##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940201_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16135808 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2073636473612 34.0 31.0 34.0 30.0 34.0 2 31.826889858877845 34.0 31.0 34.0 30.0 34.0 3 32.229197075225485 34.0 31.0 34.0 30.0 34.0 4 35.74126464568741 37.0 35.0 37.0 35.0 37.0 5 35.81550604717161 37.0 35.0 37.0 35.0 37.0 6 35.89738629760592 37.0 37.0 37.0 35.0 37.0 7 35.880852201513555 37.0 37.0 37.0 35.0 37.0 8 35.883914211175544 37.0 37.0 37.0 35.0 37.0 9 37.646741148630426 39.0 39.0 39.0 35.0 39.0 10-11 37.59073474969459 39.0 38.0 39.0 35.0 39.0 12-13 37.5091921644085 39.0 38.0 39.0 35.0 39.0 14-15 38.949337399155965 41.0 39.0 41.0 36.0 41.0 16-17 38.88511591734358 41.0 39.0 41.0 36.0 41.0 18-19 38.8143975498469 41.0 39.0 41.0 36.0 41.0 20-21 38.742420831978166 41.0 39.0 41.0 35.0 41.0 22-23 38.63968414225057 40.5 39.0 41.0 35.0 41.0 24-25 38.53524524461372 40.0 39.0 41.0 35.0 41.0 26-27 38.38196615254718 40.0 38.0 41.0 34.0 41.0 28-29 38.23030049068507 40.0 38.0 41.0 34.0 41.0 30-31 38.087491001380286 40.0 38.0 41.0 34.0 41.0 32-33 38.16559356680496 40.0 38.0 41.0 34.0 41.0 34-35 38.24522069176827 40.0 38.0 41.0 34.5 41.0 36-37 38.37644687517353 41.0 39.0 41.0 34.5 41.0 38-39 38.30806690932366 41.0 38.0 41.0 34.0 41.0 40-41 38.18512165613275 40.0 38.0 41.0 34.0 41.0 42-43 38.06764371514584 40.0 38.0 41.0 33.5 41.0 44-45 37.94477785060407 40.0 38.0 41.0 33.0 41.0 46-47 37.76045717698178 40.0 38.0 41.0 33.0 41.0 48-49 37.599574220268366 40.0 38.0 41.0 33.0 41.0 50-51 36.69004527074195 39.0 36.5 40.5 31.5 40.5 52-53 36.694596359847615 39.5 36.5 40.5 31.5 41.0 54-55 37.10545737777743 40.0 37.0 41.0 32.0 41.0 56-57 37.00529775143581 40.0 36.5 41.0 32.0 41.0 58-59 36.767688887968916 39.5 36.0 41.0 31.5 41.0 60-61 36.482595386608466 39.0 35.5 41.0 31.0 41.0 62-63 36.16296714115587 39.0 35.0 41.0 31.0 41.0 64-65 35.801389431505385 38.0 35.0 40.5 30.5 41.0 66-67 35.4085871931545 37.0 35.0 40.0 30.0 41.0 68-69 34.96988229532727 37.0 35.0 39.5 29.0 41.0 70-71 34.54331509150332 36.0 34.5 39.0 29.0 41.0 72-73 34.11106977103347 36.0 34.0 38.5 29.0 40.0 74-75 33.66185848889624 35.0 34.0 37.0 28.0 39.5 76-77 33.1263980706761 35.0 34.0 37.0 27.0 39.0 78-79 32.671248009396244 35.0 33.5 36.5 26.0 39.0 80-81 32.28736351473691 35.0 33.0 36.0 26.0 37.0 82-83 31.890331398340884 35.0 33.0 35.5 25.0 37.0 84-85 31.554098065619023 35.0 33.0 35.0 24.0 36.5 86-87 31.20406393655651 35.0 32.5 35.0 22.5 36.0 88-89 30.852515132802772 35.0 32.0 35.0 19.5 36.0 90-91 30.494113681818725 35.0 32.0 35.0 17.5 35.5 92-93 30.13307096242097 34.0 31.0 35.0 9.0 35.0 94-95 29.704955215133943 34.0 31.0 35.0 2.0 35.0 96-97 29.239154153296816 34.0 30.5 35.0 2.0 35.0 98-99 28.725549535542317 34.0 30.0 35.0 2.0 35.0 100-101 26.775245497467495 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.09757544911937543 1101 2 0.016879295046479115 1101 3 0.06286368936376618 1101 4 0.09921286302999022 1101 5 0.15889975159814185 1101 6 0.23051420947275147 1101 7 0.22907037821062914 1101 8 0.23545946972887322 1101 9 0.3577352961481921 1101 10-11 0.39660216180757857 1101 12-13 0.3957854475346565 1101 14-15 0.4593325301448701 1101 16-17 0.48380719673139083 1101 18-19 0.48265568658346325 1101 20-21 0.5344296907878956 1101 22-23 0.5306550619552368 1101 24-25 0.5103662828362161 1101 26-27 0.5432207480096451 1101 28-29 0.5254875608296032 1101 30-31 0.5448774669062075 1101 32-33 0.5137006712664416 1101 34-35 0.5178682366097718 1101 36-37 0.5384056170260152 1101 38-39 0.5919774158273228 1101 40-41 