##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940195_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12366398 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.634481520002833 33.0 31.0 34.0 28.0 34.0 2 30.616265463880428 33.0 31.0 34.0 25.0 34.0 3 31.46513819141192 34.0 31.0 34.0 28.0 34.0 4 35.21340110515609 37.0 35.0 37.0 33.0 37.0 5 35.37621836204851 37.0 35.0 37.0 33.0 37.0 6 35.525167716581656 37.0 35.0 37.0 35.0 37.0 7 35.49211306315711 37.0 36.0 37.0 35.0 37.0 8 35.483313087610476 37.0 36.0 37.0 35.0 37.0 9 37.201425750651076 39.0 38.0 39.0 35.0 39.0 10-11 37.126326598901315 39.0 38.0 39.0 34.0 39.0 12-13 37.04415740137104 39.0 38.0 39.0 34.0 39.0 14-15 38.41511760336357 41.0 39.0 41.0 34.0 41.0 16-17 38.32596961540458 40.5 38.0 41.0 34.0 41.0 18-19 38.25781585713156 40.0 38.5 41.0 34.0 41.0 20-21 38.17915847444017 40.0 38.0 41.0 34.0 41.0 22-23 38.068991916643796 40.0 38.0 41.0 33.5 41.0 24-25 37.95503379399563 40.0 38.0 41.0 33.0 41.0 26-27 37.75200701125744 40.0 38.0 41.0 33.0 41.0 28-29 37.57868714075028 40.0 38.0 41.0 32.0 41.0 30-31 37.55171784864113 40.0 38.0 41.0 32.5 41.0 32-33 37.569466994350336 40.0 38.0 41.0 32.5 41.0 34-35 37.57692243933925 40.0 38.0 41.0 32.5 41.0 36-37 37.67509358828658 40.0 38.0 41.0 33.0 41.0 38-39 37.58587759345931 40.0 38.0 41.0 33.0 41.0 40-41 37.448734344471205 40.0 38.0 41.0 32.0 41.0 42-43 37.171753650497095 40.0 38.0 41.0 31.0 41.0 44-45 37.11535707487337 40.0 37.5 41.0 31.0 41.0 46-47 36.95053620302372 40.0 37.0 41.0 31.0 41.0 48-49 36.76355241032999 40.0 37.0 41.0 30.5 41.0 50-51 35.83151484369175 39.0 36.0 40.0 29.0 40.5 52-53 35.91499925847445 39.0 35.5 40.0 29.5 40.5 54-55 36.28899183092765 40.0 36.0 41.0 30.0 41.0 56-57 36.1178851351865 39.5 35.5 41.0 29.5 41.0 58-59 35.854279030967625 39.0 35.0 41.0 29.0 41.0 60-61 35.58709848251689 39.0 35.0 41.0 28.5 41.0 62-63 35.24398438413513 38.0 35.0 40.5 28.0 41.0 64-65 34.85883908960394 37.5 35.0 40.0 28.0 41.0 66-67 34.47290140589038 37.0 34.0 40.0 26.5 41.0 68-69 34.05963413922146 36.5 34.0 39.0 26.0 41.0 70-71 33.60643365998733 36.0 34.0 39.0 26.0 41.0 72-73 33.12654238526045 35.0 33.5 38.5 25.5 40.0 74-75 32.64865820265529 35.0 33.0 37.0 24.0 39.5 76-77 32.15217697182317 35.0 33.0 37.0 22.5 39.0 78-79 31.667478517188272 35.0 32.5 36.0 20.0 39.0 80-81 31.220934018135274 35.0 32.0 36.0 18.5 37.0 82-83 30.806993839273165 35.0 31.5 35.0 15.5 37.0 84-85 30.441881823632073 35.0 31.0 35.0 9.0 36.5 86-87 30.059193833159824 34.0 31.0 35.0 4.5 36.0 88-89 29.654876019678486 34.0 30.5 35.0 2.0 36.0 90-91 29.2717104447067 34.0 30.0 35.0 2.0 35.0 92-93 28.86269073662355 34.0 29.5 35.0 2.0 35.0 94-95 28.408624443431304 34.0 29.0 35.0 2.0 35.0 96-97 27.884784316338518 34.0 29.0 35.0 2.0 35.0 98-99 27.241128419124145 34.0 27.0 35.0 2.0 35.0 100-101 25.1169563279461 31.