FastQCFastQC Report
Fri 27 May 2016
SRR940201_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940201_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16135808
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147100.045.2423741
GTATCAA248750.043.9413531
ATCAACG299000.036.377633
TCAACGC302750.035.8639534
CAACGCA310350.034.9394575
TATCAAC317500.034.005252
AACGCAG324400.033.5750126
ACGCAGA387950.027.977067
CGCAGAG397500.027.161268
GTACATG545700.025.2704521
TACATGG560650.024.2983972
ACATGGG574700.023.4139943
CATGGGG447600.021.9020964
GCAGAGT487100.021.8233539
AGAGTAC435900.018.62241710-11
CAGAGTA449800.018.53269210-11
ATGGGGA275350.018.3362375
CGTATCA41200.016.90143894-95
TCGCCGT108950.016.88367794-95
TAGGACG92550.016.2172324
Produced by FastQC (version 0.11.5)