##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940200_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16347026 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39635129961866 34.0 31.0 34.0 31.0 34.0 2 32.40517425004401 34.0 31.0 34.0 31.0 34.0 3 32.50980747201356 34.0 31.0 34.0 31.0 34.0 4 35.891469677726086 37.0 37.0 37.0 35.0 37.0 5 35.90176457785043 37.0 37.0 37.0 35.0 37.0 6 35.97457800580974 37.0 37.0 37.0 35.0 37.0 7 35.937159639924715 37.0 37.0 37.0 35.0 37.0 8 35.927913493255595 37.0 37.0 37.0 35.0 37.0 9 37.69552755345223 39.0 39.0 39.0 35.0 39.0 10-11 37.62815719507634 39.0 38.5 39.0 35.0 39.0 12-13 37.57079254049024 39.0 38.0 39.0 35.0 39.0 14-15 39.02761453367725 41.0 39.0 41.0 36.0 41.0 16-17 38.94615867742549 41.0 39.0 41.0 36.0 41.0 18-19 38.90421138377097 41.0 39.0 41.0 36.0 41.0 20-21 38.83907907774784 41.0 39.0 41.0 36.0 41.0 22-23 38.73144090552006 40.5 39.0 41.0 35.0 41.0 24-25 38.617740804963546 40.0 39.0 41.0 35.0 41.0 26-27 38.46474404579769 40.0 38.0 41.0 34.5 41.0 28-29 38.313677331889 40.0 38.0 41.0 34.0 41.0 30-31 38.283629633916284 40.0 38.0 41.0 34.0 41.0 32-33 38.30999244143858 40.0 38.0 41.0 34.0 41.0 34-35 38.346499143024545 40.0 38.5 41.0 34.5 41.0 36-37 38.489461905792524 41.0 39.0 41.0 35.0 41.0 38-39 38.42206395830043 41.0 39.0 41.0 35.0 41.0 40-41 38.310993388032784 41.0 38.0 41.0 34.0 41.0 42-43 38.20874466707278 40.0 38.0 41.0 34.0 41.0 44-45 38.07127164904491 40.0 38.0 41.0 33.5 41.0 46-47 37.916114894538005 40.0 38.0 41.0 33.0 41.0 48-49 37.7483408908752 40.0 38.0 41.0 33.0 41.0 50-51 36.81722026991332 39.0 36.5 40.5 31.5 40.5 52-53 36.903420505968484 39.5 37.0 40.5 32.0 41.0 54-55 37.17316862406654 40.0 37.0 41.0 32.0 41.0 56-57 37.120827054413446 40.0 36.5 41.0 32.0 41.0 58-59 36.904992229167554 39.5 36.0 41.0 32.0 41.0 60-61 36.61807490243179 39.0 35.5 41.0 31.5 41.0 62-63 36.28664700233547 39.0 35.0 41.0 31.0 41.0 64-65 35.91359648537905 38.0 35.0 40.5 31.0 41.0 66-67 35.52705002732607 37.0 35.0 40.0 30.0 41.0 68-69 35.11389093037474 37.0 35.0 39.5 30.0 41.0 70-71 34.68328694772982 36.0 35.0 39.0 29.5 41.0 72-73 34.24202778535985 36.0 34.0 39.0 29.0 40.0 74-75 33.78669820430946 35.0 34.0 37.5 29.0 39.5 76-77 33.31814897095043 35.0 34.0 37.0 28.0 39.0 78-79 32.871345466753404 35.0 34.0 36.5 27.0 39.0 80-81 32.425499690280056 35.0 33.0 36.0 26.0 37.0 82-83 32.01977215916828 35.0 33.0 35.5 25.5 37.0 84-85 31.630386805526584 35.0 33.0 35.0 24.5 36.5 86-87 31.280460953570394 35.0 32.5 35.0 23.5 36.0 88-89 30.90502498741973 35.0 32.0 35.0 20.0 36.0 90-91 30.551383780756204 34.5 32.0 35.0 18.5 35.5 92-93 30.153890377368946 34.0 31.0 35.0 9.5 35.0 94-95 29.71856654537651 34.0 31.0 35.0 2.0 35.0 96-97 29.25981961489509 34.0 30.5 35.0 2.0 35.0 98-99 28.713588392163807 34.0 30.0 35.0 2.0 35.0 100-101 26.650001535447487 32.0 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13952023129142077 1101 2 0.1680597186908983 1101 3 0.17884901079108317 1101 4 0.16121155481286564 1101 5 0.1382333915090186 1101 6 0.09347449653981954 1101 7 0.10263578453827904 1101 8 0.13164167237743385 1101 9 0.09863591043497166 1101 10-11 0.12314200345235093 1101 12-13 0.16601865010869687 1101 14-15 0.18310962650595997 1101 16-17 0.20636345275726597 1101 18-19 0.20324625364888504 1101 20-21 0.1401866004488923 1101 22-23 0.18849120891008653 1101 24-25 0.16372016375571974 1101 26-27 0.20063289929087347 1101 28-29 0.21024675379621982 1101 30-31 0.21354671564309058 1101 32-33 0.20806286183945844 1101 34-35 