FastQCFastQC Report
Fri 27 May 2016
SRR940203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940203_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16948543
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167550.046.961191
GTATCAA280100.043.7483251
ATCAACG343050.035.0470543
TCAACGC346300.034.8003434
CAACGCA355100.033.9105765
TATCAAC364700.033.3187942
AACGCAG368850.032.7214056
ACGCAGA436800.027.5114677
CGCAGAG452650.026.4221238
GTACATG583650.024.4307441
TACATGG599250.023.3692052
ACATGGG618050.022.0968843
GCAGAGT545950.021.663129
CATGGGG472650.020.803974
AGAGTAC490000.018.82468210-11
CAGAGTA501550.018.81260310-11
GAGTACT173700.016.99479312-13
ATGGGGA295000.016.8268225
GTACTTT188800.015.8122114-15
GTATATA62150.015.59596351
Produced by FastQC (version 0.11.5)