FastQCFastQC Report
Fri 27 May 2016
SRR940207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940207_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4459645
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33950.042.8283541
GTATCAA60900.040.104731
TCAACGC69550.034.0149574
ATCAACG71050.033.2972153
CAACGCA73350.032.2516825
TATCAAC76400.031.1531222
AACGCAG78500.030.3747986
GTACATG120850.025.6360241
ACGCAGA92700.025.5679117
CGCAGAG98150.024.0514098
TACATGG129150.023.8362432
ACATGGG127250.023.2580263
CATGGGG111500.019.8966394
AGAGTAC102300.018.7345110-11
GCAGAGT124650.018.671469
ATGGGGA69600.016.994695
GTATTAG7050.016.8500291
TTATAGG20250.016.65623
CAGAGTA109600.016.61993410-11
GCTTATA20500.016.4571381
Produced by FastQC (version 0.11.5)