0.13222335687314768 1101 36-37 0.18038537974420876 1101 38-39 0.26653187757114694 1101 40-41 0.2246132826433893 1101 42-43 0.22595162068079588 1101 44-45 0.23282539496592847 1101 46-47 0.24494454145771272 1101 48-49 0.23713698801476113 1101 50-51 0.16730052058473177 1101 52-53 0.2181561790725297 1101 54-55 0.3764139758383678 1101 56-57 0.28720960360559644 1101 58-59 0.2879310596008011 1101 60-61 0.2515640661676528 1101 62-63 0.2510807050125692 1101 64-65 0.3193622664397111 1101 66-67 0.2938211147319194 1101 68-69 0.2860033250079326 1101 70-71 0.264047457003457 1101 72-73 0.17804325454664394 1101 74-75 0.16105292490708223 1101 76-77 0.156600199061657 1101 78-79 0.12846974006904333 1101 80-81 0.06480565698314678 1101 82-83 -0.10610828079384049 1101 84-85 0.04811568645177999 1101 86-87 0.05779276374191866 1101 88-89 0.06385027790436837 1101 90-91 -0.012162877516022519 1101 92-93 0.006794227798437191 1101 94-95 -0.025454209165694408 1101 96-97 -0.04007350877149918 1101 98-99 -0.07870527070565458 1101 100-101 0.22374442475674172 1102 1 0.147565311759692 1102 2 0.16969860887841293 1102 3 0.141910098318057 1102 4 0.15200602129254293 1102 5 0.1390157527339042 1102 6 0.1413468299151006 1102 7 0.14469563771447014 1102 8 0.2011286772488745 1102 9 0.19988642103873389 1102 10-11 0.23816610113534864 1102 12-13 0.24658598773007157 1102 14-15 0.2913924065965219 1102 16-17 0.2892304157010699 1102 18-19 0.2371806977468438 1102 20-21 0.28805258149858304 1102 22-23 0.3223479270188676 1102 24-25 0.34499135729928554 1102 26-27 0.26734957143087 1102 28-29 0.2934186062781947 1102 30-31 0.29919153240967233 1102 32-33 0.2121999857057304 1102 34-35 0.2488276446405635 1102 36-37 0.2782233112345551 1102 38-39 0.3071125405127404 1102 40-41 0.2823349218209259 1102 42-43 0.23681521427386087 1102 44-45 0.23144571549917003 1102 46-47 0.22544558956689542 1102 48-49 0.22889147670107235 1102 50-51 0.20894222603721602 1102 52-53 0.2175520188261686 1102 54-55 0.3269056506673067 1102 56-57 0.2595252682229017 1102 58-59 0.25595054266778305 1102 60-61 0.204262315057953 1102 62-63 0.2065098014161748 1102 64-65 0.24412087602134136 1102 66-67 0.22845273065571803 1102 68-69 0.26383071207959574 1102 70-71 0.2767040648612564 1102 72-73 0.24642473671897136 1102 74-75 0.19007186229915618 1102 76-77 0.13418672916630214 1102 78-79 0.17664776684132022 1102 80-81 0.1639919788094062 1102 82-83 0.15244304050706603 1102 84-85 0.07050008652117867 1102 86-87 0.10839820688969226 1102 88-89 0.08246550463174884 1102 90-91 -0.007497921128202023 1102 92-93 0.03722753126776723 1102 94-95 -0.07020697099142836 1102 96-97 -0.07345700071360994 1102 98-99 -0.058905542661118915 1102 100-101 0.2042019438083571 1103 1 0.051303023809389 1103 2 0.10358556810483321 1103 3 0.03916119432268772 1103 4 0.050441619201819776 1103 5 0.05040469677040704 1103 6 0.05507658785642633 1103 7 0.011459694062281756 1103 8 0.04273742151761439 1103 9 0.03142578204007407 1103 10-11 0.013403445282868631 1103 12-13 0.03181649682421295 1103 14-15 -0.010876550929381779 1103 16-17 -0.032688358440388754 1103 18-19 -0.058007896095055855 1103 20-21 0.012467851841329036 1103 22-23 -0.08537430090034803 1103 24-25 -0.06001123485516047 1103 26-27 -0.07979069522679794 1103 28-29 -0.021818693897969865 1103 30-31 -0.01845862034153356 1103 32-33 -0.06137031449278396 1103 34-35 -0.08657054614330661 1103 36-37 -0.06520539208997889 1103 38-39 -0.10500750824183314 1103 40-41 -0.03533842858418268 1103 42-43 -0.05814028990542397 1103 44-45 -0.07135255107912286 