FastQCFastQC Report
Fri 27 May 2016
SRR940205_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940205_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4254190
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53300.043.2272341
GGTATCA29500.039.6146661
ATCAACG63150.036.4804533
TCAACGC63850.036.0061154
CAACGCA66200.034.871465
TATCAAC67400.033.901022
AACGCAG69800.033.0740976
ACGCAGA81550.028.2504237
GTACATG118000.027.2552131
CGCAGAG87400.026.1961618
TACATGG125150.025.2037282
ACATGGG123400.025.0201573
CATGGGG110150.020.9576994
GCAGAGT111350.020.6877239
GCTTATA19350.019.3949661
TTATAGG20350.019.1399733
AGAGTAC94550.018.43617410-11
ATGGGGA65000.018.2691575
CAGAGTA98800.017.16238210-11
TATAGGA23100.016.8614034
Produced by FastQC (version 0.11.5)