0.6199087026785648 1101 42-43 0.6368559045820774 1101 44-45 0.621374320351805 1101 46-47 0.6575728934928975 1101 48-49 0.7049702058964513 1101 50-51 0.5924877701395346 1101 52-53 0.7426316124682728 1101 54-55 0.7792313914298958 1101 56-57 0.7619283034350346 1101 58-59 0.7664658959152604 1101 60-61 0.7951529474543193 1101 62-63 0.8477049932218534 1101 64-65 0.8171675373607314 1101 66-67 0.813244122524452 1101 68-69 0.7964112627276947 1101 70-71 0.7147221704226681 1101 72-73 0.836110668302112 1101 74-75 0.773875091918562 1101 76-77 0.8795236307124554 1101 78-79 0.8423935369122688 1101 80-81 0.7359434287803666 1101 82-83 0.6760550823776015 1101 84-85 0.6722024796982957 1101 86-87 0.6309298061059607 1101 88-89 0.6358281445064726 1101 90-91 0.5848979793169349 1101 92-93 0.5476112836029579 1101 94-95 0.5866083260092658 1101 96-97 0.5691721394665201 1101 98-99 0.6183133769776354 1101 100-101 0.7834502143664643 1102 1 0.22443100090141854 1102 2 0.685477090748325 1102 3 0.340453998923536 1102 4 0.28554687528163925 1102 5 0.2333501504334876 1102 6 0.19767343561841955 1102 7 0.1688381953184006 1102 8 0.17107232658855764 1102 9 0.18123636262398435 1102 10-11 0.18853186170639447 1102 12-13 0.2169513587769032 1102 14-15 0.23539094135151828 1102 16-17 0.26470787098841697 1102 18-19 0.2868474417061222 1102 20-21 0.26251915670582093 1102 22-23 0.27129311496319986 1102 24-25 0.2612252495984535 1102 26-27 0.30159052508238915 1102 28-29 0.2812257651046437 1102 30-31 0.28677228911448793 1102 32-33 0.2411174384442134 1102 34-35 0.23618211088317764 1102 36-37 0.2758649036364602 1102 38-39 0.27456195155386354 1102 40-41 0.27387976747208853 1102 42-43 0.123919926408945 1102 44-45 0.20934099759091396 1102 46-47 0.2894375409815453 1102 48-49 0.24725539911923988 1102 50-51 0.22572615875704116 1102 52-53 0.3334480171317793 1102 54-55 0.341925562084505 1102 56-57 0.3354503501784123 1102 58-59 0.30137087879727176 1102 60-61 0.3346554882013919 1102 62-63 0.3815373402283768 1102 64-65 0.37919767634126345 1102 66-67 0.2889211504752822 1102 68-69 0.32400680515409874 1102 70-71 0.2508431861789475 1102 72-73 0.25080468832601355 1102 74-75 0.2825143163078323 1102 76-77 0.3589278094061896 1102 78-79 0.3546270560719975 1102 80-81 0.25099530047371843 1102 82-83 0.1495492227298314 1102 84-85 0.00987847140488185 1102 86-87 0.050061567535049534 1102 88-89 -0.0864156446803861 1102 90-91 -0.09458548964603963 1102 92-93 -0.10241118523602566 1102 94-95 -0.1226383833040181 1102 96-97 -0.3139026192378118 1102 98-99 -0.3108615424806942 1102 100-101 -0.09778154348815704 1103 1 0.1721128623345578 1103 2 0.6494678018159199 1103 3 0.28704290414470535 1103 4 0.21266527743985364 1103 5 0.09041769242921305 1103 6 0.11663926227900134 1103 7 0.14561232809882796 1103 8 0.0978695927327351 1103 9 0.12801580607550278 1103 10-11 0.06172977941751867 1103 12-13 0.09269541262816006 1103 14-15 0.11210212062718483 1103 16-17 0.05845685711665283 1103 18-19 0.038095239906830614 1103 20-21 0.08648825339246713 1103 22-23 0.07765051011174506 1103 24-25 0.013733708975657066 1103 26-27 0.11076554606768241 1103 28-29 0.008468420297575108 1103 30-31 0.006460111387838197 1103 32-33 0.03748568380907358 1103 34-35 0.010146061754731761 1103 36-37 0.025738490771388456 1103 38-39 0.0809712276143415 1103 40-41 0.0077075186210251445 1103 42-43 0.10079888520502323 1103 44-45 0.09801579187003995 1103 46-47 0.07025339172972167 1103 48-49 0.05210018620446988 1103 50-51 -0.07457672647183955 1103 52-53 0.10482245615536101 1103 54-55 0.1550051277564748 1103 56-57 0.15096553015379754 1103 58-59 