FastQCFastQC Report
Fri 27 May 2016
SRR940198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940198_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17571858
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168000.053.5940741
GTATCAA310100.045.1232451
ATCAACG378200.036.212493
TCAACGC380750.036.007294
CAACGCA391650.035.0506945
TATCAAC399600.034.6424942
AACGCAG408550.033.670366
ACGCAGA476800.028.6213517
CGCAGAG491650.027.7659688
GTACATG532500.023.689821
GCAGAGT588150.022.8711099
TACATGG546800.022.5017552
ACATGGG560950.021.0952573
AGAGTAC524150.019.9229210-11
CAGAGTA545750.019.70447210-11
CATGGGG424900.019.3416424
GAGTACT239000.018.94997212-13
GTACTTT253300.017.796114-15
AGTACTT260700.016.8989112-13
ATGGGGA266300.015.2329375