##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940207_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4459645 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05013695036264 33.0 31.0 34.0 30.0 34.0 2 31.017114590959594 34.0 31.0 34.0 27.0 34.0 3 31.877038418977296 34.0 31.0 34.0 28.0 34.0 4 35.620730125379936 37.0 35.0 37.0 33.0 37.0 5 35.78144269330855 37.0 35.0 37.0 35.0 37.0 6 35.93446855074787 37.0 37.0 37.0 35.0 37.0 7 35.91185217657459 37.0 37.0 37.0 35.0 37.0 8 35.9080626372727 37.0 37.0 37.0 35.0 37.0 9 37.687774475322584 39.0 39.0 39.0 35.0 39.0 10-11 37.63111021168726 39.0 38.0 39.0 35.0 39.0 12-13 37.560579373470304 39.0 38.0 39.0 35.0 39.0 14-15 39.02207204833569 41.0 39.0 41.0 36.0 41.0 16-17 38.94213037136364 41.0 39.0 41.0 36.0 41.0 18-19 38.86671685750772 41.0 39.0 41.0 36.0 41.0 20-21 38.7917050572411 41.0 39.0 41.0 35.5 41.0 22-23 38.693894244945504 40.5 39.0 41.0 35.0 41.0 24-25 38.59018845670451 40.0 39.0 41.0 35.0 41.0 26-27 38.40545110653426 40.0 38.0 41.0 34.0 41.0 28-29 38.24248051134115 40.0 38.0 41.0 34.0 41.0 30-31 38.23306944835295 40.0 38.0 41.0 34.0 41.0 32-33 38.270833552894906 40.0 38.0 41.0 34.0 41.0 34-35 38.286079385242545 40.0 38.0 41.0 34.5 41.0 36-37 38.39911304599357 40.5 39.0 41.0 35.0 41.0 38-39 38.32717346335863 40.5 38.0 41.0 34.0 41.0 40-41 38.20995908867185 40.0 38.0 41.0 34.0 41.0 42-43 37.94559813617452 40.0 38.0 41.0 33.0 41.0 44-45 37.90063996125252 40.0 38.0 41.0 33.0 41.0 46-47 37.749291815828386 40.0 38.0 41.0 33.0 41.0 48-49 37.57317353735555 40.0 38.0 41.0 33.0 41.0 50-51 36.630897975063036 39.0 36.0 40.0 31.5 40.5 52-53 36.699653896218194 39.0 36.5 40.5 31.5 41.0 54-55 37.042891530603896 40.0 36.5 41.0 32.0 41.0 56-57 36.85240383483439 39.5 36.0 41.0 31.5 41.0 58-59 36.571189971399065 39.0 35.5 41.0 31.0 41.0 60-61 36.28276409893613 39.0 35.0 41.0 31.0 41.0 62-63 35.92487821340039 38.0 35.0 40.5 31.0 41.0 64-65 35.52868524288368 37.0 35.0 40.0 30.0 41.0 66-67 35.14132279587277 37.0 35.0 39.5 30.0 41.0 68-69 34.74429377226214 36.0 35.0 39.0 29.0 41.0 70-71 34.324209326078645 36.0 34.0 39.0 29.0 40.5 72-73 33.88909229322065 35.0 34.0 37.5 29.0 40.0 74-75 33.46037162150799 35.0 34.0 37.0 28.5 39.0 76-77 33.0368073019265 35.0 34.0 36.5 27.5 39.0 78-79 32.63438087560782 35.0 33.0 36.0 26.5 37.5 80-81 32.27932514807793 35.0 33.0 35.5 26.0 37.0 82-83 31.96014402491678 35.0 33.0 35.0 25.5 37.0 84-85 31.686619562767888 35.0 33.0 35.0 25.0 36.0 86-87 31.410002141426055 35.0 32.5 35.0 24.5 36.0 88-89 31.12703410249022 35.0 32.0 35.0 23.5 35.5 90-91 30.871125728617415 34.5 32.0 35.0 22.0 35.0 92-93 30.595704702952816 34.0 31.5 35.0 19.5 35.0 94-95 30.284132369280513 34.0 31.0 35.0 18.0 35.0 96-97 29.921074659530074 34.0 31.0 35.0 6.5 35.0 98-99 29.44000127812864 34.0 31.0 35.0 2.0 35.0 100-101 27.393535023527658 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6302151089246806 1101 2 1.6947485490050944 1101 3 0.9602491395450734 1101 4 0.5390747838825973 1101 5 0.44590542647394216 1101 6 0.38469017162601205 1101 7 0.3316181238068978 1101 8 0.39703273845898934 1101 9 0.40479999555732604 1101 10-11 0.41747535758916143 1101 12-13 0.46137536479101726 1101 14-15 0.499426377357274 1101 16-17 0.5531026644002637 1101 18-19 0.600862802296902 1101 20-21 0.5979828587925482 1101 22-23 0.5515686347831021 1101 24-25 0.5330244731308014 1101 26-27 0.6017641996916581 1101 28-29 0.657149867441774 1101 30-31 0.6284800463075939 1101 32-33 0.6219149647129498 1101 34-35 0.5854675871232757 