FastQCFastQC Report
Fri 27 May 2016
SRR940207_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940207_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4459645
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33600.039.8790361
GTATCAA57650.039.7267041
TCAACGC65050.036.081964
ATCAACG66000.035.5637933
CAACGCA66950.034.9842575
AACGCAG70400.033.593686
TATCAAC69100.032.1819532
ACGCAGA85550.027.5348117
CGCAGAG89900.026.3600778
GTACATG120550.023.0580981
ACATGGG128050.021.4473343
TACATGG128300.021.0731852
GCAGAGT112950.020.6870849
TTATAGG22300.019.8154143
TATAGGA23050.019.7884034
GCTTATA21900.019.309931
CATGGGG113850.019.0301514
AGAGTAC96550.017.85751710-11
CAGAGTA102450.017.6404410-11
GAGTACA91350.017.1128431
Produced by FastQC (version 0.11.5)