FastQCFastQC Report
Fri 27 May 2016
SRR940197_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940197_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16437750
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151200.050.0174521
GTATCAA267550.043.8020551
ATCAACG329700.035.4411773
TCAACGC333700.035.0867734
CAACGCA340250.034.354975
TATCAAC345650.033.5867922
AACGCAG358300.032.745486
ACGCAGA420650.027.631967
CGCAGAG430700.026.8212728
GTACATG501100.025.027061
TACATGG519850.023.592942
ACATGGG529650.022.8060133
GCAGAGT512050.022.1140679
CATGGGG404000.020.7866084
AGAGTAC457250.019.43044510-11
CAGAGTA476150.018.96842610-11
CGTATCA37050.017.83235794-95
GAGTACT198200.017.49528712-13
TCGCCGT105500.017.25553994-95
ATGGGGA249050.016.859395
Produced by FastQC (version 0.11.5)