FastQCFastQC Report
Fri 27 May 2016
SRR940205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940205_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4254190
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31100.044.459111
GTATCAA57750.038.9990961
TCAACGC64350.033.215754
ATCAACG65600.032.7276423
CAACGCA69350.030.8894525
TATCAAC71200.030.7546842
AACGCAG72000.029.7525486
ACGCAGA86450.024.779457
GTACATG115950.023.6446691
CGCAGAG93000.022.9831568
TACATGG122100.021.8630642
ACATGGG121850.021.1277793
CATGGGG106150.018.4803494
GCAGAGT116350.018.2890769
GAGTACA89350.017.2829151
AGAGTAC99250.017.18072710-11
AGTACAT87200.017.15872
CAGAGTA103600.016.41348610-11
GTATAGG10150.016.3843861
ATGGGGA64200.016.2027685
Produced by FastQC (version 0.11.5)