1101 36-37 0.6400683039911215 1101 38-39 0.6804807692904973 1101 40-41 0.6663534301211271 1101 42-43 0.7730549360929189 1101 44-45 0.6550571791076791 1101 46-47 0.6389950535235656 1101 48-49 0.6187245182258039 1101 50-51 0.5006630344562026 1101 52-53 0.6337349507660903 1101 54-55 0.7717909735471622 1101 56-57 0.7995094672482495 1101 58-59 0.802268312087115 1101 60-61 0.7816049697240643 1101 62-63 0.7783708213283589 1101 64-65 0.8461548890894193 1101 66-67 0.7996290070116956 1101 68-69 0.6712534187542616 1101 70-71 0.6387164332880175 1101 72-73 0.7125993414820755 1101 74-75 0.6429226038019067 1101 76-77 0.7410289178736136 1101 78-79 0.7437187144257393 1101 80-81 0.7227274733280993 1101 82-83 0.7376355841176299 1101 84-85 0.7127409030454857 1101 86-87 0.6785210298356077 1101 88-89 0.7651548826226566 1101 90-91 0.7288882648763142 1101 92-93 0.6415259310378509 1101 94-95 0.7385431950542056 1101 96-97 0.685865242804244 1101 98-99 0.7100666924913632 1101 100-101 0.6931293731040924 1102 1 0.3723278833766841 1102 2 0.9034631171083092 1102 3 0.528668375210362 1102 4 0.32812835430171106 1102 5 0.29586683821474935 1102 6 0.20163545066311173 1102 7 0.2068194311825664 1102 8 0.2735227999795242 1102 9 0.2407561515560701 1102 10-11 0.3019435001320474 1102 12-13 0.2714978828918291 1102 14-15 0.3348474065528606 1102 16-17 0.3087167346499342 1102 18-19 0.32062138157181863 1102 20-21 0.3267348754938695 1102 22-23 0.247901858382491 1102 24-25 0.31175099104901705 1102 26-27 0.3829345500029149 1102 28-29 0.40123059776803416 1102 30-31 0.30707787623569516 1102 32-33 0.3565151054021456 1102 34-35 0.3026616871250596 1102 36-37 0.4456796082198622 1102 38-39 0.3149810611586119 1102 40-41 0.33021371915972964 1102 42-43 0.5251543459782297 1102 44-45 0.35295754249934674 1102 46-47 0.3308013437876838 1102 48-49 0.3051374304347192 1102 50-51 0.26015428007553254 1102 52-53 0.28416341194248673 1102 54-55 0.3951945638120975 1102 56-57 0.44014240538823657 1102 58-59 0.43607440827631905 1102 60-61 0.45557698484739007 1102 62-63 0.4948325152637594 1102 64-65 0.498931566000941 1102 66-67 0.4374844066926471 1102 68-69 0.40088230943476333 1102 70-71 0.3455079971357904 1102 72-73 0.3385605488698644 1102 74-75 0.29183241368406954 1102 76-77 0.3547767069462324 1102 78-79 0.403373623840821 1102 80-81 0.39298386079593683 1102 82-83 0.36922091314261607 1102 84-85 0.3228992131548196 1102 86-87 0.3134725609868987 1102 88-89 0.3376458522444885 1102 90-91 0.3600144646567891 1102 92-93 0.23684449351712544 1102 94-95 0.21559812943905143 1102 96-97 0.19924979135788945 1102 98-99 0.3101947758867425 1102 100-101 0.3305995552473284 1103 1 0.29364530500126307 1103 2 1.3693385621208982 1103 3 0.6819275498905206 1103 4 0.21577871815259186 1103 5 0.19659589345324946 1103 6 0.14411786484825484 1103 7 0.1680307031858348 1103 8 0.14300599731215868 1103 9 0.17532629086111484 1103 10-11 0.13963154537164968 1103 12-13 0.23263541395169796 1103 14-15 0.2325944165442877 1103 16-17 0.28159672336477115 1103 18-19 0.1879287835290384 1103 20-21 0.21807333662305695 1103 22-23 0.2874782843363164 1103 24-25 0.25857189880003517 1103 26-27 0.25519344628767726 1103 28-29 0.24967067345050964 1103 30-31 0.21594707987083694 1103 32-33 0.24992750139474396 1103 34-35 0.2710330647178978 1103 36-37 0.28235371548773713 1103 38-39 0.30199652948827094 1103 40-41 0.41848754985239367 1103 42-43 0.5353086314004827 1103 44-45 0.3972693154743183 1103 46-47 0.27258555258470096 1103 48-49 0.37021075016797766 1103 