1103 46-47 -0.11388080252277888 1103 48-49 -0.06573230676111308 1103 50-51 -0.11557752252117837 1103 52-53 -0.08775978759576475 1103 54-55 -0.034498960732086914 1103 56-57 -0.10485352243004087 1103 58-59 -0.022857858167334655 1103 60-61 0.0044122787066598335 1103 62-63 0.04676230640592394 1103 64-65 0.023963327156991454 1103 66-67 0.058116110652626674 1103 68-69 -0.00353648643304183 1103 70-71 -0.04527122551106544 1103 72-73 -0.0740915194863021 1103 74-75 -0.0169258550224356 1103 76-77 -0.04185074838838432 1103 78-79 -0.02495671244394515 1103 80-81 -0.11884926485800662 1103 82-83 -0.10892010377613559 1103 84-85 -0.1236695614944665 1103 86-87 -0.23175523998569858 1103 88-89 -0.2427345917904553 1103 90-91 -0.2838896179411101 1103 92-93 -0.22602623465969174 1103 94-95 -0.21063279149984027 1103 96-97 -0.40723279902855225 1103 98-99 -0.49087528785898016 1103 100-101 -0.2870716654393064 1104 1 0.10948033698456072 1104 2 0.106800809070009 1104 3 0.14493179619883279 1104 4 0.1250556035529229 1104 5 0.148004864818283 1104 6 0.16427307726237217 1104 7 0.11278048840234334 1104 8 0.11360513661395544 1104 9 0.15117099322639405 1104 10-11 0.19191106021695958 1104 12-13 0.16725141621420647 1104 14-15 0.15315453406271473 1104 16-17 0.13973384439854186 1104 18-19 0.25222122126385926 1104 20-21 0.25149258620245263 1104 22-23 0.23892789635564782 1104 24-25 0.24146778618925424 1104 26-27 0.268638187835883 1104 28-29 0.21549801859783457 1104 30-31 0.2586481318562335 1104 32-33 0.19011188766716458 1104 34-35 0.23318557030053455 1104 36-37 0.18916232402831668 1104 38-39 0.11748450216531836 1104 40-41 0.10205129406998026 1104 42-43 0.20663221646400132 1104 44-45 0.2046536053180006 1104 46-47 0.13254258420269593 1104 48-49 0.15984002090598182 1104 50-51 0.06361191067927052 1104 52-53 0.06891632281632099 1104 54-55 0.28678851455281773 1104 56-57 0.14264630501586595 1104 58-59 0.1450307114527618 1104 60-61 0.17598289321523453 1104 62-63 0.11910673709594022 1104 64-65 0.1954776437242316 1104 66-67 0.18153058551100543 1104 68-69 0.20234099524979143 1104 70-71 0.1754896510021311 1104 72-73 0.13261401092380964 1104 74-75 0.12195336252445088 1104 76-77 0.11619797761980521 1104 78-79 0.046346336712915104 1104 80-81 0.09221443218819303 1104 82-83 0.06643052002104355 1104 84-85 0.0053970218978278695 1104 86-87 0.02995525621268058 1104 88-89 0.05751874396035461 1104 90-91 0.059157885990281045 1104 92-93 0.05716581075603244 1104 94-95 0.011156378550200685 1104 96-97 0.009466307372683502 1104 98-99 0.07721707793118426 1104 100-101 0.25430767615210925 1105 1 -0.191762590393175 1105 2 -0.04627516766424833 1105 3 -0.07313998440178437 1105 4 0.007432032446104131 1105 5 0.03514169013581636 1105 6 0.07889340526950406 1105 7 0.07192540097780409 1105 8 0.029770547898344546 1105 9 0.052728117216759074 1105 10-11 -0.010464223011908302 1105 12-13 0.014787698239850045 1105 14-15 0.03918478905672629 1105 16-17 0.0028798194853720815 1105 18-19 0.053777759304921346 1105 20-21 -0.03876779651091056 1105 22-23 0.06068965751943978 1105 24-25 0.05927389910700498 1105 26-27 0.16577906947106413 1105 28-29 0.1643795105439665 1105 30-31 0.14945627342891754 1105 32-33 0.16280054094053753 1105 34-35 0.08197064784143038 1105 36-37 0.10163351291259914 1105 38-39 0.14085851578976616 1105 40-41 0.060328734831067266 1105 42-43 0.0592370068998207 1105 44-45 0.009350170102102595 1105 46-47 -0.049979193942874645 1105 48-49 0.02758878370729434 1105 50-51 -0.06756106517672578 1105 52-53 0.008042219363851189 