##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940202_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17280129 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42605874064945 33.0 31.0 34.0 28.0 34.0 2 29.63304423248229 33.0 31.0 34.0 16.0 34.0 3 30.983367658887268 33.0 31.0 34.0 25.0 34.0 4 35.075872813218005 37.0 35.0 37.0 32.0 37.0 5 35.39089071615148 37.0 35.0 37.0 33.0 37.0 6 35.622731751597456 37.0 35.0 37.0 35.0 37.0 7 35.68021668125279 37.0 36.0 37.0 35.0 37.0 8 35.71158675956644 37.0 36.0 37.0 35.0 37.0 9 37.476111839211384 39.0 38.0 39.0 35.0 39.0 10-11 37.404488820656375 39.0 38.0 39.0 35.0 39.0 12-13 37.32929690513306 39.0 38.0 39.0 35.0 39.0 14-15 38.72085489060875 41.0 39.0 41.0 35.0 41.0 16-17 38.66007487559844 41.0 39.0 41.0 35.0 41.0 18-19 38.59992989635668 41.0 39.0 41.0 35.0 41.0 20-21 38.51188489391485 40.5 39.0 41.0 34.0 41.0 22-23 38.40179485928606 40.0 38.5 41.0 34.0 41.0 24-25 38.28720734665812 40.0 38.0 41.0 34.0 41.0 26-27 38.14737054335647 40.0 38.0 41.0 34.0 41.0 28-29 37.98722330140012 40.0 38.0 41.0 33.0 41.0 30-31 37.83273027070574 40.0 38.0 41.0 33.0 41.0 32-33 37.91734945960184 40.0 38.0 41.0 33.0 41.0 34-35 37.969829391898635 40.0 38.0 41.0 33.5 41.0 36-37 38.076474978861555 40.0 38.0 41.0 34.0 41.0 38-39 38.01392382545292 40.0 38.0 41.0 33.0 41.0 40-41 37.88816437655066 40.0 38.0 41.0 33.0 41.0 42-43 37.65650858277736 40.0 38.0 41.0 33.0 41.0 44-45 37.6258367631399 40.0 38.0 41.0 33.0 41.0 46-47 37.46646544131702 40.0 38.0 41.0 32.5 41.0 48-49 37.29114053488837 40.0 37.5 41.0 32.0 41.0 50-51 36.39351378684731 39.0 36.0 40.5 31.0 40.5 52-53 36.50174295573835 39.0 36.5 40.5 31.0 41.0 54-55 36.830375774393815 40.0 36.5 41.0 31.0 41.0 56-57 36.68899685297488 40.0 36.0 41.0 31.0 41.0 58-59 36.448157302529395 39.0 35.5 41.0 31.0 41.0 60-61 36.1593426762034 39.0 35.0 41.0 30.5 41.0 62-63 35.83175791106652 39.0 35.0 41.0 30.0 41.0 64-65 35.456437709463856 38.0 35.0 40.0 29.0 41.0 66-67 35.08553263693807 37.0 35.0 40.0 29.0 41.0 68-69 34.69038139125003 37.0 34.5 39.0 29.0 41.0 70-71 34.2520717582606 36.0 34.0 39.0 28.0 41.0 72-73 33.80271278646126 35.5 34.0 38.5 28.0 40.0 74-75 33.33373118915952 35.0 34.0 37.0 26.5 39.5 76-77 32.822556127908534 35.0 33.5 37.0 26.0 39.0 78-79 32.33229129828834 35.0 33.0 36.0 25.0 38.5 80-81 31.929978705598785 35.0 33.0 36.0 24.5 37.0 82-83 31.516541832529143 35.0 32.5 35.0 23.0 37.0 84-85 31.15391381048139 35.0 32.0 35.0 20.5 36.5 86-87 30.796738062545714 35.0 32.0 35.0 19.0 36.0 88-89 30.418824332850754 34.5 31.5 35.0 16.0 36.0 90-91 30.07781637509766 34.0 31.0 35.0 7.5 35.0 92-93 29.703020098981902 34.0 31.0 35.0 2.0 35.0 94-95 29.265829815275104 34.0 30.5 35.0 2.0 35.0 96-97 28.78824666181601 34.0 30.0 35.0 2.0 35.0 98-99 28.23069451622728 34.0 29.0 35.0 2.0 35.0 100-101 26.249802301823095 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6612128127834218 1101 2 1.8834444627387334 1101 3 1.0393992296797592 1101 4 0.5852559367751624 1101 5 0.420175227915081 1101 6 0.3511963144392709 1101 7 0.33877434502863224 1101 8 0.32194352909640855 1101 9 0.37280752799506445 1101 10-11 0.36045306531841703 1101 12-13 0.40333428457316245 1101 14-15 0.5308386108947616 1101 16-17 0.4882815341328879 1101 18-19 0.4848735431257367 1101 20-21 0.4641220150100267 1101 22-23 0.47706906525429815 1101 24-25 0.4977377594667587 1101 26-27 0.5781126038340929 1101 28-29 0.5617936896630624 1101 30-31 0.6038650635351885 1101 32-33 0.5948758085415236 1101 34-35 