FastQCFastQC Report
Fri 27 May 2016
SRR940199_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940199_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17233169
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163000.048.328271
GTATCAA284450.043.9459881
ATCAACG354100.035.945423
TCAACGC358050.035.548154
CAACGCA371300.034.3549775
TATCAAC364850.033.546112
AACGCAG384900.033.2775086
ACGCAGA446850.028.4413957
CGCAGAG460450.027.4671768
GTACATG524350.023.7311381
TACATGG538350.022.6465782
ACATGGG558200.022.4789143
GCAGAGT547600.022.3763459
CATGGGG429200.020.390034
AGAGTAC490800.019.6113310-11
CAGAGTA510650.018.98386610-11
GAGTACT205550.017.59685712-13
TCGCCGT107300.017.40952794-95
ATGGGGA253600.016.4480935
GTACTTT225600.016.23409814-15
Produced by FastQC (version 0.11.5)