FastQCFastQC Report
Fri 27 May 2016
SRR940196_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940196_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16603486
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157400.049.729061
GTATCAA277700.044.109611
ATCAACG335050.035.568313
TCAACGC337000.035.30584
CAACGCA347550.034.2760125
TATCAAC354600.033.8892362
AACGCAG362350.032.928566
ACGCAGA423850.027.9046347
CGCAGAG437150.026.9687298
GTACATG513300.024.2245751
TACATGG533350.022.887632
GCAGAGT519400.022.4191049
ACATGGG538150.022.0299453
CATGGGG414500.020.4541744
AGAGTAC466250.019.27896510-11
CAGAGTA486900.018.99798810-11
GAGTACT198650.017.62730412-13
TCGCCGT98250.016.83757694-95
ATGGGGA251350.016.2328325
GTCCTAC67900.016.228411
Produced by FastQC (version 0.11.5)