FastQCFastQC Report
Fri 27 May 2016
SRR940209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4232685
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34450.041.3682981
GTATCAA57900.037.4949871
TCAACGC66000.032.7455064
ATCAACG66400.032.6201673
CAACGCA67800.031.8761585
AACGCAG71850.030.081166
TATCAAC72500.029.4838662
ACGCAGA84950.025.3305577
GTACATG115800.024.8599171
CGCAGAG90050.023.8956748
TACATGG122150.022.8661882
ACATGGG123800.022.6754673
CATGGGG105850.020.013744
GCAGAGT110900.019.4457179
CAGAGTA97950.017.72359310-11
AGAGTAC95800.017.30329310-11
TATAAGC19700.016.395783
ATGGGGA64350.015.7960865
GAGTACA85500.015.5570591
CCTATAA20550.015.4881141
Produced by FastQC (version 0.11.5)