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6592269811714502 1101 2 1.7475394264942743 1101 3 1.026523438133463 1101 4 0.6448245909340287 1101 5 0.484317784435774 1101 6 0.4378885969486177 1101 7 0.4523112314595963 1101 8 0.46017851878871596 1101 9 0.4984459666873562 1101 10-11 0.48153025320940657 1101 12-13 0.5242222758746564 1101 14-15 0.5915609384332754 1101 16-17 0.585653786251882 1101 18-19 0.6040374295092334 1101 20-21 0.650990022894355 1101 22-23 0.5778614015286863 1101 24-25 0.5843866997188272 1101 26-27 0.6717196473442044 1101 28-29 0.6755055583660123 1101 30-31 0.6252804707881765 1101 32-33 0.670583212114984 1101 34-35 0.7020347604945343 1101 36-37 0.7514466971558633 1101 38-39 0.7930126322826467 1101 40-41 0.7684128389998222 1101 42-43 0.8534437318920141 1101 44-45 0.7867940714273018 1101 46-47 0.761655357642141 1101 48-49 0.7763242883106898 1101 50-51 0.5589432487335557 1101 52-53 0.6749043282211034 1101 54-55 0.7691579517529945 1101 56-57 0.8581501887093026 1101 58-59 0.8817233108497788 1101 60-61 0.7884578565522204 1101 62-63 0.8625935444500712 1101 64-65 0.8707323674669851 1101 66-67 0.8645035519023168 1101 68-69 0.7649333925367614 1101 70-71 0.6971582847263207 1101 72-73 0.7410766130017947 1101 74-75 0.679094041946712 1101 76-77 0.6771243696707572 1101 78-79 0.6455123357813086 1101 80-81 0.6405698308222512 1101 82-83 0.6874526297318475 1101 84-85 0.6069781917552355 1101 86-87 0.5670325591930876 1101 88-89 0.5677501542534991 1101 90-91 0.5057638080630973 1101 92-93 0.4893126109288488 1101 94-95 0.43450331035700884 1101 96-97 0.3682876187628139 1101 98-99 0.3416012653783014 1101 100-101 0.4915223119389971 1102 1 0.38894507631893305 1102 2 0.888623376896966 1102 3 0.5492891115881982 1102 4 0.3493402427963517 1102 5 0.3314451183556315 1102 6 0.24323574766752643 1102 7 0.25958480061648004 1102 8 0.29359971924689177 1102 9 0.2791481496260175 1102 10-11 0.3240471793128208 1102 12-13 0.36105326776604585 1102 14-15 0.3971088451131948 1102 16-17 0.39636113664810324 1102 18-19 0.3945522469289813 1102 20-21 0.4289750747900385 1102 22-23 0.413027188915315 1102 24-25 0.4313611657713565 1102 26-27 0.45996503865335825 1102 28-29 0.41277273101054845 1102 30-31 0.4174009220728365 1102 32-33 0.36227829218494634 1102 34-35 0.39046260723121406 1102 36-37 0.45603649100818444 1102 38-39 0.4115003936894652 1102 40-41 0.4532536546392265 1102 42-43 0.6130059615804342 1102 44-45 0.37259164144018797 1102 46-47 0.41223952593465896 1102 48-49 0.38451902225340007 1102 50-51 0.2484420101250251 1102 52-53 0.2756798495370205 1102 54-55 0.43772227482157433 1102 56-57 0.4652886668868845 1102 58-59 0.4733379612051323 1102 60-61 0.48455453882132105 1102 62-63 0.4585632140454692 1102 64-65 0.4639327682801877 1102 66-67 0.41942406486892025 1102 68-69 0.3702730450983225 1102 70-71 0.3613850049392582 1102 72-73 0.3154461420755723 1102 74-75 0.24253399201607806 1102 76-77 0.22101634816954885 1102 78-79 0.30528135290331804 1102 80-81 0.23260868970838544 1102 82-83 0.24643460215453317 1102 84-85 0.1230418001631648 1102 86-87 0.05171052586884883 1102 88-89 0.0753157727397884 1102 90-91 -0.01431682951806934 1102 92-93 -0.03274198302591813 1102 94-95 -0.11881776017581558 1102 96-97 -0.13178776469666786 1102 98-99 -0.16052569017189455 1102 100-101 -0.03668892415723235 1103 1 0.316071983492769 1103 2 