0.1413566763559757 1103 60-61 0.14814304126629452 1103 62-63 0.06788048278741599 1103 64-65 0.11759800139500953 1103 66-67 0.08511463513823969 1103 68-69 0.10746479838635281 1103 70-71 0.06487340416944676 1103 72-73 0.06039117042619324 1103 74-75 0.11170468781202914 1103 76-77 0.2077358818994739 1103 78-79 0.19326625948740883 1103 80-81 0.10080371325432225 1103 82-83 0.0037749415415078147 1103 84-85 -0.10001472159997249 1103 86-87 -0.09282596533145338 1103 88-89 -0.12190674093334408 1103 90-91 -0.1827294992119164 1103 92-93 -0.16883357413868438 1103 94-95 -0.2254705908591852 1103 96-97 -0.44546276135402607 1103 98-99 -0.5115060461876872 1103 100-101 -0.35464082085877635 1104 1 0.19409289580785583 1104 2 0.7172230096657408 1104 3 0.35101977438125687 1104 4 0.14559977770196042 1104 5 0.11959241618171745 1104 6 0.11140166980293031 1104 7 0.11436579734783692 1104 8 -0.002421358066477808 1104 9 0.07681274874589405 1104 10-11 0.07184868566095304 1104 12-13 0.11434417784479223 1104 14-15 0.06834720838163122 1104 16-17 0.08091556527634225 1104 18-19 0.11618289151471117 1104 20-21 0.1478331170021221 1104 22-23 0.09847355905926491 1104 24-25 0.08383291871193421 1104 26-27 0.10168794314603247 1104 28-29 0.05472297350871003 1104 30-31 0.041245916357063095 1104 32-33 0.033273566539101296 1104 34-35 0.09407893705378712 1104 36-37 0.10670317026628595 1104 38-39 0.14589528273244667 1104 40-41 0.10585111157877947 1104 42-43 -0.054671715803060295 1104 44-45 0.0250232878140082 1104 46-47 0.024540281829906974 1104 48-49 0.0049923149255590715 1104 50-51 -0.06261423436570368 1104 52-53 0.060271215175490056 1104 54-55 0.14051614573620697 1104 56-57 0.13085261773367307 1104 58-59 0.1214349310469629 1104 60-61 0.17833628397097812 1104 62-63 0.17834480884049242 1104 64-65 0.15812333476900875 1104 66-67 0.14977621748406023 1104 68-69 0.10585582172459596 1104 70-71 0.062107223823396396 1104 72-73 -0.021616859899310725 1104 74-75 0.02331029081238256 1104 76-77 0.11099197327066435 1104 78-79 0.03133337975648942 1104 80-81 -0.1516443341418494 1104 82-83 -0.22819522752541488 1104 84-85 -0.15692730493814366 1104 86-87 -0.09012506581639101 1104 88-89 -0.1969285007368491 1104 90-91 -0.29196301069650943 1104 92-93 -0.3435664562202838 1104 94-95 -0.36732797948812035 1104 96-97 -0.5029603518480918 1104 98-99 -0.4176652189301642 1104 100-101 -0.19254498458909097 1105 1 -0.059349096489512476 1105 2 0.49843207170369297 1105 3 0.11159106696671728 1105 4 0.0996691317279641 1105 5 -0.014634985795453304 1105 6 -0.011152885649167388 1105 7 -0.02134309940051793 1105 8 -0.03759796512487412 1105 9 0.03259676873923212 1105 10-11 -0.026595041913367368 1105 12-13 -0.15951239529658778 1105 14-15 -0.3043809810897926 1105 16-17 -0.23817165588832268 1105 18-19 -0.3632783773288111 1105 20-21 -0.20337373571393869 1105 22-23 -0.24450862981482402 1105 24-25 -0.10722564244741761 1105 26-27 -0.15461943379489185 1105 28-29 -0.0014838033633068903 1105 30-31 -0.12845000559480724 1105 32-33 -0.016520636213485318 1105 34-35 -0.10831095193243101 1105 36-37 -0.09970404672797883 1105 38-39 -0.2447340885875704 1105 40-41 -0.2544654371825672 1105 42-43 -0.14337110284303378 1105 44-45 -0.1940144307418592 1105 46-47 -0.19210128489654466 1105 48-49 -0.37924502238444546 1105 50-51 -0.41484889194750707 1105 52-53 -0.17748574942982032 1105 54-55 -0.3330229756035905 1105 56-57 -0.1593460027617013 1105 58-59 -0.2122572608903397 1105 60-61 -0.16323638714880673 1105 62-63 -0.11655555532587414 1105 64-65 -0.09422504240404095 1105 66-67 -0.17464271754812444 1105 68-69 -0.20938828826120215 