FastQCFastQC Report
Fri 27 May 2016
SRR940196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940196_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16603486
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159900.051.1853831
GTATCAA291450.045.4174161
ATCAACG353750.036.7366563
TCAACGC357250.036.4032254
CAACGCA365350.035.583045
TATCAAC371300.035.218992
AACGCAG378900.034.3605776
ACGCAGA448400.028.8757767
CGCAGAG460650.028.1388198
GTACATG515750.024.4212511
GCAGAGT546850.023.2863839
TACATGG533700.023.220552
ACATGGG535900.022.3089643
CATGGGG405800.020.0270354
AGAGTAC487400.019.9776210-11
CAGAGTA507850.019.65015610-11
GAGTACT216800.018.9187412-13
GTACTTT232100.017.57003214-15
AGTACTT241000.016.45729812-13
ATGGGGA254550.015.7860915
Produced by FastQC (version 0.11.5)