FastQCFastQC Report
Fri 27 May 2016
SRR940204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940204_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4302789
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33350.042.7553331
GTATCAA57400.038.7524221
TCAACGC64300.033.166714
ATCAACG65450.032.511383
CAACGCA68050.031.2695755
AACGCAG70900.030.2135946
TATCAAC73500.029.27442
ACGCAGA82750.025.7147397
GTACATG111750.025.3066311
CGCAGAG89100.023.9887128
TACATGG119850.023.4617732
ACATGGG118700.023.0482943
CATGGGG101700.020.6427884
CCTATAA20950.019.9647351
TATAAGC22200.019.0416283
GCAGAGT115350.018.4472899
AGAGTAC96000.018.35596810-11
GAGTACA86650.017.4979421
GTATAAC6900.017.22091
AGTACAT81800.017.187612
Produced by FastQC (version 0.11.5)