FastQCFastQC Report
Fri 27 May 2016
SRR940201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940201_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16135808
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158750.046.572041
GTATCAA265900.044.0124471
ATCAACG321900.035.4884953
TCAACGC325250.035.1376884
CAACGCA335450.034.068735
TATCAAC340300.033.8507922
AACGCAG350200.032.755466
ACGCAGA413650.027.6046877
CGCAGAG425950.026.6068338
GTACATG558500.024.3572141
TACATGG570150.023.520192
ACATGGG588750.022.299783
GCAGAGT516750.021.7201049
CATGGGG451350.020.3744894
AGAGTAC463250.018.73783110-11
CAGAGTA476950.018.70251710-11
TAGGACG90000.017.2056054
GAGTACT163750.016.82517812-13
ATGGGGA277400.016.780615
GCTTATA67350.016.1557561