1105 70-71 -0.26337737114966586 1105 72-73 -0.4041250712569706 1105 74-75 -0.38866185692525335 1105 76-77 -0.35096622798469923 1105 78-79 -0.2966136419772667 1105 80-81 -0.26638298416804673 1105 82-83 -0.3681843152772508 1105 84-85 -0.43118166155599624 1105 86-87 -0.32465872964596443 1105 88-89 -0.36253500740246736 1105 90-91 -0.5582104774622927 1105 92-93 -0.48435442664057504 1105 94-95 -0.5515433015497102 1105 96-97 -0.5302943269233324 1105 98-99 -0.5651734673828877 1105 100-101 -0.493714953317248 1106 1 -0.004594833868637238 1106 2 0.523524416944209 1106 3 0.07725384167330418 1106 4 0.04601747956672142 1106 5 0.00951803860131406 1106 6 0.0012223821960830605 1106 7 0.04448378909667383 1106 8 -0.03111377822381911 1106 9 -0.0336071335619863 1106 10-11 -0.10195293440266084 1106 12-13 -0.055645096129133265 1106 14-15 -0.03841876187389204 1106 16-17 0.00965785846641154 1106 18-19 -0.0307249359795847 1106 20-21 -0.07593645810079863 1106 22-23 -0.11498208466943538 1106 24-25 -0.1261503084805966 1106 26-27 -0.29513101318826784 1106 28-29 -0.247074141876773 1106 30-31 -0.11776382694024079 1106 32-33 -0.10822709519386109 1106 34-35 -0.14576782845110614 1106 36-37 -0.20412830579413566 1106 38-39 -0.1310169558987937 1106 40-41 -0.23124980389209782 1106 42-43 -0.21994249650126108 1106 44-45 -0.17950106378117425 1106 46-47 -0.13774963008749097 1106 48-49 -0.10335371971265062 1106 50-51 -0.19022638088211608 1106 52-53 -0.021725911389992802 1106 54-55 0.012329795316404102 1106 56-57 -0.057579507266076746 1106 58-59 -0.04802271946346082 1106 60-61 -0.09041054083285616 1106 62-63 -0.0924422097137807 1106 64-65 -0.08279137110883994 1106 66-67 -0.06674871751854283 1106 68-69 -0.3028674301981411 1106 70-71 -0.17083543195820283 1106 72-73 -0.2132889607674926 1106 74-75 -0.08424714736857908 1106 76-77 -0.0451956356949168 1106 78-79 0.026897999198467915 1106 80-81 -0.04380569495434372 1106 82-83 -0.08926780703613346 1106 84-85 -0.16453749542291618 1106 86-87 -0.21468955070511697 1106 88-89 -0.24038441850349201 1106 90-91 -0.22047721333708026 1106 92-93 -0.28489766387646753 1106 94-95 -0.3432898191116891 1106 96-97 -0.3116143615927953 1106 98-99 -0.4738191201364934 1106 100-101 -0.45828166021983563 1107 1 0.12465327306549057 1107 2 0.5906878499326247 1107 3 0.21965313652790286 1107 4 0.1253344199209394 1107 5 0.10710052723294439 1107 6 0.07044896672702095 1107 7 0.056973564962724765 1107 8 0.010252142580569057 1107 9 -0.03914394079360761 1107 10-11 -0.01603089091786103 1107 12-13 -0.0018426657599590612 1107 14-15 -0.01945804013705299 1107 16-17 -0.047308812473801254 1107 18-19 -0.011000040470449335 1107 20-21 -0.0050025530371726745 1107 22-23 -0.05721029918895937 1107 24-25 -0.01459770505960023 1107 26-27 0.04477166089193929 1107 28-29 -0.013904919649299075 1107 30-31 -0.02545167092985423 1107 32-33 0.0028926054642752774 1107 34-35 -0.043993749692909034 1107 36-37 -0.010162514456908411 1107 38-39 -0.034385895477548445 1107 40-41 0.019500786146345206 1107 42-43 0.027642444227566898 1107 44-45 -0.02908827104027978 1107 46-47 -0.054070566040614665 1107 48-49 0.0684003529505759 1107 50-51 -0.1717183492955101 1107 52-53 -0.004435539161420365 1107 54-55 0.07015792290598455 1107 56-57 0.0334196969564573 1107 58-59 0.08938815403281808 1107 60-61 0.004010270091193036 1107 62-63 0.02043483842298599 1107 64-65 -0.006765991185432085 1107 66-67 -0.009957382493340106 1107 68-69 0.14102706930291475 1107 70-71 0.09552412090177853 1107 72-73 0.07228753512740127 1107 74-75 0.07443779918403237 1107 76-77 0.19297380932883357 1107 78-79 0.1767278287821341 