50-51 0.3425386654774414 1103 52-53 0.37698774141328784 1103 54-55 0.4429600848805393 1103 56-57 0.5401494761179251 1103 58-59 0.46236801344304723 1103 60-61 0.5332836041705065 1103 62-63 0.423002069028378 1103 64-65 0.46205998585387675 1103 66-67 0.49221194207429875 1103 68-69 0.47266640505343105 1103 70-71 0.426344347780514 1103 72-73 0.5711727086114138 1103 74-75 0.4985033379567909 1103 76-77 0.43654669144196845 1103 78-79 0.5283780868013963 1103 80-81 0.46761882562430657 1103 82-83 0.49163510878760874 1103 84-85 0.4630948646793911 1103 86-87 0.41234341445897726 1103 88-89 0.47028635324419454 1103 90-91 0.4047087104362852 1103 92-93 0.525178057874296 1103 94-95 0.49814270887298306 1103 96-97 0.41552197314705097 1103 98-99 0.44102766117384107 1103 100-101 0.3749068360920411 1104 1 0.46093593731256277 1104 2 1.4344550367123752 1104 3 0.7343443931434201 1104 4 0.4058650209069796 1104 5 0.2727007929075782 1104 6 0.1413522261183786 1104 7 0.195316779210728 1104 8 0.15865105536864377 1104 9 0.11412978202517365 1104 10-11 0.16553192045165588 1104 12-13 0.20908273550618617 1104 14-15 0.18111792888061728 1104 16-17 0.12837639391995026 1104 18-19 0.12203399984415597 1104 20-21 0.1592229988792866 1104 22-23 0.0756000049853327 1104 24-25 0.14948776886992476 1104 26-27 0.2280770444702256 1104 28-29 0.15519937443879428 1104 30-31 0.06103955711652986 1104 32-33 0.15382065150630098 1104 34-35 0.05124953804373433 1104 36-37 0.17808668320319043 1104 38-39 0.18494596103730032 1104 40-41 0.26267990062496693 1104 42-43 0.37314862393323267 1104 44-45 0.19124199945246545 1104 46-47 0.16629457764789635 1104 48-49 0.09545035071545982 1104 50-51 0.022351740211384197 1104 52-53 0.0722795335887696 1104 54-55 0.2421934038097362 1104 56-57 0.17492402130291396 1104 58-59 0.10807482093271403 1104 60-61 0.09067202035472377 1104 62-63 0.0825421553085306 1104 64-65 0.0934072589526167 1104 66-67 0.06094559052063886 1104 68-69 0.018210906140069483 1104 70-71 -0.0228694442069326 1104 72-73 0.019346476244422206 1104 74-75 -0.00915460200509699 1104 76-77 -0.01651649584309922 1104 78-79 -6.198172164531002E-4 1104 80-81 -0.04795385392411333 1104 82-83 -0.00715750921478886 1104 84-85 -0.05506406112184692 1104 86-87 -0.227649163495105 1104 88-89 -0.2332487351332979 1104 90-91 -0.13525959444436708 1104 92-93 -0.24898483750106948 1104 94-95 -0.4285203469668417 1104 96-97 -0.5031952672440703 1104 98-99 -0.39378864996989904 1104 100-101 -0.32289622475607516 1105 1 0.2972736070418449 1105 2 1.3322364549104542 1105 3 0.6314265735425941 1105 4 0.3830423126014111 1105 5 0.22519792624152757 1105 6 0.13428749422587316 1105 7 0.09866680892201174 1105 8 0.12176024114751982 1105 9 0.07621523704325739 1105 10-11 0.055151202568978874 1105 12-13 -0.02514059482302855 1105 14-15 0.07376810281587609 1105 16-17 -0.006146721282860312 1105 18-19 -0.006485500742378747 1105 20-21 0.04763794037818769 1105 22-23 -0.0695768926112379 1105 24-25 -0.04429231352995089 1105 26-27 0.006305238125335677 1105 28-29 -0.0070339662398879454 1105 30-31 -0.01216549428553293 1105 32-33 -0.08976239296121236 1105 34-35 -0.022114735929932294 1105 36-37 0.05138042861509717 1105 38-39 0.014363056879169278 1105 40-41 -0.08781164408468811 1105 42-43 0.07267176992493773 1105 44-45 -0.014308292668175682 1105 46-47 -0.042016873577878755 1105 48-49 -0.17939815692480465 1105 50-51 -0.2269304386923281 1105 52-53 -0.12984802304410437 1105 54-55 7.178723440404156E-5 1105 56-57 -0.0638887237162109 1105 58-59 -0.03960350944708324 