FastQCFastQC Report
Fri 27 May 2016
SRR940203_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940203_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16948543
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147300.044.8696861
GTATCAA253500.043.597381
ATCAACG310200.036.2710043
TCAACGC315350.035.7225954
CAACGCA322700.034.8337975
TATCAAC323600.033.4192122
AACGCAG339300.033.2298286
ACGCAGA404100.027.7019067
CGCAGAG417050.026.6815038
GTACATG552700.024.4924161
TACATGG571850.023.3649942
ACATGGG596350.023.0816563
GCAGAGT506450.021.5307989
CATGGGG466800.021.2013114
AGAGTAC449950.018.8109710-11
CAGAGTA462800.018.38616610-11
ATGGGGA290100.017.24515
CGTATCA42700.016.53153494-95
TCGCCGT111700.016.51175194-95
TAGGACG96100.015.7714494
Produced by FastQC (version 0.11.5)