FastQCFastQC Report
Fri 27 May 2016
SRR940206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4548411
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34500.044.2066081
GTATCAA58800.039.5931471
TCAACGC69450.032.489744
ATCAACG70600.031.893233
CAACGCA73850.030.551985
TATCAAC76600.029.705472
AACGCAG76700.029.4167376
GTACATG123150.025.5016521
ACGCAGA90350.024.867067
TACATGG129400.023.8621042
CGCAGAG95950.023.6132268
ACATGGG128550.023.465323
CATGGGG109900.020.2289354
GCAGAGT120800.018.6770639
AGAGTAC103000.017.89272110-11
ATTCGCG6200.017.62068680-81
TATAAGC22650.017.4074343
CCTATAA22350.016.7938731
CAGAGTA111100.016.35306710-11
CTATAAG25200.016.2116622
Produced by FastQC (version 0.11.5)