1105 60-61 -0.13718922465142924 1105 62-63 -0.1009450345318399 1105 64-65 -0.02393823687395269 1105 66-67 -0.10226777168387713 1105 68-69 -0.18827053013549744 1105 70-71 -0.19359029361096702 1105 72-73 -0.34265428732017256 1105 74-75 -0.35057969680190126 1105 76-77 -0.28821810350725485 1105 78-79 -0.2380857130171563 1105 80-81 -0.22177107652826322 1105 82-83 -0.1888870380083425 1105 84-85 -0.2004332243720448 1105 86-87 -0.2747011927691858 1105 88-89 -0.27989715498122436 1105 90-91 -0.374133422432088 1105 92-93 -0.5185686640065263 1105 94-95 -0.6809975334952014 1105 96-97 -0.880869282780143 1105 98-99 -0.9614248149579083 1105 100-101 -0.8840953664496531 1106 1 0.22687241893672194 1106 2 1.2483307619464732 1106 3 0.4799484148671489 1106 4 0.2481306397784806 1106 5 0.0420086363512695 1106 6 0.10496449244872252 1106 7 0.11686306558036819 1106 8 0.10815901204037459 1106 9 -0.002060246488589712 1106 10-11 0.07599131494313127 1106 12-13 0.04931067534572975 1106 14-15 0.032338687678738154 1106 16-17 -0.002941739775486951 1106 18-19 -0.022377509281284347 1106 20-21 -0.15148644593237748 1106 22-23 -0.10250991985970614 1106 24-25 -0.02026593278088029 1106 26-27 0.0251051124970445 1106 28-29 -0.06071637100852456 1106 30-31 -0.07075544783644006 1106 32-33 -0.02568099917850475 1106 34-35 -0.08005348439087356 1106 36-37 -0.023286191566718628 1106 38-39 0.022768352338083275 1106 40-41 0.04280512645774337 1106 42-43 0.12770441063398863 1106 44-45 0.03398011126849809 1106 46-47 0.003247660983610956 1106 48-49 -0.12621281228731362 1106 50-51 -0.3231201111886719 1106 52-53 -0.19928113432287375 1106 54-55 -0.05554939340929366 1106 56-57 -0.026597503274324197 1106 58-59 -0.07690644049574757 1106 60-61 -0.07493450673700153 1106 62-63 -0.1212661256624088 1106 64-65 -0.1796799503532469 1106 66-67 0.013401854518320988 1106 68-69 0.07878708604981455 1106 70-71 -0.03515803097871384 1106 72-73 -0.03200508465456409 1106 74-75 -0.09666096424118109 1106 76-77 -0.13102853589980867 1106 78-79 -0.09596117056516817 1106 80-81 -0.1469758482578456 1106 82-83 -0.07830479140739399 1106 84-85 -0.045338216487500915 1106 86-87 -0.13465741357461525 1106 88-89 -0.33860481055947034 1106 90-91 -0.5224961127368992 1106 92-93 -0.593254325098588 1106 94-95 -0.553005667152263 1106 96-97 -0.6686476108273176 1106 98-99 -0.6677694607969791 1106 100-101 -0.5870102944040916 1107 1 0.4270633768557275 1107 2 1.4478249469904476 1107 3 0.7713282721064125 1107 4 0.3893693896888877 1107 5 0.32262552055100713 1107 6 0.2282004734338301 1107 7 0.23092142873535693 1107 8 0.22239910809412322 1107 9 0.08869770246298714 1107 10-11 0.20167600658972873 1107 12-13 0.315722021836784 1107 14-15 0.2821942096694201 1107 16-17 0.2773166104644744 1107 18-19 0.2992450156635229 1107 20-21 0.21392331172634016 1107 22-23 0.1970853855984558 1107 24-25 0.12092250528836956 1107 26-27 0.2786746276370735 1107 28-29 0.17030261763122212 1107 30-31 0.07892135712747717 1107 32-33 0.11122316208620475 1107 34-35 0.17938530795463947 1107 36-37 0.22401250962139585 1107 38-39 0.25616665110242565 1107 40-41 0.17200348639106977 1107 42-43 0.3095402748161433 1107 44-45 0.05506705282657265 1107 46-47 -0.009978250537095334 1107 48-49 0.18051151785203956 1107 50-51 0.14831496844099235 1107 52-53 0.20535887345452863 1107 54-55 0.17836266802082434 1107 56-57 0.2257178703638303 1107 58-59 0.3326005815186974 1107 60-61 0.3414216670368333 1107 62-63 0.29587002357286707 1107 64-65 0.23423006738008212 1107 66-67 