FastQCFastQC Report
Fri 27 May 2016
SRR940200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16347026
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158800.047.3160061
GTATCAA269850.044.4698561
ATCAACG325850.035.902743
TCAACGC330000.035.4803474
CAACGCA337100.034.7041365
TATCAAC345850.034.129622
AACGCAG351450.033.3411986
ACGCAGA423700.027.4539327
CGCAGAG432450.026.8874598
GTACATG562950.023.8155161
TACATGG577500.022.8822122
ACATGGG589450.022.2645973
GCAGAGT525150.022.0779469
CATGGGG461400.020.640574
AGAGTAC471800.018.91156810-11
CAGAGTA490750.018.82009910-11
ATGGGGA285050.017.412895
GAGTACT174250.016.87361312-13
TATAAGC71450.016.4865723
TAGGACG90450.016.2793854
Produced by FastQC (version 0.11.5)