FastQCFastQC Report
Fri 27 May 2016
SRR940210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4535444
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63650.041.504351
GGTATCA35050.040.396661
ATCAACG73850.034.7355583
TCAACGC74600.034.6410524
CAACGCA77750.033.2357565
TATCAAC81750.031.7870122
AACGCAG82250.031.4170386
ACGCAGA95450.026.7728277
GTACATG122950.025.4281371
CGCAGAG102100.024.9819818
TACATGG131050.023.2727742
ACATGGG129650.022.900163
CATGGGG109600.020.2412514
GCAGAGT125950.019.9119649
AGAGTAC106200.018.7846510-11
CCTATAA21800.018.090011
GTGGTAT20900.017.9596561
TTATAGG20550.017.5684223
GCTTATA19700.017.3653471
TATAAGC21400.017.3145753
Produced by FastQC (version 0.11.5)