0.3312201585196348 1107 68-69 0.2493494787646 1107 70-71 0.09734380870320791 1107 72-73 0.1735418135743032 1107 74-75 0.10001859616532727 1107 76-77 0.022607257462574637 1107 78-79 -0.03935424654881814 1107 80-81 -0.022207343044648553 1107 82-83 -0.024515649823914742 1107 84-85 -0.06016062420358992 1107 86-87 0.05837191276473419 1107 88-89 0.10867434068189752 1107 90-91 0.030858601354889714 1107 92-93 -0.07366073691342478 1107 94-95 -0.0545641633604248 1107 96-97 -0.029170449493772566 1107 98-99 0.04216294109315655 1107 100-101 -0.11042137336955449 1108 1 0.19613849220746715 1108 2 1.2967553217973098 1108 3 0.5813144493666762 1108 4 0.17664940134648077 1108 5 0.07432793578422547 1108 6 0.014985686465081471 1108 7 -0.04093056470666312 1108 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-1.7785512681646871 2316 90-91 -1.565214165720434 2316 92-93 -1.8475488519741639 2316 94-95 -1.769507846089681 2316 96-97 -1.4891090257580935 2316 98-99 -1.453700331781473 2316 100-101 -1.7289541771761883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25716.0 3 8374.0 4 6398.0 5 5164.0 6 4640.0 7 4317.0 8 4728.0 9 5266.0 10 6132.0 11 7051.0 12 7757.0 13 8871.0 14 9450.0 15 10621.0 16 12409.0 17 14853.0 18 14331.0 19 12481.0 20 13016.0 21 14163.0 22 15127.0 23 16683.0 24 19158.0 25 22005.0 26 26138.0 27 31325.0 28 38223.0 29 47448.0 30 59599.0 31 75233.0 32 98089.0 33 133319.0 34 195318.0 35 314142.0 36 607967.0 37 1231472.0 38 1192350.0 39 140309.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.959702105022494 17.513524210305256 13.281558678278943 25.245215006393302 2 17.575924961595252 19.793091116235985 37.01930681418159 25.61167710798718 3 19.179616533318136 22.8246882843935 28.496744461972952 29.498950720315413 4 13.855504271697793 15.789205619250898 34.987265792559974 35.36802431649133 5 15.747978318727625 35.630211261822595 33.389881923313204 15.231928496136582 6 34.77474988405815 33.591716952985465 17.124274410356097 14.509258752600282 7 30.813686718973916 30.177024730446345 20.330842661578576 18.678445889001168 8 27.65717625355153 33.53298986250477 19.542156510295715 19.267677373647984 9 27.11695727559845 15.867323919152742 19.024203408013506 37.991515397235304 10-11 26.245431700580816 24.934748991758813 26.878661101002628 21.941158206657736 12-13 26.81406901968154 21.933843757123974 27.00322686303768 24.248860360156804 14-15 24.02979911333789 23.379497999207196 24.220509401791826 28.370193485663087 16-17 24.710565312384446 26.486510917538435 24.730741542049383 24.07218222802773 18-19 24.720076450399258 25.776361049369427 25.838918308944265 23.664644191287046 20-21 25.050257097647982 25.19737511314506 25.774957436373814 23.977410352833143 22-23 25.058678819096546 24.840795401190878 25.604721801674955 24.49580397803762 24-25 24.98733269145576 25.064956747266145 25.463717677620107 24.48399288365799 26-27 24.76763425364656 25.354205547534566 25.430173213005038 24.447986985813838 28-29 24.69701057611892 25.34766493100527 25.53459783857564 24.42072665430016 30-31 24.795235693410962 25.12805268196892 25.4185050282502 24.658206596369915 32-33 24.719045684393464 25.174596663456896 25.42361116328661 24.68274648886303 34-35 25.06072274732037 25.1864913628978 25.22888149815261 24.523904391629227 36-37 24.97898184078143 25.047433138031078 25.23644798323795 24.737137037949537 38-39 24.804595432823305 25.195281226943788 25.539770497464797 24.460352842768113 40-41 24.965083062841067 24.92479515172609 25.540920990842658 24.569200794590184 42-43 