1105 54-55 0.29443970255129415 1105 56-57 0.1915281536431408 1105 58-59 0.15466585273923528 1105 60-61 0.16221468582740783 1105 62-63 0.16877152899546388 1105 64-65 0.18067080080160736 1105 66-67 0.24019089621763356 1105 68-69 0.23511221678487004 1105 70-71 0.2642510998650849 1105 72-73 0.2373422887572545 1105 74-75 0.12820498675786496 1105 76-77 0.11292127886070347 1105 78-79 0.18832050987192872 1105 80-81 0.3226052386459557 1105 82-83 0.33903731215588095 1105 84-85 0.3244822764157078 1105 86-87 0.2585764694805768 1105 88-89 0.2941079844368595 1105 90-91 0.18971463411615375 1105 92-93 0.1417680099654426 1105 94-95 0.20160609580166522 1105 96-97 0.0639603569542011 1105 98-99 -0.07614544642649079 1105 100-101 0.13738947486957542 1106 1 -0.17585294188085498 1106 2 -0.07233801220355929 1106 3 -0.05810574535518498 1106 4 0.06797407741410666 1106 5 0.07166268688403221 1106 6 0.06567050685136167 1106 7 0.07744934261166492 1106 8 0.048741730002028305 1106 9 -0.04908216361547346 1106 10-11 -0.03449565256905629 1106 12-13 0.08735109116261697 1106 14-15 0.0834632887401412 1106 16-17 -0.06710286591771819 1106 18-19 0.16495514841372483 1106 20-21 0.12599541945920834 1106 22-23 0.18088927491744755 1106 24-25 0.18957735720656643 1106 26-27 0.18957861953682453 1106 28-29 0.18088301709940424 1106 30-31 0.14042584671599911 1106 32-33 0.22024898996318854 1106 34-35 -0.001214434366026751 1106 36-37 0.12244804985985525 1106 38-39 0.11199664644666285 1106 40-41 0.19553227303078557 1106 42-43 0.27021988451780743 1106 44-45 0.20446589474099852 1106 46-47 0.2163859262566845 1106 48-49 0.19385836375752064 1106 50-51 0.12953080914408588 1106 52-53 0.21429080907944353 1106 54-55 0.3606291265313857 1106 56-57 0.3016076445818996 1106 58-59 0.2971755787951622 1106 60-61 0.35126515253914903 1106 62-63 0.3481492480467523 1106 64-65 0.19988526020524944 1106 66-67 0.29596832948386975 1106 68-69 0.32263302093307544 1106 70-71 0.3091573844019635 1106 72-73 0.2938253474663526 1106 74-75 0.3763973565271286 1106 76-77 0.22959630355003213 1106 78-79 0.1640828403382315 1106 80-81 0.37955728866513994 1106 82-83 0.5074697615436108 1106 84-85 0.37586203668935525 1106 86-87 0.39086035051453294 1106 88-89 0.39105730727668586 1106 90-91 0.1854615055547093 1106 92-93 0.23416677994712032 1106 94-95 0.253983192098616 1106 96-97 0.24750538773331954 1106 98-99 0.30233271446873644 1106 100-101 0.5673623887440016 1107 1 0.010952132544773008 1107 2 0.05763281995596259 1107 3 0.053007166191555655 1107 4 0.0801687792241168 1107 5 0.033798589219912856 1107 6 0.034520062737541934 1107 7 0.0970502580267194 1107 8 0.032808825356575255 1107 9 -0.026200383311682174 1107 10-11 0.026142733339007407 1107 12-13 0.09411894114845154 1107 14-15 0.11025060201666292 1107 16-17 0.10298596482267186 1107 18-19 0.13440030252888846 1107 20-21 0.15205936351257776 1107 22-23 0.1644549424220969 1107 24-25 0.18910272582048293 1107 26-27 0.15233168972404343 1107 28-29 0.1817861213070202 1107 30-31 0.17603640663626408 1107 32-33 0.1781540899198717 1107 34-35 0.21186056190059332 1107 36-37 0.17697585041486263 1107 38-39 0.1672531516621092 1107 40-41 0.17162661750500519 1107 42-43 0.1259981759170259 1107 44-45 0.20952495744428035 1107 46-47 0.18782307665335196 1107 48-49 0.16040232711630864 1107 50-51 0.14812256496098541 1107 52-53 0.21830173162572208 1107 54-55 0.34447736570212584 1107 56-57 0.3028986673029479 1107 58-59 0.2910110373415833 1107 60-61 0.32831680477755754 1107 62-63 0.28254737443914735 1107 64-65 