0.637642598489073 1101 36-37 0.6505833276216961 1101 38-39 0.6731257616846662 1101 40-41 0.699759645240313 1101 42-43 0.7270038893223116 1101 44-45 0.6567138584722372 1101 46-47 0.5880218593265383 1101 48-49 0.5487720749446581 1101 50-51 0.5397657143360064 1101 52-53 0.5741412816478615 1101 54-55 0.6846593776361161 1101 56-57 0.7236981678583305 1101 58-59 0.7116615394121766 1101 60-61 0.7457153963277747 1101 62-63 0.7934262734998683 1101 64-65 0.7744323852790629 1101 66-67 0.6903595616271474 1101 68-69 0.6828689746011634 1101 70-71 0.5968455221407396 1101 72-73 0.5618505587584011 1101 74-75 0.5026423512682001 1101 76-77 0.5650164309231371 1101 78-79 0.6146206656540016 1101 80-81 0.5374922239473428 1101 82-83 0.6017078444002131 1101 84-85 0.5419265017640278 1101 86-87 0.4213178240854205 1101 88-89 0.4626706724680254 1101 90-91 0.4162252719570496 1101 92-93 0.3287947453585751 1101 94-95 0.26657386752448886 1101 96-97 0.18416301641196853 1101 98-99 0.08234630591054426 1101 100-101 0.05858820071414783 1102 1 0.9217544188451576 1102 2 2.712662083475074 1102 3 1.5254475609093987 1102 4 0.7986103437369181 1102 5 0.5465665669644295 1102 6 0.390366818196874 1102 7 0.3129151097144671 1102 8 0.3373098700078003 1102 9 0.3434607963402385 1102 10-11 0.32909713769642224 1102 12-13 0.3753943636079313 1102 14-15 0.4372278201524864 1102 16-17 0.4200851824431453 1102 18-19 0.42565068243444415 1102 20-21 0.426930815808781 1102 22-23 0.44946887843225625 1102 24-25 0.4788925098827974 1102 26-27 0.5281841927745532 1102 28-29 0.521168869409486 1102 30-31 0.5073798115203019 1102 32-33 0.5152029758486947 1102 34-35 0.5208630158129495 1102 36-37 0.5505833846369868 1102 38-39 0.5621322468077921 1102 40-41 0.5279527407326867 1102 42-43 0.690542716820822 1102 44-45 0.5278549434050603 1102 46-47 0.4424416328753651 1102 48-49 0.5056818261104965 1102 50-51 0.4373553313138103 1102 52-53 0.48905220855223774 1102 54-55 0.6007487211298894 1102 56-57 0.6229979709762148 1102 58-59 0.6166495125775526 1102 60-61 0.6429992740094619 1102 62-63 0.6953161169028803 1102 64-65 0.6688368451033497 1102 66-67 0.6227696632767987 1102 68-69 0.6361682574316205 1102 70-71 0.5719127832252582 1102 72-73 0.5746656256208809 1102 74-75 0.5907390870899363 1102 76-77 0.6691902844123305 1102 78-79 0.6488463996870735 1102 80-81 0.6221510417614162 1102 82-83 0.5810348385681756 1102 84-85 0.5019235206550299 1102 86-87 0.4673185495446539 1102 88-89 0.42274942090597634 1102 90-91 0.4288863142996959 1102 92-93 0.3224934070686132 1102 94-95 0.2739177137644546 1102 96-97 0.2389048164863361 1102 98-99 0.20093108425863093 1102 100-101 0.20332358540299467 1103 1 0.8531017374523664 1103 2 2.68269258375544 1103 3 1.4512687567725422 1103 4 0.7900709040205456 1103 5 0.4859798704070286 1103 6 0.4065452995542955 1103 7 0.31073400489983527 1103 8 0.31776724165734294 1103 9 0.23925588842137557 1103 10-11 0.2731598400873807 1103 12-13 0.30581941604614826 1103 14-15 0.3405297280134221 1103 16-17 0.3616994472288724 1103 18-19 0.3616338967828696 1103 20-21 0.41907065939063415 1103 22-23 0.3210277674771973 1103 24-25 0.39076814620104017 1103 26-27 0.37208204701455116 1103 28-29 0.3702711360822164 1103 30-31 0.40201563359369885 1103 32-33 0.4223825844465594 1103 34-35 0.40582167379124456 1103 36-37 0.43036419320795716 1103 38-39 0.4118881051756489 1103 40-41 0.45459238258410295 1103 42-43 0.5140773277634452 1103 44-45 0.3939942247697843 1103 46-47 0.3352285271105089 1103 48-49 0.36508452154104276 1103 50-51 