25.013203322191334 24.975247274957642 25.27138293446779 24.74016646838323 44-45 24.873203238272723 25.14194161119443 25.224893444965996 24.75996170556686 46-47 24.912639137374907 25.11440199733233 25.158616467226853 24.81434239806591 48-49 25.048060254057763 25.190514166225235 25.085187529182644 24.67623805053436 50-51 24.773393469448443 25.234349305802166 25.205878767987095 24.78637845676229 52-53 25.15086877021222 25.076728090627448 25.02123471142826 24.75116842773207 54-55 24.901530879745682 24.904457370338527 25.190132187673935 25.00387956224186 56-57 24.813523774688527 25.29252387347571 25.29619054870169 24.597761803134077 58-59 24.985305373512627 24.977691751700394 25.248216405370304 24.788786469416678 60-61 25.064952044814838 24.890262721349956 25.3990584112029 24.64572682263231 62-63 24.99565176874609 25.05394217766374 25.30373418586701 24.64667186772316 64-65 24.881611476625114 24.999184223430607 25.201855642196698 24.917348657747585 66-67 24.808046768285923 25.232216004477404 25.089319886019517 24.87041734121716 68-69 24.606676000069978 25.059122519534434 25.435781863920415 24.898419616475177 70-71 24.649406149935157 25.072308807640574 25.269759819145282 25.008525223278987 72-73 24.840862189268858 25.013552591118604 25.21028664655403 24.935298573058503 74-75 24.714753412152284 25.103752287956528 25.17642179133971 25.00507250855148 76-77 24.909831440823694 24.997908013150912 25.21425198290067 24.87800856312472 78-79 24.6932921133187 25.08638067253271 25.269925932505817 24.95040128164277 80-81 24.646541897508673 25.191042143372577 25.365395257770224 24.797020701348522 82-83 24.77648352469034 25.114201385341833 25.212476755755542 24.896838334212287 84-85 24.698236433802094 25.025722388396794 25.16739036991906 25.108650807882043 86-87 24.807186685723803 24.902363955772344 25.443114843538417 24.84733451496544 88-89 24.69360845847891 24.912204839376013 25.318861616704876 25.0753250854402 90-91 24.617126139223842 25.09568201810557 25.428169180880282 24.85902266179031 92-93 24.69509972201539 24.981407493671817 25.45890119465406 24.86459158965873 94-95 24.698338729749132 24.87296066366882 25.34191418986385 25.086786416718198 96-97 24.877689130584816 24.796442192073822 25.276708107468153 25.04916056987321 98-99 24.552724636477592 24.798585525540556 25.33013569034187 25.318554147639983 100-101 24.529935245122626 24.70325075992446 25.600584376020386 25.16622961893253 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 119.0 1 104.0 2 134.5 3 172.5 4 136.0 5 144.5 6 222.0 7 455.5 8 841.5 9 996.5 10 896.0 11 831.0 12 874.5 13 928.5 14 982.0 15 1073.5 16 1121.5 17 1186.5 18 1291.0 19 1390.5 20 1550.0 21 1743.0 22 2030.0 23 2387.5 24 2830.5 25 3744.0 26 5022.0 27 6740.0 28 9135.0 29 12285.0 30 16657.0 31 22035.5 32 27318.0 33 32822.0 34 39425.0 35 46201.0 36 53761.0 37 62631.5 38 72612.0 39 79837.0 40 86174.0 41 97897.0 42 110898.0 43 123955.0 44 136676.5 45 147272.0 46 157536.0 47 169548.5 48 186445.5 49 199745.0 50 206803.0 51 211920.0 52 212854.0 53 215608.0 54 217459.0 55 209676.5 56 199256.5 57 185094.5 58 167241.0 59 148510.0 60 125479.5 61 101178.0 62 80237.0 63 63137.5 64 48057.5 65 35937.0 66 26740.5 67 20447.0 68 14769.0 69 10256.0 70 7607.0 71 5507.5 72 4046.5 73 3072.0 74 2279.5 75 1694.5 76 1174.0 77 729.5 78 430.0 79 295.0 80 222.5 81 167.0 82 137.5 83 115.5 84 96.0 85 80.5 86 67.5 87 65.0 88 60.5 89 53.0 90 45.5 91 42.5 92 39.0 93 33.0 94 29.0 95 30.5 96 29.0 97 23.0 98 20.0 99 17.