FastQCFastQC Report
Fri 27 May 2016
SRR940208_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940208_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4287432
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62500.042.101381
GGTATCA34550.041.5169561
TCAACGC69900.036.4224744
ATCAACG70450.036.205973
CAACGCA74200.034.3117375
TATCAAC76100.034.0172122
AACGCAG76050.033.600416
ACGCAGA89850.028.4932757
CGCAGAG98300.025.754648
GTACATG120550.025.6101881
TACATGG125600.024.0143592
ACATGGG125050.023.2463423
GCAGAGT118200.021.221919
GCTTATA20900.020.680531
CATGGGG104900.020.3759984
AGAGTAC101750.018.74798410-11
CAGAGTA103850.018.55187610-11
TTATAGG21350.018.4657573
CCTATAA20950.017.6838651
TATAAGC20200.017.400723
Produced by FastQC (version 0.11.5)