1.3385428155578545 1103 3 0.6508362132760617 1103 4 0.2890373628211833 1103 5 0.2234528767969195 1103 6 0.1517384096471588 1103 7 0.11911076584903668 1103 8 0.1584612886098924 1103 9 0.18627401814958944 1103 10-11 0.16211658743484492 1103 12-13 0.22757778695661557 1103 14-15 0.23300103653615167 1103 16-17 0.2961659172570492 1103 18-19 0.20099697329475674 1103 20-21 0.21796326946071787 1103 22-23 0.22219123092383342 1103 24-25 0.18956891950195143 1103 26-27 0.23320828291144124 1103 28-29 0.21675387483788455 1103 30-31 0.23320258164169871 1103 32-33 0.17923100867898967 1103 34-35 0.2510363462176244 1103 36-37 0.2595129724533436 1103 38-39 0.21724670766025156 1103 40-41 0.3501028243427555 1103 42-43 0.4407744967397278 1103 44-45 0.3139738062728483 1103 46-47 0.1653618499827445 1103 48-49 0.17893653852688374 1103 50-51 0.20849183430545537 1103 52-53 0.2560346618702667 1103 54-55 0.3228531956478804 1103 56-57 0.3991374752816341 1103 58-59 0.34716726860757063 1103 60-61 0.35709075457308614 1103 62-63 0.2448655807778266 1103 64-65 0.2718796003099868 1103 66-67 0.23038290896506197 1103 68-69 0.22592621336643504 1103 70-71 0.25116953745385473 1103 72-73 0.30085827272865373 1103 74-75 0.3325759628585274 1103 76-77 0.3070869208708231 1103 78-79 0.31722158728512895 1103 80-81 0.2651096725005573 1103 82-83 0.318287295572965 1103 84-85 0.20899815081596174 1103 86-87 0.0781441518344721 1103 88-89 0.07767099057177873 1103 90-91 0.010399846581375272 1103 92-93 0.09480226015491411 1103 94-95 0.08511424869792705 1103 96-97 -0.051929826420234804 1103 98-99 -0.04739816557990295 1103 100-101 -0.08643276203171624 1104 1 0.4516042136543703 1104 2 1.4679717024522283 1104 3 0.7668471773884242 1104 4 0.46397722215240833 1104 5 0.35088950849567624 1104 6 0.23447442033883448 1104 7 0.25378468037315827 1104 8 0.2358758301347308 1104 9 0.21208547446644133 1104 10-11 0.18830407996171772 1104 12-13 0.24690088469674976 1104 14-15 0.22109077380005715 1104 16-17 0.24749455910866658 1104 18-19 0.19551461819010285 1104 20-21 0.2840301703735406 1104 22-23 0.19808867924008666 1104 24-25 0.2440172138135992 1104 26-27 0.2731430567350017 1104 28-29 0.2560066949486455 1104 30-31 0.09923726298772806 1104 32-33 0.20639602734855345 1104 34-35 0.19442700250541378 1104 36-37 0.3052756813034847 1104 38-39 0.3783887408946498 1104 40-41 0.3282799384987811 1104 42-43 0.4109755786209419 1104 44-45 0.26415279041241746 1104 46-47 0.289412415215736 1104 48-49 0.31633896791626626 1104 50-51 0.25831727728193243 1104 52-53 0.36691507903324805 1104 54-55 0.5096026405556344 1104 56-57 0.46966417344010836 1104 58-59 0.46633455456596806 1104 60-61 0.40383578750023474 1104 62-63 0.4200160005592366 1104 64-65 0.3823299855826008 1104 66-67 0.4121247823692187 1104 68-69 0.246337518934304 1104 70-71 0.24687517056172226 1104 72-73 0.2221544112239755 1104 74-75 0.22580071202645513 1104 76-77 0.18204110211165414 1104 78-79 0.21478283662484898 1104 80-81 0.1290139537109667 1104 82-83 0.07550883375740725 1104 84-85 -0.011868356878139252 1104 86-87 -0.11327807243944221 1104 88-89 -0.07207501405058636 