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05915269040472952 2 0.05637220002937454 3 0.06229195373174322 4 0.061708947685297826 5 0.06078959199667238 6 0.011503157762557333 7 0.013610948853552246 8 0.0146648443990497 9 0.00612156348767671 10-11 0.011626485964690014 12-13 0.015034829005447744 14-15 0.017658356214452046 16-17 0.032031697590279046 18-19 0.02981178995189079 20-21 0.029587556857104097 22-23 0.01880194499786418 24-25 0.007433327092178862 26-27 0.011458311143599995 28-29 0.018521653629380814 30-31 0.016548402395257917 32-33 0.019474644282224257 34-35 0.023779919702128758 36-37 0.022434521133408603 38-39 0.009754139623221131 40-41 0.011009844954026609 42-43 0.012108587118481404 44-45 0.023174490346204685 46-47 0.01564025836137181 48-49 0.014776960946443046 50-51 0.014563939506395689 52-53 0.012433725105922108 54-55 0.008498434292415652 56-57 0.0126579582007088 58-59 0.01192920064265205 60-61 0.01314005935450019 62-63 0.0209770060172951 64-65 0.01577479821824383 66-67 0.01819651564194011 68-69 0.024822603592886877 70-71 0.018331055498812126 72-73 0.024396560712792163 74-75 0.04017135893103599 76-77 0.048434348473925616 78-79 0.036247279772268874 80-81 0.01967645406753228 82-83 0.01671657721634794 84-85 0.021986054943835216 86-87 0.02517016488980625 88-89 0.02177303350378786 90-91 0.025271069782460263 92-93 0.04942097409098706 94-95 0.07728193611823363 96-97 0.09176739404145397 98-99 0.09597176456870446 100-101 0.08094814721799605 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4459645.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.1721197117111 #Duplication Level Percentage of deduplicated Percentage of total 1 78.00249321488492 41.47557907033793 2 11.505355677856501 12.235282988576019 3 3.8581959944729523 6.154453778680802 4 1.9305512686488862 4.1060601266477725 5 1.147907094903126 3.0518326734055767 6 0.6937003202758135 2.213130988425473 7 0.5207414784553874 1.9382249761898298 8 0.3645704321308625 1.5507986128489974 9 0.28174540923469277 1.3482900565246905 >10 1.5307833544195133 14.647235246907497 >50 0.10337836032619153 3.788148422321618 >100 0.056442116024891305 5.489701416861805 >500 0.00306948520188115 1.1321177981342203 >1k 0.001065793164414308 0.8691438441377684 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2423309478669266E-5 2 0.0 0.0 0.0 0.0 2.2423309478669266E-5 3 0.0 0.0 0.0 2.2423309478669266E-5 2.2423309478669266E-5 4 0.0 0.0 0.0 2.2423309478669266E-5 2.2423309478669266E-5 5 0.0 0.0 0.0 2.2423309478669266E-5 2.2423309478669266E-5 6 2.2423309478669266E-5 0.0 0.0 2.2423309478669266E-5 2.2423309478669266E-5 7 2.2423309478669266E-5 0.0 0.0 2.2423309478669266E-5 2.2423309478669266E-5 8 2.2423309478669266E-5 0.0 0.0 2.2423309478669266E-5 2.2423309478669266E-5 9 2.2423309478669266E-5 0.0 0.0 2.2423309478669266E-5 2.2423309478669266E-5 10-11 2.2423309478669266E-5 0.0 0.0 2.2423309478669266E-5 5.6058273696673165E-5 12-13 6.72699284360078E-5 0.0 0.0 2.2423309478669266E-5 7.848158317534243E-5 14-15 8.969323791467706E-5 0.0 0.0 3.36349642180039E-5 1.009048926540117E-4 16-17 1.009048926540117E-4 0.0 0.0 4.484661895733853E-5 1.345398568720156E-4 18-19 1.2332820213268095E-4 0.0 0.0 4.484661895733853E-5 1.345398568720156E-4 20-21 1.345398568720156E-4 0.0 0.0 4.484661895733853E-5 1.6817482109001948E-4 22-23 1.345398568720156E-4 0.0 0.0 4.484661895733853E-5 2.354447495260273E-4 24-25 2.1302144004735801E-4 0.0 0.0 7.848158317534243E-5 2.4665640426536195E-4 26-27 2.2423309478669266E-4 0.0 0.0 2.1302144004735804E-4 