FastQCFastQC Report
Fri 27 May 2016
SRR940195_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940195_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12366398
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104550.050.673081
GTATCAA197350.045.1189421
ATCAACG246850.037.1291473
TCAACGC247500.037.1075254
CAACGCA254850.036.0166445
AACGCAG263300.035.0463526
TATCAAC257250.034.1514972
ACGCAGA310000.029.5223857
CGCAGAG320500.028.480188
GTACATG390000.024.1105791
GCAGAGT378700.023.5140179
ACATGGG413650.022.5822073
TACATGG407150.022.5699332
CATGGGG327700.020.2836744
AGAGTAC339800.019.82738710-11
CAGAGTA354700.019.47655310-11
GAGTACT147650.017.17904312-13
TCGCCGT77700.017.12942394-95
TAGGACG65200.016.9790614
ATGGGGA198950.016.6205755
Produced by FastQC (version 0.11.5)