FastQCFastQC Report
Fri 27 May 2016
SRR940197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16437750
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163300.051.3531041
GTATCAA288150.044.0416341
TCAACGC353000.035.0662164
ATCAACG353300.035.036233
CAACGCA365450.033.884295
TATCAAC373100.033.446082
AACGCAG379700.032.7626386
ACGCAGA444100.027.819137
CGCAGAG456300.026.8879628
GTACATG511150.024.0374451
TACATGG527050.022.7752822
GCAGAGT546900.022.1208089
ACATGGG537900.021.6170523
CATGGGG409650.019.9668454
AGAGTAC489550.019.65226210-11
CAGAGTA512950.019.09373910-11
GAGTACT220350.018.31318912-13
GTACTTT230750.017.44676614-15
AGTACTT236950.016.02785712-13
ATGGGGA251100.015.7762815
Produced by FastQC (version 0.11.5)