FastQCFastQC Report
Fri 27 May 2016
SRR940208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4287432
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60700.039.9352951
GGTATCA35000.039.1110841
TCAACGC70700.033.5237464
ATCAACG72500.032.625923
CAACGCA74900.031.771115
AACGCAG77200.031.1321956
TATCAAC77850.030.8718992
ACGCAGA90550.026.1751147
GTACATG115900.026.0414831
CGCAGAG95200.024.846718
TACATGG123150.024.1826882
ACATGGG122600.023.826273
CATGGGG100200.021.6156644
GCAGAGT117800.020.0395349
CCTATAA19600.018.915331
AGAGTAC102000.018.58014910-11
TATAAGC20100.017.0140553
CAGAGTA107900.016.94789510-11
GAGTACA86850.016.8013421
ATGGGGA65050.016.5750585
Produced by FastQC (version 0.11.5)