1104 90-91 -0.06899223470804472 1104 92-93 -0.05349581264957948 1104 94-95 -0.14035283474829185 1104 96-97 -0.30144206417982744 1104 98-99 -0.29722117175062124 1104 100-101 -0.2854911257998971 1105 1 0.20211681447139895 1105 2 1.1880183846492116 1105 3 0.4844288643895851 1105 4 0.26620836837554407 1105 5 0.14597513966799625 1105 6 0.023588745185243454 1105 7 -0.012348590181929353 1105 8 0.014531371911921553 1105 9 -0.12786264223174726 1105 10-11 -0.09529659928431755 1105 12-13 -0.2106261998285106 1105 14-15 -0.1630090290141908 1105 16-17 -0.32485341122860945 1105 18-19 -0.24581148104515194 1105 20-21 -0.15384297924217805 1105 22-23 -0.23187745883009114 1105 24-25 -0.22617925107757486 1105 26-27 -0.15722694256239578 1105 28-29 -0.17392177617291082 1105 30-31 -0.2129789277778542 1105 32-33 -0.28132341593249066 1105 34-35 -0.19430357205241933 1105 36-37 -0.17003045846389142 1105 38-39 -0.13747092417244744 1105 40-41 -0.20161716174840194 1105 42-43 -0.0809362471402224 1105 44-45 -0.12006375439327854 1105 46-47 -0.21177427616074596 1105 48-49 -0.32377821206294755 1105 50-51 -0.34820538265432077 1105 52-53 -0.3495092252733585 1105 54-55 -0.18392565208589673 1105 56-57 -0.2303033972150743 1105 58-59 -0.2788262006344553 1105 60-61 -0.4688516963603675 1105 62-63 -0.37055669583037343 1105 64-65 -0.32985755348271084 1105 66-67 -0.40383950702755556 1105 68-69 -0.5240851848919732 1105 70-71 -0.5070719843883111 1105 72-73 -0.6853992171853704 1105 74-75 -0.7979320752821195 1105 76-77 -0.7845880983507882 1105 78-79 -0.6745865066033616 1105 80-81 -0.7230519889984066 1105 82-83 -0.6932023598414006 1105 84-85 -0.7070593788587374 1105 86-87 -0.7977959528341074 1105 88-89 -0.9118969902696712 1105 90-91 -1.0627480437490213 1105 92-93 -1.0849643582837984 1105 94-95 -1.1824092968472897 1105 96-97 -1.3540133170347346 1105 98-99 -1.523292521784061 1105 100-101 -1.4977068238292226 1106 1 0.19272538189499855 1106 2 1.2230767081752845 1106 3 0.48055109663843965 1106 4 0.23724445875188138 1106 5 0.0441898492870223 1106 6 0.11791082845027745 1106 7 0.13242329341039039 1106 8 0.12058915409345872 1106 9 0.00859336103574293 1106 10-11 0.09321223696299796 1106 12-13 0.051224255470671665 1106 14-15 0.07230299053083655 1106 16-17 -0.0309801279676023 1106 18-19 -0.08987225764465734 1106 20-21 -0.19038677696300255 1106 22-23 -0.15304008717419748 1106 24-25 -0.08342806727396379 1106 26-27 -0.0029896024722617653 1106 28-29 -0.10522111887277674 1106 30-31 -0.111957755672762 1106 32-33 -0.05046178193310169 1106 34-35 -0.09058079005865949 1106 36-37 -0.10632095795512697 1106 38-39 -0.09769865624539875 1106 40-41 -0.07239943910890645 1106 42-43 0.12273847253819525 1106 44-45 -0.05591570280655134 1106 46-47 -0.14494733547804373 1106 48-49 -0.23265348953565024 1106 50-51 -0.45911407747581734 1106 52-53 -0.2370989457877215 1106 54-55 -0.12351047932304482 1106 56-57 -0.006884612875758478 1106 58-59 -0.16458616025649064 1106 60-61 -0.14382654495764058 1106 62-63 -0.11574635185606041 1106 64-65 -0.2973870018880902 1106 66-67 -0.18379242046613342 1106 68-69 -0.16580490086568744 