1107 80-81 0.12993582349159283 1107 82-83 0.03923970112418118 1107 84-85 -0.04036496764798514 1107 86-87 0.010713944361167904 1107 88-89 -0.030653359409967607 1107 90-91 0.028768689645982448 1107 92-93 -0.04876023132549889 1107 94-95 0.048096782272370575 1107 96-97 -0.036405633369717805 1107 98-99 -0.08502112235023773 1107 100-101 0.11050714857654143 1108 1 0.03623393046520107 1108 2 0.19535891303495845 1108 3 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62-63 -1.10687852042674 2316 64-65 -1.0574849496786527 2316 66-67 -1.016972513286447 2316 68-69 -1.0168834244766387 2316 70-71 -0.99012641498134 2316 72-73 -1.155841939449175 2316 74-75 -1.317112622652509 2316 76-77 -1.0602317305930455 2316 78-79 -0.9480191133868701 2316 80-81 -1.132886787553069 2316 82-83 -1.1088855249842524 2316 84-85 -0.9435056942202245 2316 86-87 -0.8363497539336926 2316 88-89 -0.8434214118163261 2316 90-91 -0.7290623704972354 2316 92-93 -0.8552619574994154 2316 94-95 -0.8722368008651351 2316 96-97 -0.677635292269489 2316 98-99 -0.6322243741309315 2316 100-101 -1.0179754985285463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97835.0 3 32387.0 4 25563.0 5 20131.0 6 17925.0 7 16881.0 8 17790.0 9 19680.0 10 22392.0 11 25782.0 12 28839.0 13 31714.0 14 35295.0 15 39218.0 16 46200.0 17 54722.0 18 52780.0 19 47601.0 20 49885.0 21 55067.0 22 58309.0 23 65071.0 24 75313.0 25 89078.0 26 105714.0 27 126506.0 28 153837.0 29 188459.0 30 230099.0 31 285707.0 32 364108.0 33 483252.0 34 689045.0 35 1060603.0 36 1950794.0 37 4029554.0 38 4707802.0 39 734796.0 40 74.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.397146174259724 18.656128231718114 14.14560293912708 24.80112265489508 2 16.138311861014863 20.797851466236665 38.36956690206552 24.694269770682954 3 18.364527669843596 25.088877155767353 29.443685759067357 27.10290941532169 4 13.763705149671052 17.463784062569932 36.440984867692265 32.33152592006675 5 15.223859760749505 36.37937692066453 33.56672405555827 14.830039263027695 6 32.06062519922679 35.02821317858147 18.556567140992247 14.3545944811995 7 28.779684716931865 31.279358017356362 21.897575281624952 18.043381984086817 8 26.721582183830666 33.64098941909542 20.879030140365415 18.7583982567085 9 27.211173574052992 16.256809792499748 19.824822723921958 36.7071939095253 10-11 25.467474205110918 25.505948389735742 27.710809879106947 21.315767526046393 12-13 26.49011737793075 22.7439601170314 27.542354205280546 23.2235682997573 14-15 23.674208160503795 24.15143045967747 24.90369126979062 27.27067011002811 16-17 23.840603758780436 26.996551032120582 25.6079029852472 23.554942223851786 18-19 24.078538390106843 26.284711119198718 26.61193374450967 23.02481674618477 20-21 24.717819765875582 25.67999941469825 26.181867761114795 23.42031305831137 22-23 24.634451203852493 25.568724196612546 25.996547371258522 23.800277228276443 24-25 24.382400874008418 25.746291113952267 26.007090347446983 23.864217664592328 26-27 24.0507079649364 26.088773063480325 25.85411131194798 24.006407659635297 28-29 24.115502207133634 25.99021901974093 25.932690405609588 23.961588367515848 30-31 24.167627933641338 25.695283038985618 25.964725014828144 24.172364012544907 32-33 23.982118878491697 25.821048057025177 26.025828837686802 24.171004226796327 34-35 24.37567221368792 25.750070125505513 25.863544957214373 24.01071270359219 36-37 24.156925974042398 25.55125000836918 25.987398990193306 24.304425027395116 38-39 24.096909134344138 25.582866715569224 26.15046636155669 24.16975778852995 40-41 24.288126560925466 25.2625608932356 26.37738844743537 