0.3197719889530717 1103 52-53 0.36016932294846526 1103 54-55 0.5251312908699433 1103 56-57 0.5659525466365096 1103 58-59 0.5389389201386265 1103 60-61 0.5415216415644082 1103 62-63 0.5020110173713022 1103 64-65 0.4821876270499885 1103 66-67 0.44264004316023886 1103 68-69 0.40020591806253236 1103 70-71 0.3508074593266173 1103 72-73 0.4672016212458914 1103 74-75 0.4318646781988278 1103 76-77 0.5114811533524772 1103 78-79 0.5010570509009327 1103 80-81 0.4190002135787303 1103 82-83 0.44450824710254366 1103 84-85 0.39710305651408007 1103 86-87 0.3656370426645914 1103 88-89 0.3709944283633746 1103 90-91 0.3148270422032162 1103 92-93 0.1163101309305894 1103 94-95 0.11609704448608582 1103 96-97 0.022839388547399864 1103 98-99 -0.01109541829697136 1103 100-101 0.03864930815347378 1104 1 0.8610756256841334 1104 2 2.6437656963327782 1104 3 1.4359389027659901 1104 4 0.8701681756827213 1104 5 0.5368709517825394 1104 6 0.39216616131365356 1104 7 0.31431569340104915 1104 8 0.24899729018479633 1104 9 0.3177790319239904 1104 10-11 0.3223666918572192 1104 12-13 0.2955918228545684 1104 14-15 0.3196891174308121 1104 16-17 0.3416687237643501 1104 18-19 0.37084843409033397 1104 20-21 0.3724882402681615 1104 22-23 0.40475353411564896 1104 24-25 0.39689160624391207 1104 26-27 0.39103810322940546 1104 28-29 0.3888709817361402 1104 30-31 0.341413100897924 1104 32-33 0.40191106652951447 1104 34-35 0.4274467156010999 1104 36-37 0.3926009556028234 1104 38-39 0.42423238848478917 1104 40-41 0.38387745006846785 1104 42-43 0.5439460868845387 1104 44-45 0.421171345299868 1104 46-47 0.4111108784029227 1104 48-49 0.43259640488083306 1104 50-51 0.3899371728883665 1104 52-53 0.4371966511730534 1104 54-55 0.40537729347476414 1104 56-57 0.5291171494382922 1104 58-59 0.5624892685384566 1104 60-61 0.5523969015329087 1104 62-63 0.6196659062738874 1104 64-65 0.5730508981029985 1104 66-67 0.4939944505254914 1104 68-69 0.5153635547363606 1104 70-71 0.357516434756441 1104 72-73 0.3150871043061301 1104 74-75 0.4183779031909367 1104 76-77 0.48891553291302614 1104 78-79 0.5072411880110721 1104 80-81 0.4361836350163877 1104 82-83 0.49437422948915 1104 84-85 0.48410447104167176 1104 86-87 0.45706965079559936 1104 88-89 0.4315499789360082 1104 90-91 0.3032550442437234 1104 92-93 0.11537096682857495 1104 94-95 0.09381515735305257 1104 96-97 0.07970755532337748 1104 98-99 0.03698005431780871 1104 100-101 -0.014451874481284932 1105 1 0.7105074465946437 1105 2 2.391357804228882 1105 3 1.2256560283985891 1105 4 0.6412490213588384 1105 5 0.4307488082679143 1105 6 0.31274412731052337 1105 7 0.2124650583435468 1105 8 0.14723074248908574 1105 9 0.10771893521915388 1105 10-11 0.08826966685876414 1105 12-13 0.09832372708113013 1105 14-15 -0.021667173294908082 1105 16-17 0.04992444946898189 1105 18-19 0.030399375125121253 1105 20-21 0.06112297760430607 1105 22-23 0.09851136091285184 1105 24-25 0.035393921993552624 1105 26-27 0.09093257926603115 1105 28-29 0.12552297168000592 1105 30-31 0.19533148027338143 1105 32-33 0.19148926901299035 1105 34-35 0.16787492736212073 1105 36-37 0.27105063670972385 1105 38-39 0.10467722707358007 1105 40-41 0.11891580819651182 1105 42-43 0.17946195513602703 1105 44-45 0.0709064984886183 1105 46-47 -0.049238230406388084 1105 48-49 0.05296827461672393 1105 50-51 -0.008689349266788327 1105 52-53 0.08097099928861695 1105 54-55 0.1446439048399455 1105 56-57 0.14994931307246873 1105 58-59 0.13407145127347775 1105 60-61 0.13897899694979543 1105 62-63 