FastQCFastQC Report
Fri 27 May 2016
SRR940206_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940206_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4548411
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55950.040.0598831
GGTATCA31350.039.191811
TCAACGC69750.033.8391764
ATCAACG69850.033.5187723
CAACGCA74250.031.5310885
TATCAAC71500.030.5420512
AACGCAG77650.030.2112836
ACGCAGA88400.026.4314147
CGCAGAG95500.024.317448
GTACATG120500.023.8180941
ACATGGG127600.022.5170923
TACATGG128650.021.5630872
CATGGGG111650.019.821424
TTATAGG21850.019.561343
GCAGAGT117950.019.4058559
AGAGTAC100350.018.71588710-11
TATAGGA25500.017.5063344
GCTTATA22600.016.7768921
CAGAGTA106700.016.33364110-11
TAGCGCG2457.351409E-515.50713354
Produced by FastQC (version 0.11.5)