24.07192409840356 42-43 23.951953821877336 25.30896861247822 26.358300945978126 24.380776619666314 44-45 23.697435766625137 25.494774202652636 26.274447985996147 24.53334204472608 46-47 23.831882987157325 25.392362081779318 26.24573155651205 24.530023374551305 48-49 23.684825341458037 25.45180454964967 26.305600735614494 24.5577693732778 50-51 23.407421366376692 25.634398477306043 26.38641923613007 24.571760920187195 52-53 23.59047446207417 25.477021577785585 26.244968750498842 24.6875352096414 54-55 23.42233012718067 25.32767204254548 26.48044754383281 24.769550286441046 56-57 23.391573933860876 25.522554949145032 26.42508582406336 24.660785292930733 58-59 23.610323843941103 25.235961367617364 26.23980979576956 24.91390499267197 60-61 23.518368697365002 25.09579481669459 26.48501764758412 24.900818838356287 62-63 23.52359963775725 25.082873955735153 26.480289062646513 24.913237343861084 64-65 23.477981012138546 25.124880783815666 26.280688452018858 25.116449752026927 66-67 23.536999467354832 25.333797428394718 26.030246868714357 25.098956235536097 68-69 23.448745761604464 25.055552399271964 26.34129835704126 25.154403482082316 70-71 23.562858247791137 25.059255140932134 26.221413486192336 25.15647312508439 72-73 23.610284737481166 25.0830917436612 26.16630150577035 25.140322013087278 74-75 23.50649288659131 25.23899864222822 26.18927461338715 25.065233857793324 76-77 23.761337282911075 25.046935129822444 26.2585527958829 24.93317479138358 78-79 23.715412949313297 25.040993856663412 26.349626431538205 24.893966762485086 80-81 23.729849483651662 25.126743455747942 26.391585260481143 24.75182180011925 82-83 23.77152691660572 25.047274684818127 26.409074483033045 24.772123915543105 84-85 23.84806458161891 24.903863707863948 26.432132113981783 24.815939596535355 86-87 23.926183891451217 24.860830184202133 26.467166814624974 24.745819109721676 88-89 24.0320675027541 24.615605821417024 26.548937007858154 24.803389667970723 90-91 24.145545610076617 24.586986437231907 26.639635144181895 24.627832808509584 92-93 24.294403842411384 24.469718325707625 26.573361932032814 24.662515899848174 94-95 24.29656972873442 24.415725769315568 26.463562457381702 24.824142044568315 96-97 24.56396845820264 24.25626266723982 26.30564463975551 24.874124234802032 98-99 24.50109141172094 24.10032769487673 26.436015454830347 24.96256543857198 100-101 24.483008017857475 24.007053077240815 26.674807345553674 24.835131559348035 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1210.0 1 752.0 2 386.5 3 468.5 4 411.5 5 407.0 6 648.0 7 1244.0 8 2196.0 9 2743.0 10 2507.5 11 2301.5 12 2490.5 13 2831.5 14 3046.5 15 3214.0 16 3463.5 17 3723.5 18 4011.5 19 4420.0 20 4942.5 21 5660.0 22 6647.0 23 7825.0 24 9437.5 25 11777.0 26 15097.0 27 19421.5 28 24465.0 29 32287.0 30 45081.5 31 63138.5 32 87376.5 33 119363.0 34 161362.0 35 207290.5 36 255410.5 37 301175.0 38 341589.0 39 371673.5 40 399734.5 41 449420.5 42 502645.0 43 551501.5 44 602025.5 45 640339.5 46 670714.0 47 710863.0 48 752566.0 49 778626.0 50 791790.0 51 782632.0 52 754707.5 53 739995.0 54 722363.0 55 672253.5 56 606694.5 57 538786.0 58 473797.0 59 408110.5 60 333982.5 61 262787.0 62 205917.0 63 160572.5 64 124573.0 65 96114.0 66 71438.5 67 53803.0 68 39469.0 69 28126.0 70 20541.5 71 14761.0 72 10959.5 73 8199.0 74 6304.5 75 4820.5 76 3356.5 77 2144.0 78 1304.0 79 847.5 80 633.0 81 455.0 82 348.0 83 285.0 84 228.5 85 205.0 86 172.0 87 153.5 88 147.0 89 124.5 90 95.5 91 77.0 92 67.5 93 66.5 94 63.5 95 53.0 96 47.0 97 43.0 98 37.0 99 21.