FastQCFastQC Report
Fri 27 May 2016
SRR940198_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940198_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17571858
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159400.048.0944631
GTATCAA271300.044.9253431
ATCAACG347600.036.9024853
TCAACGC354150.036.2326624
CAACGCA364300.035.2478265
TATCAAC354850.033.9677352
AACGCAG379800.033.93336
ACGCAGA442150.028.8592727
CGCAGAG457800.027.6348348
GTACATG528900.023.5618611
ACATGGG568200.022.6171233
GCAGAGT548400.022.5828579
TACATGG544850.022.422062
CATGGGG429350.020.5069184
AGAGTAC488200.019.58559810-11
CAGAGTA508350.019.19226610-11
GAGTACT204400.018.07614512-13
TCGCCGT105450.017.25923294-95
GTACTTT223300.016.35643414-15
ATGGGGA260350.015.9600895
Produced by FastQC (version 0.11.5)