FastQCFastQC Report
Fri 27 May 2016
SRR940202_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940202_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17280129
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156500.043.9817161
GTATCAA259400.042.596381
ATCAACG318500.036.6797873
TCAACGC326500.035.7805334
CAACGCA337150.034.620415
AACGCAG352950.033.3268176
TATCAAC332750.032.802232
ACGCAGA417400.027.9655287
CGCAGAG428900.027.0503168
GTACATG574350.024.0185891
ACATGGG614450.023.163353
TACATGG589600.023.0632292
GCAGAGT517350.021.8728589
CATGGGG482450.021.448644
CAGAGTA477550.018.63988510-11
AGAGTAC467450.018.57532510-11
ATGGGGA298100.018.0325835
TAGGACG96400.017.0448784
TCGCCGT113050.016.6450494-95
CGTATCA42500.016.60342894-95
Produced by FastQC (version 0.11.5)