1106 70-71 -0.3456094322208756 1106 72-73 -0.2434671167884801 1106 74-75 -0.29082294750199367 1106 76-77 -0.3934128214747652 1106 78-79 -0.4336318565802024 1106 80-81 -0.529502857273151 1106 82-83 -0.4044036287426849 1106 84-85 -0.4121031725431763 1106 86-87 -0.4824627989739767 1106 88-89 -0.7098483165286744 1106 90-91 -0.8631009765154829 1106 92-93 -1.0015067918210931 1106 94-95 -0.9584379713768492 1106 96-97 -1.0201089804637462 1106 98-99 -1.0050979637064081 1106 100-101 -0.9774569114891314 1107 1 0.4148485532508346 1107 2 1.4519285412184821 1107 3 0.7237222462229305 1107 4 0.41534564798391216 1107 5 0.2937705532164614 1107 6 0.28929772898563044 1107 7 0.2685426477310955 1107 8 0.24018705207231505 1107 9 0.1898954063087217 1107 10-11 0.3268020500268065 1107 12-13 0.3494533691706252 1107 14-15 0.3080326005481169 1107 16-17 0.37809609494842533 1107 18-19 0.40984528613915927 1107 20-21 0.3227737755951239 1107 22-23 0.281187598317608 1107 24-25 0.2565342196154532 1107 26-27 0.3202491573010562 1107 28-29 0.27898528920294297 1107 30-31 0.23865851504855584 1107 32-33 0.21957903592312533 1107 34-35 0.33351869805805734 1107 36-37 0.36840330699845225 1107 38-39 0.34179551311737555 1107 40-41 0.25624970512142653 1107 42-43 0.41275085738493544 1107 44-45 0.17239228321873412 1107 46-47 0.12620868272491492 1107 48-49 0.1786673902839766 1107 50-51 0.15120555645705025 1107 52-53 0.2327728852238522 1107 54-55 0.23640899258308679 1107 56-57 0.29303077153149104 1107 58-59 0.3532543458639452 1107 60-61 0.27637505995205913 1107 62-63 0.21958755355823456 1107 64-65 0.1380597827068044 1107 66-67 0.24355037342120056 1107 68-69 0.15261355309135638 1107 70-71 0.013409585293949533 1107 72-73 0.08387793904825713 1107 74-75 0.05856129740773497 1107 76-77 0.09722079906325831 1107 78-79 0.07859053045231335 1107 80-81 0.08703127604647065 1107 82-83 0.10689633568057033 1107 84-85 -0.029877129021834037 1107 86-87 0.07393881070036912 1107 88-89 0.11956803099471713 1107 90-91 0.11339780032504265 1107 92-93 -0.061496950993699784 1107 94-95 0.0021015621268958284 1107 96-97 0.0586457917734009 1107 98-99 0.08510235338333061 1107 100-101 -0.04001995726527596 1108 1 0.21636995030905481 1108 2 1.3143006911531963 1108 3 0.5673972901884632 1108 4 0.2225693498872161 1108 5 0.10379537241551873 1108 6 0.04788826759924092 1108 7 -0.004964169494641624 1108 8 0.031561320822966366 1108 9 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-2.7875101848475765 2316 60-61 -2.7913718323266394 2316 62-63 -2.476126259762246 2316 64-65 -2.265926735674384 2316 66-67 -2.395058516343717 2316 68-69 -2.3170240925683245 2316 70-71 -2.2229632993669526 2316 72-73 -2.281277195480122 2316 74-75 -2.2936399961189196 2316 76-77 -2.3268699579087695 2316 78-79 -2.5633723899156173 2316 80-81 -2.508247568448855 2316 82-83 -2.6312062182108598 2316 84-85 -2.118188768410409 2316 86-87 -1.9601064361516123 2316 88-89 -1.99477430830796 2316 90-91 -1.7996621746266435 2316 92-93 -2.099844873043118 2316 94-95 -2.0501914860695187 2316 96-97 -1.7665093154700529 2316 98-99 -1.6721083602315794 2316 