FastQCFastQC Report
Fri 27 May 2016
SRR940200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16347026
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145850.045.069631
GTATCAA253250.045.0107351
ATCAACG309700.035.9036373
TCAACGC312400.035.5930024
TATCAAC323700.034.6880872
CAACGCA320950.034.645565
AACGCAG332350.033.5422066
ACGCAGA399800.027.7411827
CGCAGAG411250.026.8648668
GTACATG559400.025.3780231
TACATGG575250.024.291912
ACATGGG589200.023.209043
GCAGAGT499650.021.8690819
CATGGGG457700.021.585234
AGAGTAC442950.018.7377810-11
CAGAGTA457150.018.33761610-11
ATGGGGA281650.018.2136755
TCGCCGT96650.016.89448294-95
GTCCTAC88950.016.766331
TAGGACG92100.016.7093544
Produced by FastQC (version 0.11.5)