0.17209372874157935 1105 64-65 0.28049304802095776 1105 66-67 0.06151115193750201 1105 68-69 0.05243533387969279 1105 70-71 -0.021744177125370356 1105 72-73 -0.013125169712722595 1105 74-75 0.014454051879162932 1105 76-77 -0.040416519754359115 1105 78-79 -0.05052951821070906 1105 80-81 0.03400818014349127 1105 82-83 0.03331166604707292 1105 84-85 -0.03527330722120681 1105 86-87 -0.014836802378230374 1105 88-89 -0.03994502755827867 1105 90-91 -0.20202521421795083 1105 92-93 -0.22402207027799292 1105 94-95 -0.4443738318304149 1105 96-97 -0.5333280413687227 1105 98-99 -0.47573497931786335 1105 100-101 -0.2879539719355968 1106 1 0.624452674864461 1106 2 2.416649948692445 1106 3 1.2232315008782635 1106 4 0.6499952993786948 1106 5 0.33983866166828136 1106 6 0.20772602221777703 1106 7 0.09220938440949311 1106 8 0.056244185366118415 1106 9 0.013391550552903198 1106 10-11 0.016820740540779866 1106 12-13 0.038292766719443705 1106 14-15 -0.001768408649667208 1106 16-17 -0.016102761580910396 1106 18-19 -0.03547695090718861 1106 20-21 0.022255751445506178 1106 22-23 0.04320112477635263 1106 24-25 0.0674127190109246 1106 26-27 -0.09483671048147357 1106 28-29 -0.05682996212873803 1106 30-31 -0.0065868991020749945 1106 32-33 0.027293083419728248 1106 34-35 -0.015289002232982796 1106 36-37 0.03710964909512171 1106 38-39 0.06074157874807895 1106 40-41 0.08254709736106491 1106 42-43 -0.04039320770066723 1106 44-45 0.038660167952627944 1106 46-47 0.03566378451320418 1106 48-49 -0.1461273904495215 1106 50-51 -0.12887554444893112 1106 52-53 -0.020600666263611345 1106 54-55 0.17450645393179087 1106 56-57 0.1937490421357566 1106 58-59 0.10635215974561163 1106 60-61 0.13069049318715997 1106 62-63 0.16660877761020743 1106 64-65 0.04968924882332004 1106 66-67 0.05377189712261554 1106 68-69 0.1555980306429703 1106 70-71 0.04943595785628929 1106 72-73 0.10181388026241223 1106 74-75 0.030930312530223603 1106 76-77 0.1190595964997101 1106 78-79 0.14641803848300583 1106 80-81 0.08243251728166356 1106 82-83 0.07392862591240146 1106 84-85 -0.13707386974322588 1106 86-87 -0.07547779083380846 1106 88-89 -0.001950766220478073 1106 90-91 -0.12389547736581719 1106 92-93 -0.11229321598314712 1106 94-95 -0.11714311388390897 1106 96-97 -0.16507386544165215 1106 98-99 -0.2034441881064346 1106 100-101 -0.3186615118384637 1107 1 0.7549804638116591 1107 2 2.5103936956422928 1107 3 1.346710314448849 1107 4 0.7232712206324763 1107 5 0.4598723903002906 1107 6 0.27551265577667294 1107 7 0.20521526393811484 1107 8 0.14237374766482702 1107 9 0.12598958907499025 1107 10-11 0.15173205967313663 1107 12-13 0.1648036772870327 1107 14-15 0.16534337099702867 1107 16-17 0.14048651325950345 1107 18-19 0.17894718964169698 1107 20-21 0.1654814870811876 1107 22-23 0.22550279262513584 1107 24-25 0.19288586786582584 1107 26-27 0.22409567009432152 1107 28-29 0.26644031567627025 1107 30-31 0.19423310832591056 1107 32-33 0.2169552525638636 1107 34-35 0.20788710604096394 1107 36-37 0.23808295320703365 1107 38-39 0.21869597306178434 1107 40-41 0.2590080727112607 1107 42-43 0.31336866585915857 1107 44-45 0.20043254873766614 1107 46-47 0.13640642841364325 1107 48-49 0.16035779401818928 1107 50-51 0.12660509498871875 1107 52-53 0.18460528960292777 1107 54-55 0.2983013606613909 1107 56-57 0.369769514623016 1107 58-59 0.4173893203250927 1107 60-61 0.39528494823504445 1107 62-63 0.4445515728830216 1107 64-65 0.35430522804143294 1107 66-67 0.4364463961861418 1107 68-69 0.35087347527787216 1107 70-71 