2.4665640426536195E-4 28-29 2.578680590046966E-4 0.0 0.0 8.184507959714282E-4 2.4665640426536195E-4 30-31 3.139263327013697E-4 0.0 0.0 0.003184109945971036 2.690797137440312E-4 32-33 3.139263327013697E-4 0.0 0.0 0.007455750401657532 3.139263327013697E-4 34-35 3.699846063980429E-4 1.1211654739334633E-5 0.0 0.01357731388933424 3.5877295165870826E-4 36-37 4.260428800947161E-4 3.36349642180039E-5 0.0 0.023409935095730715 3.5877295165870826E-4 38-39 4.708894990520546E-4 4.484661895733853E-5 0.0 0.03950987130141525 3.5877295165870826E-4 40-41 4.708894990520546E-4 5.6058273696673165E-5 0.0 0.05721307413482463 3.699846063980429E-4 42-43 4.8210115379138926E-4 8.969323791467706E-5 0.0 0.07671014172652757 3.8119626113737755E-4 44-45 5.157361180093931E-4 8.969323791467706E-5 0.0 0.09909981624097883 4.1483122535538144E-4 46-47 5.381594274880624E-4 8.969323791467706E-5 0.0 0.12441573264239641 4.708894990520546E-4 48-49 5.605827369667317E-4 8.969323791467706E-5 0.0 0.15217578977698898 5.381594274880624E-4 50-51 5.605827369667317E-4 8.969323791467706E-5 0.0 0.1830302636196379 5.381594274880624E-4 52-53 5.605827369667317E-4 8.969323791467706E-5 0.0 0.21540952250683632 6.054293559240702E-4 54-55 5.605827369667317E-4 8.969323791467706E-5 0.0 0.25167922558858385 6.054293559240702E-4 56-57 5.605827369667317E-4 8.969323791467706E-5 0.0 0.2954943723099036 6.054293559240702E-4 58-59 5.605827369667317E-4 8.969323791467706E-5 0.0 0.3415966965980476 6.054293559240702E-4 60-61 5.605827369667317E-4 8.969323791467706E-5 0.0 0.39097282407007733 6.054293559240702E-4 62-63 5.717943917060663E-4 8.969323791467706E-5 0.0 0.4428715738584573 6.72699284360078E-4 64-65 5.83006046445401E-4 8.969323791467706E-5 0.0 0.5035154143435183 6.951225938387473E-4 66-67 6.502759748814087E-4 8.969323791467706E-5 0.0 0.5742721674034592 7.175459033174165E-4 68-69 7.399692127960858E-4 8.969323791467706E-5 0.0 0.6553436428235879 7.399692127960858E-4 70-71 7.511808675354204E-4 8.969323791467706E-5 0.0 0.7452499021783123 8.296624507107629E-4 72-73 7.848158317534243E-4 8.969323791467706E-5 0.0 0.8433518811474905 0.001009048926540117 74-75 8.072391412320936E-4 8.969323791467706E-5 0.0 0.9535960821993679 0.0010314722360187862 76-77 8.072391412320936E-4 8.969323791467706E-5 0.0 1.081218348097214 0.0010314722360187862 78-79 8.072391412320936E-4 8.969323791467706E-5 0.0 1.2240099828573798 0.0010314722360187862 80-81 9.417789981041092E-4 8.969323791467706E-5 0.0 1.3831257869180171 0.0010426838907581209 82-83 9.866256170614478E-4 8.969323791467706E-5 0.0 1.553800807014908 0.0010875305097154593 84-85 9.866256170614478E-4 8.969323791467706E-5 0.0 1.7464394587461558 0.001098742164454794 86-87 9.866256170614478E-4 8.969323791467706E-5 0.0 1.9597187668525184 0.0011211654739334631 88-89 9.866256170614478E-4 8.969323791467706E-5 0.0 2.20317984951717 0.0011435887834121325 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3360 0.0 39.879036 1 GTATCAA 5765 0.0 39.726704 1 TCAACGC 6505 0.0 36.08196 4 ATCAACG 6600 0.0 35.563793 3 CAACGCA 6695 0.0 34.984257 5 AACGCAG 7040 0.0 33.59368 6 TATCAAC 6910 0.0 32.181953 2 ACGCAGA 8555 0.0 27.534811 7 CGCAGAG 8990 0.0 26.360077 8 GTACATG 12055 0.0 23.058098 1 ACATGGG 12805 0.0 21.447334 3 TACATGG 12830 0.0 21.073185 2 GCAGAGT 11295 0.0 20.687084 9 TTATAGG 2230 0.0 19.815414 3 TATAGGA 2305 0.0 19.788403 4 GCTTATA 2190 0.0 19.30993 1 CATGGGG 11385 0.0 19.030151 4 AGAGTAC 9655 0.0 17.857517 10-11 CAGAGTA 10245 0.0 17.64044 10-11 GAGTACA 9135 0.0 17.112843 1 >>END_MODULE