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029499607332957853 2 0.03556066110851096 3 0.029964412070346898 4 0.025396930850937246 5 0.026940082579068863 6 0.029090579164055497 7 0.029623555262928265 8 0.02778912589936618 9 0.03208392167284092 10-11 0.018049917301941124 12-13 0.02733671595497418 14-15 0.022952057932270883 16-17 0.03752523579854197 18-19 0.042641186608070696 20-21 0.04570579917658911 22-23 0.03581785306319956 24-25 0.014408946859060297 26-27 0.023952937466781954 28-29 0.02915875052553922 30-31 0.02677275287360881 32-33 0.033391572334028764 34-35 0.03594180099316997 36-37 0.032201672206312815 38-39 0.01887726973449362 40-41 0.015806459769476682 42-43 0.015208411007369449 44-45 0.022784728226810828 46-47 0.017836107122742164 48-49 0.02135003093740332 50-51 0.024002516638770118 52-53 0.02944073206622191 54-55 0.029375659402987444 56-57 0.032719154813939284 58-59 0.030593447814946734 60-61 0.03003568213007988 62-63 0.035576154599757256 64-65 0.030512881660465965 66-67 0.02513354149975012 68-69 0.02458197321138179 70-71 0.03446991932477134 72-73 0.016962274216450766 74-75 0.026221184585240476 76-77 0.044398148515401276 78-79 0.027438972997199766 80-81 0.02667669322788174 82-83 0.02657753488390541 84-85 0.02654344920316355 86-87 0.021371721825148144 88-89 0.02455718362538771 90-91 0.021120727266958062 92-93 0.03679394301171655 94-95 0.07172246967737841 96-97 0.08220536585462593 98-99 0.0826763679885135 100-101 0.08356259568780194 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6135808E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.68477706160043 #Duplication Level Percentage of deduplicated Percentage of total 1 74.17746337828511 30.1789356054056 2 12.556192733565686 10.216918042152143 3 4.355960862791909 5.316638897752363 4 2.2073169445883996 3.592167911794883 5 1.3353109276234123 2.7163413699138697 6 0.9036734102605989 2.2059450739769186 7 0.6731021316297983 1.9169507115532458 8 0.5130823878781933 1.6699714052046315 9 0.3913447694268106 1.4329597228517892 >10 2.5221323900838883 19.441512288212355 >50 0.21924407261081888 6.145401763865556 >100 0.13239534555048865 10.218402794233123 >500 0.009544543126879513 2.6271644315108684 >1k 0.0031750537891401145 2.129763629860302 >5k 6.104878884777734E-5 0.1909263517124057 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.197396498520558E-6 0.0 2 0.0 0.0 0.0 1.8592189495561672E-5 0.0 3 0.0 0.0 0.0 1.8592189495561672E-5 0.0 4 0.0 0.0 0.0 4.957917198816446E-5 0.0 5 0.0 0.0 0.0 8.056615448076725E-5 0.0 6 0.0 0.0 0.0 8.056615448076725E-5 6.197396498520558E-6 7 6.197396498520558E-6 0.0 0.0 9.296094747780836E-5 6.197396498520558E-6 8 6.197396498520558E-6 0.0 0.0 1.3014532646893172E-4 6.197396498520558E-6 9 6.197396498520558E-6 0.0 0.0 2.169088774482195E-4 1.2394792997041115E-5 10-11 6.197396498520558E-6 6.197396498520558E-6 0.0 3.501529021664115E-4 1.2394792997041115E-5 12-13 1.2394792997041115E-5 6.197396498520558E-6 0.0 4.771995303860829E-4 3.098698249260279E-5 14-15 3.098698249260279E-5 6.197396498520558E-6 0.0 6.383318393476175E-4 3.7184378991123344E-5 16-17 3.7184378991123344E-5 6.197396498520558E-6 0.0 8.273524325524944E-4 4.3381775489643906E-5 18-19 4.0283077240383625E-5 6.197396498520558E-6 0.0 0.001078346990742577 6.197396498520558E-5 20-21 4.3381775489643906E-5 1.2394792997041115E-5 0.0 0.0014439933841552898 6.197396498520558E-5 22-23 4.957917198816446E-5 1.2394792997041115E-5 0.0 0.002144299188488113 7.127005973298641E-5 24-25 8.056615448076725E-5 1.2394792997041115E-5 0.0 0.0038423858290827454 7.746745623150697E-5 26-27 8.056615448076725E-5 1.2394792997041115E-5 0.0 0.007412086212230587 