0.3055332261818222 1107 72-73 0.343572125413111 1107 74-75 0.34888590396511887 1107 76-77 0.3746028566946009 1107 78-79 0.37190407268379744 1107 80-81 0.3776437409959108 1107 82-83 0.3749038080296039 1107 84-85 0.40456670079649726 1107 86-87 0.4084958025252199 1107 88-89 0.40383575225699175 1107 90-91 0.34543017724123004 1107 92-93 0.24632324465604327 1107 94-95 0.13706491542052035 1107 96-97 0.3069482428825516 1107 98-99 0.3644158728867204 1107 100-101 0.3841602331303058 1108 1 0.7408579016704415 1108 2 2.5201549396380756 1108 3 1.305174920140665 1108 4 0.6778894281783181 1108 5 0.3928773124592553 1108 6 0.30675980166341077 1108 7 0.21170757643850635 1108 8 0.16453756962345523 1108 9 0.17432090409799628 1108 10-11 0.19584442863332185 1108 12-13 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pass #Quality Count 2 137778.0 3 34930.0 4 29306.0 5 24562.0 6 22533.0 7 21845.0 8 22317.0 9 24919.0 10 27894.0 11 32240.0 12 35373.0 13 38528.0 14 41607.0 15 46792.0 16 54708.0 17 62896.0 18 60657.0 19 56248.0 20 59252.0 21 64490.0 22 68870.0 23 77483.0 24 89957.0 25 105529.0 26 124285.0 27 150440.0 28 181671.0 29 221764.0 30 270610.0 31 336165.0 32 426503.0 33 566422.0 34 801483.0 35 1230238.0 36 2226430.0 37 4313804.0 38 4551660.0 39 637889.0 40 51.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.417294161071744 18.692343958478155 14.146851521061258 24.743510359388843 2 17.154203749371895 21.56449365739173 37.20253189442256 24.078770698813816 3 18.384727026580062 25.079856420231593 29.448294780417783 27.087121772770562 4 13.758316923601638 17.492007961879487 36.4663793743189 32.28329574019998 5 15.23747231084514 36.397393338070096 33.560922855667215 14.80421149541755 6 32.017617703361054 35.07606605481224 18.56368837384952 14.342627867977189 7 28.754262744708086 31.288902121628194 21.91283748453292 18.043997649130805 8 26.736235093875237 33.647500070470606 20.886468700314246 18.729796135339914 9 27.222301866269344 16.25870812159576 19.851365880617543 36.66762413151736 10-11 25.471678184253495 25.51570146215853 27.713531707496063 21.299088646091914 12-13 26.47517145826759 22.772776147892294 27.554448028365954 23.19760436547417 14-15 23.64837051926802 24.177466301723793 24.901218862121397 27.27294431688679 16-17 23.837463458547802 26.997483616474717 25.618129538413342 23.546923386564135 18-19 24.075507169054827 26.31580333625734 26.602338609688143 23.006350884999694 20-21 24.70170765771374 25.688835056973886 26.190684956536458 23.41877232877592 22-23 24.628069071809534 25.594948672618727 26.000847537762713 23.77613471780903 24-25 24.39095506283869 25.746432219226083 26.028883941965425 23.833728775969803 26-27 24.055943056222205 26.099316378096336 25.877074001247745 23.967666564433706 28-29 24.117273743078847 26.02234460892159 25.928272037593835 23.93210961040573 30-31 24.159859017280247 25.694985666212748 25.977424567247194 24.16773074925981 32-33 23.995707446819132 25.820858755498215 26.042971204657867 24.140462593024782 34-35 24.366537630970374 25.775634524553997 25.868681348879978 23.98914649559565 36-37 24.203195973957513 25.544594636130295 25.97860039084453 24.273608999067662 38-39 24.089690407475132 25.595618719866742 26.17162379078103 24.1430670818771 40-41 24.292411688123924 25.27676084045794 26.37877755381813 24.05204991760001 42-43 24.043430972138484 25.31859396995264 26.32053441839865 24.317440639510227 44-45 23.714540742728605 25.496414625297025 26.286077128438105 24.50296750353626 46-47 23.828239670217428 