0.31180790313670315 1107 66-67 0.21845938344917926 1107 68-69 0.2808992286973009 1107 70-71 0.25598926583054293 1107 72-73 0.3208331512047522 1107 74-75 0.28448419141163583 1107 76-77 0.28016153162258206 1107 78-79 0.2911631134502386 1107 80-81 0.37318258047368147 1107 82-83 0.4267009683621268 1107 84-85 0.3734847050507426 1107 86-87 0.33734951421469717 1107 88-89 0.37876301597810524 1107 90-91 0.39478444322732287 1107 92-93 0.4021012956988095 1107 94-95 0.3824553716687902 1107 96-97 0.45846201422786237 1107 98-99 0.4167669312757134 1107 100-101 0.5780687812715186 1108 1 0.24627191665917536 1108 2 0.25689224550491474 1108 3 0.2508928391799117 1108 4 0.1933803261400513 1108 5 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-0.9288508179982813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 99019.0 3 25991.0 4 20766.0 5 17177.0 6 15338.0 7 15052.0 8 16030.0 9 18422.0 10 20945.0 11 24274.0 12 27069.0 13 29819.0 14 33072.0 15 37235.0 16 44661.0 17 52860.0 18 50922.0 19 45637.0 20 47756.0 21 52503.0 22 56014.0 23 63652.0 24 73862.0 25 87347.0 26 103547.0 27 126269.0 28 153999.0 29 188183.0 30 231846.0 31 289507.0 32 370422.0 33 491335.0 34 697539.0 35 1071054.0 36 1964451.0 37 4071234.0 38 4840680.0 39 771436.0 40 101.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.41511387790633 18.63099316865338 14.154685790300963 24.79920716313933 2 15.447872827046435 20.73639394120194 38.76865837184718 25.047074859904445 3 18.389692938309253 25.063694053161694 29.441980252059448 27.1046327564696 4 13.766286260287444 17.45240106847951 36.466073034304614 32.315239636928425 5 15.239953878055424 36.3776954217281 33.55654818823389 14.825802511982594 6 32.02261126659561 35.05642720694504 18.56152841735819 14.359433109101166 7 28.80097570688359 31.28054858791553 21.89438127183211 18.024094433368766 8 26.743209159396063 33.65064986518745 20.873847783778015 18.732293191638476 9 27.22670233432976 16.232223507945832 19.830449286872117 36.710624870852286 10-11 25.4579702187531 25.49568178681485 27.73456979303398 21.31177820139807 12-13 26.478389310893398 22.739714426606657 27.552647290064492 23.229248972435453 14-15 23.65914136679289 24.157225364903205 24.9026394440596 27.28099382424431 16-17 23.85162862033012 26.98514619140779 25.618574136455187 23.544651051806905 18-19 24.073553308513933 26.273486674095516 26.630701135086227 23.022258882304328 20-21 24.70605362962866 25.675419024789164 26.179771633207217 23.43875571237496 22-23 24.636313875336185 25.574966306432213 25.992792862924492 23.79592695530711 24-25 24.410530087053417 25.720866709490203 26.00598373127744 23.86261947217894 26-27 24.03012580002726 26.084803998579805 25.86929016611441 24.015780035278528 28-29 24.128438287081984 25.97086810556225 25.926165925765744 23.974527681590025 30-31 24.19197896635917 25.67945199846634 25.952386226362517 24.176182808811973 32-33 23.987294663052282 25.79136340863204 26.050117352008968 24.17122457630671 34-35 24.35627751570126 25.7623507530861 25.873823413414033 24.00754831779861 36-37 24.192833529401867 25.523362241331043 25.97507966399616 24.308724565270936 38-39 24.098323640072767 25.56172724637134 26.154895629132156 24.185053484423733 40-41 24.27941533542993 25.272137085009817 26.37801583096635 24.070431748593904 42-43 23.939438493545055 25.31111590805242 26.364598254941278 24.384847343461242 44-45 23.709866341906135 25.481249322553 26.277096388512653 24.53178794702821 46-47 23.822502109807775 25.373959929085622 26.262011036924747 24.54152692418186 48-49 