100-101 -1.7684487202666972 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 135762.0 3 34300.0 4 26227.0 5 20775.0 6 18717.0 7 18349.0 8 19550.0 9 22080.0 10 24775.0 11 28580.0 12 31407.0 13 34369.0 14 37642.0 15 42570.0 16 49579.0 17 58223.0 18 55202.0 19 48768.0 20 51389.0 21 55592.0 22 59792.0 23 66990.0 24 76101.0 25 88696.0 26 102053.0 27 120309.0 28 141997.0 29 170746.0 30 206883.0 31 254702.0 32 321844.0 33 423784.0 34 595195.0 35 904731.0 36 1600603.0 37 2959645.0 38 3009898.0 39 448531.0 40 42.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.505786121641854 18.75107965125899 14.126066048158817 24.617068178940336 2 16.636230336365475 20.795391078459062 38.31561672182316 24.252761863352298 3 18.289189730872625 25.041911197537576 29.61375349152095 27.055145580068857 4 13.347811329166595 17.186273791901197 36.9775592413131 32.48835563761911 5 14.848194349552541 36.582590371525676 34.001146686180505 14.568068592741279 6 31.999224587655807 35.49918261445945 18.37655278586375 14.125040012020996 7 28.645209502142777 31.51066051263305 21.965144575848033 17.87898540937614 8 26.632579620932923 34.182589855781245 20.66878890485829 18.516041618427547 9 27.099000546594404 16.204069673925183 19.885830589376596 36.81109919010382 10-11 25.308406593833176 25.619061467651292 27.866273923963497 21.20625801455203 12-13 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25.743639736928237 26.208306519283042 24.03284098200874 38-39 23.896954418274017 25.748176890682988 26.45487752631729 23.899991164725705 40-41 24.04830985776528 25.422472882043216 26.69249564134536 23.836721618846145 42-43 23.80348751877695 25.573611872451153 26.56169151146787 24.06120909730403 44-45 23.56051411140461 25.708963269922723 26.51024228809437 24.220280330578298 46-47 23.722048039071733 25.635770745849452 26.50147221414918 24.140709000929633 48-49 23.612964352458704 25.738698732409205 26.398659560086774 24.24967735504532 50-51 23.29873312285909 25.882355510176964 26.530361859585373 24.288549507378576 52-53 23.484113787872715 25.744916839538114 26.41482409854556 24.356145274043612 54-55 23.2754168609589 25.550014823627944 26.648584751929455 24.525983563483702 56-57 23.28151850136127 25.760356226902413 26.549952413794887 24.40817285794143 58-59 23.499403700417698 25.524242419708614 26.371548683403763 24.604805196469925 60-61 23.37757779889317 25.299649547212866 26.702382663258405 24.62038999063556 62-63 23.409707295199812 25.32982833187316 26.66223223563911 24.598232137287916 64-65 23.350447848781915 25.334179084355092 26.45826743595179 24.857105630911203 66-67 23.302037016525365 25.610287478775977 26.22194685809299 24.86572864660566 68-69 23.25632681601807 25.290367353987257 26.538799463494815 24.91450636649985 70-71 23.255176512417062 25.20611259227914 26.43224958419995 25.106461311103846 72-73 23.214420184148896 25.231403424553108 26.386311493675063 25.167864897622938 74-75 23.102233311965694 25.308865391317404 26.510200485836037 25.07870081088086 76-77 23.22559033454094 25.087279842525945 26.609056812993416 25.0780730099397 78-79 23.130633000047688 