25.39568966155892 26.275876546376715 24.50019412184694 48-49 23.680428799508842 25.465156509482544 26.32042197479416 24.53399271621445 50-51 23.410877800723632 25.640507950451546 26.401424709988238 24.54718953883659 52-53 23.55673122783659 25.537580240602647 26.28112572251161 24.62456280904915 54-55 23.41956330253575 25.351736543225044 26.50375987175605 24.72494028248315 56-57 23.379942287446763 25.540515084638933 26.455263307699873 24.624279320214434 58-59 23.6112110791416 25.227744055871966 26.26517634085636 24.895868524130073 60-61 23.534451042052744 25.087073467633292 26.49977465993445 24.878700830379515 62-63 23.509139926978754 25.09670313870031 26.50579692091974 24.888360013401197 64-65 23.48250989281325 25.12578204905252 26.30296988573035 25.08873817240388 66-67 23.514245506790964 25.344844091610845 26.055863463722044 25.08504693787615 68-69 23.431240498359482 25.07493441583654 26.34095453661888 25.152870549185092 70-71 23.55795095517103 25.065772425988044 26.25779653937053 25.118480079470395 72-73 23.60412506876388 25.09343610562132 26.196902476611015 25.10553634900378 74-75 23.516358667439096 25.235757640589767 26.210587814974478 25.03729587699666 76-77 23.695369000631892 25.037926451351527 26.309497613477852 24.95720693453873 78-79 23.748064427409275 25.015017030491943 26.35983678951502 24.87708175258376 80-81 23.728300679383743 25.11669428819769 26.42757146054626 24.72743357187231 82-83 23.76452890016525 25.050622218686424 26.438618354991828 24.7462305261565 84-85 23.867194836682774 24.910875650746807 26.441931057953905 24.779998454616514 86-87 23.9239490037838 24.857762679630284 26.49381347788565 24.724474838700267 88-89 24.042416678805317 24.623497521703634 26.554007631881106 24.78007816760994 90-91 24.144257694609898 24.60686052438006 26.641824462242695 24.607057318767346 92-93 24.29711788939894 24.47743758975822 26.58801028420029 24.637434236642548 94-95 24.300507779634252 24.41860434818068 26.482409268433866 24.7984786037512 96-97 24.55785622790483 24.2692155260448 26.333583485519625 24.83934476053075 98-99 24.51756635540346 24.087216478509497 26.45480973005631 24.940407436030732 100-101 24.49576899775645 24.016624205062865 26.70049464749833 24.787112149682358 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 663.0 1 475.5 2 398.5 3 488.0 4 436.0 5 465.5 6 713.5 7 1364.5 8 2316.5 9 2914.0 10 2769.0 11 2560.5 12 2733.0 13 2991.5 14 3220.0 15 3499.5 16 3749.0 17 4014.0 18 4410.0 19 4824.0 20 5386.5 21 6200.0 22 7191.5 23 8492.5 24 10220.0 25 12691.0 26 16383.5 27 21047.5 28 26732.0 29 35079.5 30 48335.5 31 68241.5 32 94522.5 33 128813.5 34 173823.0 35 223345.5 36 275131.5 37 323152.0 38 365633.0 39 398823.5 40 430630.0 41 483098.5 42 540050.0 43 592611.5 44 645211.5 45 685657.5 46 718997.5 47 761914.0 48 806044.5 49 834688.5 50 848181.5 51 837818.5 52 808083.5 53 790410.0 54 770761.5 55 717318.0 56 647213.0 57 575414.0 58 505304.5 59 435071.0 60 355700.0 61 279857.5 62 219655.0 63 171277.5 64 132835.5 65 102570.0 66 76517.0 67 57579.5 68 42482.0 69 30186.0 70 22043.0 71 15965.5 72 11918.5 73 8985.0 74 6807.0 75 5136.5 76 3605.0 77 2274.0 78 1417.0 79 976.5 80 680.0 81 495.5 82 405.0 83 329.5 84 274.0 85 242.5 86 198.0 87 182.0 88 173.5 89 148.5 90 124.5 91 102.5 92 88.5 93 87.0 94 81.5 95 72.5 96 65.0 97 58.5 98 51.5 99 41.5 100 29.