23.658564523107476 25.44418363896036 26.329197790926358 24.56805404700581 50-51 23.4075723558199 25.617421747540618 26.38968420015718 24.5853216964823 52-53 23.575959639633112 25.50792051212028 26.25405176555189 24.66206808269472 54-55 23.46056162428239 25.318619376610457 26.46884006821689 24.751978930890264 56-57 23.38932207207304 25.509698910747552 26.442244239769785 24.658734777409627 58-59 23.602699444319335 25.207789411060727 26.25040081435258 24.939110330267354 60-61 23.543174694952665 25.07716852440761 26.47767422153083 24.901982559108895 62-63 23.506896876754446 25.079681717958675 26.496564785693415 24.91685661959346 64-65 23.48592282119881 25.1147355762443 26.275740265766622 25.12360133679027 66-67 23.525465189042297 25.326799040199855 26.023515317751393 25.12422045300646 68-69 23.443197130574728 25.042068352908835 26.32367277379153 25.19106174272491 70-71 23.584389545624834 25.040012229171644 26.22020317412605 25.155395051077477 72-73 23.626797322772777 25.073197448674133 26.15280034909235 25.147204879460745 74-75 23.518538327213125 25.215209794745842 26.175874595569464 25.090377282471565 76-77 23.72509601924617 25.04634079746288 26.272277005194006 24.95628617809695 78-79 23.729029382155257 25.02254605776325 26.341982630425925 24.906441929655564 80-81 23.744908915446327 25.08797195862078 26.400355718155893 24.766763407776995 82-83 23.77795750680449 25.006093845962013 26.41442692334566 24.80152172388784 84-85 23.862745446000655 24.890256081382088 26.421714291309094 24.825284181308163 86-87 23.931308956852696 24.837524868861042 26.470539286865446 24.760626887420816 88-89 24.034680905544196 24.61598914479261 26.522571247854117 24.826758701809084 90-91 24.1584558863459 24.59887423421417 26.592330493041484 24.65033938639845 92-93 24.319249119356044 24.448433794169933 26.549684432665295 24.682632653808724 94-95 24.31917774841962 24.399620194114846 26.44824563229354 24.832956425171997 96-97 24.588035337302053 24.24252443939897 26.28888677640267 24.880553446896307 98-99 24.577753222723356 24.049922942362112 26.398985624580085 24.973338210334447 100-101 24.517402794332067 23.955904429475765 26.68045518012233 24.84623759606984 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 803.0 1 608.0 2 501.5 3 543.0 4 419.0 5 438.0 6 679.5 7 1271.5 8 2164.0 9 2659.0 10 2487.5 11 2307.0 12 2469.5 13 2691.5 14 2947.0 15 3186.0 16 3398.0 17 3636.0 18 3916.5 19 4331.0 20 4850.5 21 5479.5 22 6344.0 23 7632.5 24 9332.5 25 11688.5 26 14919.0 27 19151.5 28 24439.5 29 32249.0 30 45120.0 31 64019.5 32 88124.5 33 120204.5 34 163230.5 35 209676.5 36 258568.5 37 304904.5 38 345374.0 39 375991.0 40 405358.0 41 455863.0 42 509641.0 43 559354.5 44 609666.0 45 647304.0 46 679570.5 47 720883.5 48 762835.0 49 790740.5 50 803505.5 51 793674.0 52 765984.5 53 750344.5 54 733107.5 55 681786.5 56 614414.5 57 547174.5 58 480870.5 59 414482.5 60 338726.5 61 264944.5 62 208125.5 63 162116.5 64 125582.0 65 97380.5 66 72071.5 67 54143.0 68 39992.0 69 28352.5 70 20605.5 71 14835.5 72 11067.5 73 8276.5 74 6302.5 75 4760.0 76 3284.0 77 2086.0 78 1269.0 79 803.0 80 591.0 81 470.5 82 353.0 83 294.5 84 242.0 85 212.5 86 171.5 87 161.0 88 155.5 89 124.0 90 106.0 91 94.5 92 79.5 93 73.5 94 70.5 95 51.5 96 36.0 97 33.5 98 33.0 99 21.5 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022566795941965225 2 0.03365138099125798 3 0.024811852626893727 4 0.023258053177379175 5 0.04199540638156445 6 0.0235272152867439 7 0.02699573610514842 8 0.040129623578013515 9 0.026335065473071368 