063294E-5 0.0 2.223145076865683 3.197040907130592E-4 88-89 7.496509713271732E-4 6.614567394063294E-5 0.0 2.4969109970269727 3.307283697031647E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6015 0.0 41.339287 1 GGTATCA 3450 0.0 38.81986 1 ATCAACG 7335 0.0 35.9335 3 TCAACGC 7375 0.0 35.673817 4 CAACGCA 7700 0.0 33.982704 5 AACGCAG 7975 0.0 32.868984 6 TATCAAC 7780 0.0 31.0964 2 ACGCAGA 9395 0.0 27.902563 7 CGCAGAG 10065 0.0 25.810104 8 GTACATG 12480 0.0 24.501556 1 ACATGGG 13435 0.0 23.400597 3 TACATGG 13105 0.0 22.966227 2 CATGGGG 11170 0.0 21.300333 4 GCAGAGT 12445 0.0 20.529106 9 AGAGTAC 10585 0.0 19.246593 10-11 TATAAGC 2285 0.0 17.87388 3 CAGAGTA 10885 0.0 17.472761 10-11 ATTCGCG 600 0.0 17.01611 80-81 ATGGGGA 7085 0.0 16.555952 5 GAGTACA 9285 0.0 15.56157 1 >>END_MODULE