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03551478116858965 2 1.0940485455866678 3 0.025671104654369187 4 0.0278412273426894 5 0.03075787223579176 6 0.011886485338159223 7 0.018026485797646534 8 0.019803092905151344 9 0.010775382521739277 10-11 0.010242979088871385 12-13 0.02433430907836394 14-15 0.014693177348386693 16-17 0.02629031299476989 18-19 0.030627664874492547 20-21 0.04302340567017758 22-23 0.031148494319689395 24-25 0.009264977130668412 26-27 0.015023035997011365 28-29 0.023755609694811886 30-31 0.018043846779153095 32-33 0.029487627088894994 34-35 0.02556983226224758 36-37 0.02434009607219946 38-39 0.011472715278919503 40-41 0.012818191345678033 42-43 0.01103290374741994 44-45 0.017734242608952747 46-47 0.013431612692243211 48-49 0.010133026205996496 50-51 0.024259078158502173 52-53 0.015268983235020987 54-55 0.015882404581586167 56-57 0.019088399166464556 58-59 0.021831434244501298 60-61 0.016831471570611538 62-63 0.023500981966048978 64-65 0.019209926037010487 66-67 0.015109840904544173 68-69 0.014586117962429564 70-71 0.01592580703535257 72-73 0.020931556703077853 74-75 0.027551877650913367 76-77 0.02463812625472877 78-79 0.022630039393803137 80-81 0.018217456594218712 82-83 0.016686796724723525 84-85 0.016573950344930876 86-87 0.023058276937631655 88-89 0.02342285754926945 90-91 0.01859650469044531 92-93 0.03843721305552754 94-95 0.057525612221992095 96-97 0.0731504955778976 98-99 0.0793570464664934 100-101 0.07045954344438055 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7280129E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.62420435471467 #Duplication Level Percentage of deduplicated Percentage of total 1 74.42961521544753 30.980735137705683 2 12.448492867415125 10.363172220429902 3 4.331120295041025 5.408383087369191 4 2.166426347635244 3.6070309205363014 5 1.3578751344122109 2.8260236041479754 6 0.9151027183937467 2.2854253533585736 7 0.6790640163695094 1.9785849571108436 8 0.5014191085236032 1.6696937152436249 9 0.39410328241053444 1.4763812007527921 >10 2.43487382942064 19.17340417918498 >50 0.208220528257245 5.984410942699796 >100 0.12197333737453932 9.629448589973 >500 0.00878406088214818 2.460940719747581 >1k 0.002872940795921671 1.9808686233797077 >5k 5.631762091874441E-5 0.17549674836013227 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.786993835520556E-6 0.0 0.0 0.0 0.0 3 5.786993835520556E-6 0.0 0.0 5.786993835520556E-6 0.0 4 5.786993835520556E-6 0.0 0.0 1.736098150656167E-5 0.0 5 5.786993835520556E-6 0.0 0.0 2.3147975342082226E-5 0.0 6 1.1573987671041113E-5 0.0 0.0 2.3147975342082226E-5 0.0 7 2.3147975342082226E-5 0.0 0.0 3.472196301312334E-5 0.0 8 2.3147975342082226E-5 0.0 0.0 6.944392602624668E-5 0.0 9 2.3147975342082226E-5 0.0 0.0 1.6782282123009614E-4 5.786993835520556E-6 10-11 2.3147975342082226E-5 0.0 0.0 3.356456424601923E-4 5.786993835520556E-6 12-13 2.8934969177602784E-5 0.0 0.0 4.542790160883637E-4 1.4467484588801392E-5 14-15 4.629595068416445E-5 0.0 0.0 5.758058866342954E-4 2.0254478424321948E-5 16-17 5.208294451968501E-5 2.893496917760278E-6 0.0 7.69670180124234E-4 2.3147975342082226E-5 18-19 6.65504291084864E-5 1.1573987671041113E-5 0.0 0.0010329783996404194 2.3147975342082226E-5 20-21 7.523091986176724E-5 1.1573987671041113E-5 0.0 0.0014120264958670157 5.208294451968501E-5 22-23 8.101791369728779E-5 1.1573987671041113E-5 0.0 0.002152761706813647 5.7869938355205574E-5 24-25 8.680490753280835E-5 1.1573987671041113E-5 0.0 0.003836776912950129 6.365693219072613E-5 26-27 8.680490753280835E-5 1.1573987671041113E-5 0.0 0.007534665973847765 7.233742294400696E-5 28-29 1.0995288287489058E-4 1.1573987671041113E-5 0.0 