8.056615448076725E-5 28-29 8.366485273002753E-5 1.2394792997041115E-5 0.0 0.026388514290700535 8.366485273002753E-5 30-31 8.676355097928781E-5 1.2394792997041115E-5 0.0 0.08821684045819088 8.986224922854808E-5 32-33 8.676355097928781E-5 1.5493491246301394E-5 0.0 0.22668217172638644 9.915834397632892E-5 34-35 1.022570422255892E-4 2.7888284243342508E-5 0.0 0.40254879086315354 1.0535574047484948E-4 36-37 1.3634272296745225E-4 3.7184378991123344E-5 0.0 0.6790580304376452 1.0535574047484948E-4 38-39 1.4873751596449338E-4 5.267787023742474E-5 0.0 1.1091852357192153 1.0535574047484948E-4 40-41 1.4873751596449338E-4 6.197396498520558E-5 0.0 1.5781267352710195 1.0535574047484948E-4 42-43 1.7042840370931533E-4 6.197396498520558E-5 0.0 2.0465166665344556 1.1465183522263033E-4 44-45 2.1690887744821953E-4 6.507266323446586E-5 0.0 2.5680616675657024 1.3324402471819198E-4 46-47 2.478958599408223E-4 8.056615448076725E-5 0.0 3.1421481961114064 1.580336107122742E-4 48-49 2.5409325643934285E-4 8.056615448076725E-5 0.0 3.7238110418765515 2.0761278270043867E-4 50-51 2.5719195468860317E-4 8.056615448076725E-5 0.0 4.300677102751842 2.169088774482195E-4 52-53 2.6029065293786343E-4 8.056615448076725E-5 0.0 4.851879744726759 2.2310627394674006E-4 54-55 2.6648804943638397E-4 8.056615448076725E-5 0.0 5.423574078224034 2.355010669437812E-4 56-57 2.912776354304662E-4 8.056615448076725E-5 0.0 6.046211010939149 2.355010669437812E-4 58-59 2.943763336797265E-4 8.056615448076725E-5 0.0 6.683746484836706 2.355010669437812E-4 60-61 2.9747503192898675E-4 8.056615448076725E-5 0.0 7.319723313514886 2.355010669437812E-4 62-63 3.22264617923069E-4 8.056615448076725E-5 0.0 7.934604204512101 2.355010669437812E-4 64-65 3.22264617923069E-4 8.056615448076725E-5 0.0 8.548648446982018 2.355010669437812E-4 66-67 3.594489969141923E-4 9.296094747780836E-5 0.0 9.203074925036292 2.3859976519304145E-4 68-69 3.656463934127129E-4 9.296094747780836E-5 0.0 9.88659817965112 2.4479716169156204E-4 70-71 3.718437899112335E-4 9.605964572706864E-5 0.0 10.572935052276279 2.509945581900826E-4 72-73 3.8733728115753483E-4 1.0535574047484948E-4 0.0 11.235563784596348 2.6648804943638397E-4 74-75 4.090281689023568E-4 1.0535574047484948E-4 0.0 11.893804760195462 2.726854459349045E-4 76-77 4.090281689023568E-4 1.0535574047484948E-4 0.0 12.57502258331284 2.788828424334251E-4 78-79 4.090281689023568E-4 1.0535574047484948E-4 0.0 13.280605470764154 2.8508023893194563E-4 80-81 4.462125478934801E-4 1.1155313697337003E-4 0.0 13.994492869523484 2.912776354304662E-4 82-83 4.524099443920007E-4 1.1155313697337003E-4 0.0 14.704448020204504 3.1916591967380873E-4 84-85 4.710021338875624E-4 1.1155313697337003E-4 0.0 15.403319127247919 3.22264617923069E-4 86-87 4.895943233831241E-4 1.1465183522263031E-4 0.0 16.101985720206883 3.346594109201101E-4 88-89 4.988904181309049E-4 1.177505334718906E-4 0.0 16.82644587739269 3.501529021664115E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14710 0.0 45.242374 1 GTATCAA 24875 0.0 43.941353 1 ATCAACG 29900 0.0 36.37763 3 TCAACGC 30275 0.0 35.863953 4 CAACGCA 31035 0.0 34.939457 5 TATCAAC 31750 0.0 34.00525 2 AACGCAG 32440 0.0 33.575012 6 ACGCAGA 38795 0.0 27.97706 7 CGCAGAG 39750 0.0 27.16126 8 GTACATG 54570 0.0 25.270452 1 TACATGG 56065 0.0 24.298397 2 ACATGGG 57470 0.0 23.413994 3 CATGGGG 44760 0.0 21.902096 4 GCAGAGT 48710 0.0 21.823353 9 AGAGTAC 43590 0.0 18.622417 10-11 CAGAGTA 44980 0.0 18.532692 10-11 ATGGGGA 27535 0.0 18.336237 5 CGTATCA 4120 0.0 16.901438 94-95 TCGCCGT 10895 0.0 16.883677 94-95 TAGGACG 9255 0.0 16.217232 4 >>END_MODULE