10-11 0.027898040903586992 12-13 0.026041434081037124 14-15 0.0349054317280709 16-17 0.04225233384959442 18-19 0.04356755779308114 20-21 0.03717801635600261 22-23 0.043506384586407335 24-25 0.01963965800262384 26-27 0.025836503838679893 28-29 0.027053850651488534 30-31 0.027185373045837208 32-33 0.03682015309696088 34-35 0.0409646378491109 36-37 0.030286854624198924 38-39 0.015871388471517693 40-41 0.01785951768841623 42-43 0.020052577147671998 44-45 0.021389211713494553 46-47 0.015461527986803227 48-49 0.024879143154234905 50-51 0.018174559702786305 52-53 0.02727713285584791 54-55 0.02360062313475246 56-57 0.023239701215377037 58-59 0.02285736867366578 60-61 0.019887409489652737 62-63 0.022771726184322456 64-65 0.019884350829319046 66-67 0.018342786021139257 68-69 0.022533150678294634 70-71 0.035309174892117996 72-73 0.024912788417905497 74-75 0.03382878329061201 76-77 0.04246949873328641 78-79 0.02672351533545001 80-81 0.022939952502675408 82-83 0.016321011540570134 84-85 0.03270319628781406 86-87 0.020150454278350077 88-89 0.0306324832419059 90-91 0.02403189424180276 92-93 0.0419189398732222 94-95 0.07564678737282243 96-97 0.09517633360343343 98-99 0.07249636722912167 100-101 0.08038465222970832 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6347026E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.10460608502437 #Duplication Level Percentage of deduplicated Percentage of total 1 73.91294311064586 29.64249468037268 2 12.622598143181143 10.124486526036797 3 4.270267922258034 5.137722386990218 4 2.327944287451256 3.7344515454446148 5 1.4336216609237897 2.874741599315348 6 0.9421522678712093 2.2670787345052323 7 0.6582970948952876 1.8480521974383994 8 0.4916957587668904 1.5775411775218648 9 0.4034508392041894 1.4562213282860872 >10 2.5636807006799023 19.475780691313187 >50 0.22254195537315283 6.1753288235814106 >100 0.1370401242807295 10.43196870820137 >500 0.010310002977261751 2.796595057681322 >1k 0.003394971881687434 2.2653120137026512 >5k 6.115960967851316E-5 0.19222452960886205 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2234641334760218E-5 2 0.0 0.0 0.0 0.0 1.2234641334760218E-5 3 0.0 0.0 0.0 1.2234641334760218E-5 1.2234641334760218E-5 4 0.0 0.0 0.0 3.058660333690055E-5 1.2234641334760218E-5 5 0.0 0.0 0.0 4.282124467166077E-5 1.835196200214033E-5 6 1.2234641334760218E-5 0.0 0.0 4.282124467166077E-5 3.058660333690055E-5 7 1.2234641334760218E-5 0.0 0.0 4.893856533904087E-5 3.058660333690055E-5 8 1.2234641334760218E-5 0.0 0.0 7.952516867594143E-5 3.058660333690055E-5 9 1.2234641334760218E-5 0.0 0.0 1.835196200214033E-4 3.058660333690055E-5 10-11 1.2234641334760218E-5 6.117320667380109E-6 0.0 3.3951129703959607E-4 3.058660333690055E-5 12-13 1.5293301668450275E-5 6.117320667380109E-6 0.0 4.587990500535082E-4 5.811454634011104E-5 14-15 3.058660333690055E-5 6.117320667380109E-6 0.0 6.209080477390811E-4 6.11732066738011E-5 16-17 3.670392400428066E-5 9.175981001070163E-6 0.0 8.870114967701159E-4 7.034918767487126E-5 18-19 4.282124467166077E-5 1.2234641334760218E-5 0.0 0.0012142881524749517 7.340784800856132E-5 20-21 4.893856533904087E-5 1.2234641334760218E-5 0.0 0.0015966206941862085 9.175981001070165E-5 22-23 4.893856533904087E-5 1.2234641334760218E-5 0.0 0.0022144700815915994 1.0093579101177181E-4 24-25 6.729052734118121E-5 1.2234641334760218E-5 0.0 0.003826384077446258 1.1622909268022207E-4 26-27 6.729052734118121E-5 1.2234641334760218E-5 0.0 0.0076282988722229965 1.1622909268022207E-4 28-29 1.0093579101177181E-4 