FastQCFastQC Report
Fri 27 May 2016
SRR940204_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940204_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4302789
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53250.041.5655941
GGTATCA30350.039.1245161
TCAACGC61150.035.4954154
ATCAACG62650.034.343123
CAACGCA65200.033.145645
TATCAAC67600.032.3220562
AACGCAG67900.031.9667846
ACGCAGA82200.026.4062677
GTACATG119350.026.265711
CGCAGAG86900.024.8141128
TACATGG128050.024.0742632
ACATGGG125950.023.8708653
CATGGGG110950.020.80474
TATAAGC21200.019.4915353
GCAGAGT111350.019.1979839
CCTATAA21000.018.546511
GAGTACA88550.018.0762771
AGAGTAC94500.017.99802810-11
GTATAGG8650.017.5712031
AGTACAT86050.017.2223172
Produced by FastQC (version 0.11.5)