FastQCFastQC Report
Fri 27 May 2016
SRR940209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4232685
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34150.043.1317831
GTATCAA60300.042.0775451
ATCAACG69450.035.701473
TCAACGC69900.035.4716344
CAACGCA72450.034.2227445
TATCAAC77400.032.2197072
AACGCAG77600.032.0739366
ACGCAGA92300.027.068667
CGCAGAG98650.025.2781318
GTACATG116350.025.0742761
TACATGG120150.023.9580882
ACATGGG121650.022.841873
CATGGGG102050.020.759164
GCAGAGT119200.020.6811089
AGAGTAC101550.018.70930710-11
GCTTATA20650.018.4075641
CAGAGTA107100.017.71760210-11
GTGGTAT20850.017.0915571
ATGGGGA64400.016.078795
GAGTACT51000.015.92644912-13
Produced by FastQC (version 0.11.5)