1.2234641334760218E-5 0.0 0.026625638204771925 1.1622909268022207E-4 30-31 1.1622909268022207E-4 1.2234641334760218E-5 0.0 0.087404277695527 1.1928775301391214E-4 32-33 1.1928775301391214E-4 1.2234641334760218E-5 0.0 0.22617263837471108 1.223464133476022E-4 34-35 1.284637340149823E-4 1.835196200214033E-5 0.0 0.40140023023148064 1.4069837534974253E-4 36-37 1.4069837534974253E-4 2.446928266952044E-5 0.0 0.6768050653372668 1.4069837534974253E-4 38-39 1.6516765801926297E-4 3.058660333690055E-5 0.0 1.1065988394463924 1.4069837534974253E-4 40-41 1.6822631835295302E-4 3.058660333690055E-5 0.0 1.5776417068156618 1.4069837534974253E-4 42-43 1.896369406887834E-4 3.670392400428066E-5 0.0 2.0466811516663643 1.4681569601712264E-4 44-45 2.2634086469306404E-4 3.670392400428066E-5 0.0 2.5673783108927584 1.559916770181928E-4 46-47 2.8139675069948505E-4 3.670392400428066E-5 0.0 3.144324233655712 1.5905033735188286E-4 48-49 3.058660333690055E-4 3.670392400428066E-5 0.0 3.7266962198506324 1.6516765801926297E-4 50-51 3.058660333690055E-4 3.670392400428066E-5 0.0 4.3022993907270966 1.7740229935402318E-4 52-53 3.058660333690055E-4 3.670392400428066E-5 0.0 4.856672400227417 1.9269560102247343E-4 54-55 3.058660333690055E-4 4.282124467166077E-5 0.0 5.4298500534592655 2.1716488369199387E-4 56-57 3.3033531603852593E-4 4.893856533904087E-5 0.0 6.053975811869388 2.2328220435937398E-4 58-59 3.3645263670590604E-4 4.893856533904087E-5 0.0 6.692162231833485 2.2634086469306404E-4 60-61 3.4256995737328615E-4 4.893856533904087E-5 0.0 7.325760661297046 2.2634086469306404E-4 62-63 3.4256995737328615E-4 4.893856533904087E-5 0.0 7.941597450202869 2.3245818536044415E-4 64-65 3.4868727804066626E-4 4.893856533904087E-5 0.0 8.55811326170277 2.3857550602782428E-4 66-67 3.792738813775668E-4 4.893856533904087E-5 0.0 9.20794094289689 2.446928266952044E-4 68-69 3.91508522712327E-4 4.893856533904087E-5 0.0 9.893561067315854 2.477514870288945E-4 70-71 3.94567183046017E-4 5.199722567273093E-5 0.0 10.580572882186644 2.5386880769627453E-4 72-73 4.037431640470872E-4 5.505588600642098E-5 0.0 11.246678753676662 3.119833540363856E-4 74-75 4.098604847144673E-4 5.811454634011104E-5 0.0 11.909826900624005 3.242179953711458E-4 76-77 4.098604847144673E-4 6.423186700749115E-5 0.0 12.596086285052706 3.242179953711458E-4 78-79 4.098604847144673E-4 6.729052734118121E-5 0.0 13.30488493748037 3.242179953711458E-4 80-81 4.679750310545783E-4 6.729052734118121E-5 0.0 14.018614150365945 3.2727665570483585E-4 82-83 4.710336913882684E-4 6.729052734118121E-5 0.0 14.724577424664279 3.3645263670590604E-4 84-85 4.832683327230286E-4 7.340784800856132E-5 0.0 15.421673642655245 3.3645263670590604E-4 86-87 4.955029740577888E-4 7.340784800856132E-5 0.0 16.11973945597199 3.4562861770697623E-4 88-89 4.955029740577888E-4 7.340784800856132E-5 0.0 16.83966551469362 3.5480459870804637E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14585 0.0 45.06963 1 GTATCAA 25325 0.0 45.010735 1 ATCAACG 30970 0.0 35.903637 3 TCAACGC 31240 0.0 35.593002 4 TATCAAC 32370 0.0 34.688087 2 CAACGCA 32095 0.0 34.64556 5 AACGCAG 33235 0.0 33.542206 6 ACGCAGA 39980 0.0 27.741182 7 CGCAGAG 41125 0.0 26.864866 8 GTACATG 55940 0.0 25.378023 1 TACATGG 57525 0.0 24.29191 2 ACATGGG 58920 0.0 23.20904 3 GCAGAGT 49965 0.0 21.869081 9 CATGGGG 45770 0.0 21.58523 4 AGAGTAC 44295 0.0 18.73778 10-11 CAGAGTA 45715 0.0 18.337616 10-11 ATGGGGA 28165 0.0 18.213675 5 TCGCCGT 9665 0.0 16.894482 94-95 GTCCTAC 8895 0.0 16.76633 1 TAGGACG 9210 0.0 16.709354 4 >>END_MODULE