0.026151425142717392 7.523091986176724E-5 30-31 1.2731386438145226E-4 1.1573987671041113E-5 0.0 0.0880693656858696 7.523091986176724E-5 32-33 1.2731386438145226E-4 1.4467484588801392E-5 0.0 0.22605734019693952 8.680490753280835E-5 34-35 1.5046183972353448E-4 2.0254478424321948E-5 0.0 0.4019588048214223 9.25919013683289E-5 36-37 1.9097079657217836E-4 3.761545993088362E-5 0.0 0.6787854419373838 9.25919013683289E-5 38-39 2.2569275958530171E-4 4.0508956848643896E-5 0.0 1.1084784147155382 9.25919013683289E-5 40-41 2.2569275958530171E-4 4.0508956848643896E-5 0.0 1.5775837090105056 9.25919013683289E-5 42-43 2.401602441741031E-4 4.629595068416445E-5 0.0 2.0456155159489837 1.0127239212160975E-4 44-45 2.517342318451442E-4 4.918944760192473E-5 0.0 2.5657215869163936 1.1573987671041113E-4 46-47 3.1828466095363064E-4 5.208294451968501E-5 0.0 3.145430800892748 1.331008582169728E-4 48-49 3.472196301312334E-4 5.208294451968501E-5 0.0 3.7292950764430057 1.5624883355905503E-4 50-51 3.501131270489937E-4 5.208294451968501E-5 0.0 4.309157645756001 1.5624883355905503E-4 52-53 3.5590012088451424E-4 5.208294451968501E-5 0.0 4.864555698629333 1.7650331198337696E-4 54-55 3.645806116377951E-4 5.208294451968501E-5 0.0 5.43551497792638 1.9097079657217836E-4 56-57 3.761545993088362E-4 5.208294451968501E-5 0.0 6.060186240507811 1.9675779040769891E-4 58-59 3.761545993088362E-4 5.208294451968501E-5 0.0 6.6973545162770485 2.0254478424321947E-4 60-61 3.819415931443567E-4 5.208294451968501E-5 0.0 7.332780906901794 2.0254478424321947E-4 62-63 3.819415931443567E-4 5.208294451968501E-5 0.0 7.947050048063877 2.1990576574978116E-4 64-65 3.877285869798773E-4 5.208294451968501E-5 0.0 8.56339672001291 2.1990576574978116E-4 66-67 4.0508956848643894E-4 5.208294451968501E-5 0.0 9.213843253137751 2.2569275958530171E-4 68-69 4.3402453766404174E-4 5.208294451968501E-5 0.0 9.89720910069595 2.2569275958530171E-4 70-71 4.5138551917060343E-4 5.7869938355205574E-5 0.0 10.585962639515017 2.2569275958530171E-4 72-73 4.687465006771651E-4 6.65504291084864E-5 0.0 11.250341360298872 2.4594723800962367E-4 74-75 4.7453349451268565E-4 6.944392602624668E-5 0.0 11.909048827123918 2.546277287629045E-4 76-77 4.7453349451268565E-4 6.944392602624668E-5 0.0 12.590788529414336 2.546277287629045E-4 78-79 4.803204883482062E-4 7.523091986176724E-5 0.0 13.29741519869441 2.546277287629045E-4 80-81 5.208294451968501E-4 7.523091986176724E-5 0.0 14.011385563151757 2.575212256806648E-4 82-83 5.266164390323706E-4 8.101791369728779E-5 0.0 14.718666162735243 2.6041472259842504E-4 84-85 5.497644143744528E-4 8.391141061504807E-5 0.0 15.420125046520196 2.662017164339456E-4 86-87 5.61338402045494E-4 8.680490753280835E-5 0.0 16.120643543806878 2.748822071872264E-4 88-89 5.671253958810145E-4 8.680490753280835E-5 0.0 16.84490318330378 2.8356269794050726E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15650 0.0 43.981716 1 GTATCAA 25940 0.0 42.59638 1 ATCAACG 31850 0.0 36.679787 3 TCAACGC 32650 0.0 35.780533 4 CAACGCA 33715 0.0 34.62041 5 AACGCAG 35295 0.0 33.326817 6 TATCAAC 33275 0.0 32.80223 2 ACGCAGA 41740 0.0 27.965528 7 CGCAGAG 42890 0.0 27.050316 8 GTACATG 57435 0.0 24.018589 1 ACATGGG 61445 0.0 23.16335 3 TACATGG 58960 0.0 23.063229 2 GCAGAGT 51735 0.0 21.872858 9 CATGGGG 48245 0.0 21.44864 4 CAGAGTA 47755 0.0 18.639885 10-11 AGAGTAC 46745 0.0 18.575325 10-11 ATGGGGA 29810 0.0 18.032583 5 TAGGACG 9640 0.0 17.044878 4 TCGCCGT 11305 0.0 16.64504 94-95 CGTATCA 4250 0.0 16.603428 94-95 >>END_MODULE