FastQCFastQC Report
Fri 27 May 2016
SRR940199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17233169
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168750.050.992111
GTATCAA299650.043.9866141
ATCAACG370600.034.789113
TCAACGC371600.034.7210544
CAACGCA384900.033.5072945
TATCAAC391950.033.1973952
AACGCAG397950.032.429546
ACGCAGA469250.027.289247
CGCAGAG481700.026.356988
GTACATG529900.022.7396451
GCAGAGT571350.021.8056689
TACATGG552700.021.3761252
ACATGGG553100.020.6390153
AGAGTAC512000.019.19856610-11
CAGAGTA535150.018.79408510-11
CATGGGG421800.018.7294274
GAGTACT220950.017.89631312-13
GTACTTT237200.016.84085314-15
AGTACTT241550.016.0062912-13
ATGGGGA261050.015.2306775
Produced by FastQC (version 0.11.5)