25.04882231669785 26.674423553269666 25.1461211299848 80-81 23.152840699286507 25.105366890219187 26.762484441295353 24.979307969198953 82-83 23.110874733802568 25.03908596388684 26.81008795160757 25.03995135070302 84-85 23.169001186169197 24.919610760505076 26.799048557312766 25.112339496012957 86-87 23.271117108028964 24.87099814387448 26.847749985683535 25.01013476241302 88-89 23.310750122313145 24.642723890480863 26.862862154278833 25.18366383292716 90-91 23.440069040023293 24.650477602455048 26.93167948737807 24.97777387014359 92-93 23.541819592196607 24.51469496863309 26.91216949983249 25.031315939337812 94-95 23.511426591480753 24.34508046494761 26.93326568877715 25.210227254794486 96-97 23.731551920185908 24.192255378944267 26.78659630921649 25.28959639165334 98-99 23.660614065543996 24.03260416420889 26.844345849654484 25.46243592059263 100-101 23.681204092342632 23.899799411551648 27.098936506114303 25.320059989991417 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 279.0 1 221.0 2 260.5 3 348.5 4 321.5 5 357.0 6 532.5 7 1036.0 8 1871.0 9 2369.0 10 2383.5 11 2414.0 12 2593.0 13 2843.5 14 3138.5 15 3489.5 16 3782.5 17 4072.0 18 4542.0 19 5060.5 20 5747.0 21 6667.0 22 7976.5 23 9810.0 24 11986.0 25 15354.5 26 19959.5 27 26027.5 28 33813.0 29 44255.5 30 58919.5 31 78302.0 32 100273.0 33 124762.0 34 155226.5 35 185163.5 36 214786.0 37 244344.0 38 269667.0 39 287696.5 40 306405.0 41 338606.0 42 372518.0 43 406597.0 44 440256.0 45 465911.5 46 487832.5 47 512365.0 48 541871.0 49 561721.5 50 570069.5 51 570242.5 52 559459.0 53 549215.5 54 534806.5 55 502138.5 56 460742.0 57 414593.5 58 366612.5 59 318727.5 60 264033.0 61 210184.5 62 165826.0 63 128687.0 64 97406.5 65 73680.0 66 54745.5 67 41220.0 68 29994.0 69 20890.5 70 15283.5 71 10987.0 72 7916.5 73 5796.5 74 4357.0 75 3229.5 76 2244.5 77 1518.0 78 1036.0 79 740.5 80 532.5 81 410.0 82 336.0 83 268.5 84 225.0 85 189.0 86 169.0 87 163.5 88 150.0 89 133.0 90 118.5 91 108.5 92 95.5 93 96.0 94 91.5 95 80.0 96 76.0 97 59.0 98 43.5 99 35.5 100 28.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05727617694335893 2 0.05431654391197825 3 0.06088272429853867 4 0.05994469852903004 5 0.059079450620948805 6 0.011029889220773907 7 0.013002977908361028 8 0.01438575727548151 9 0.005975871066093781 10-11 0.0112401363760086 12-13 0.01481029480047464 14-15 0.017078538148295082 16-17 0.0317675365130574 18-19 0.030437318934745588 20-21 0.030065343198561134 22-23 0.018873725396837463 24-25 0.007492076512497818 26-27 0.0107266481314931 28-29 0.017555637462096884 30-31 0.015647240206889672 32-33 0.018186378927800964 34-35 0.023341477445574695 36-37 0.0218293152136944 38-39 0.009626893781034703 40-41 0.010868160639824143 42-43 0.012161989287422254 44-45 0.02306249564343635 46-47 0.015028628384756822 48-49 0.014559615500002506 50-51 0.014405973348100232 52-53 0.011947698917663818 54-55 0.008118774763678154 56-57 0.011887050699807656 58-59 0.011228006732437366 60-61 0.012428841445989366 62-63 0.020555702638715007 64-65 0.014919461592615731 66-67 0.017494989244240724 68-69 0.02466765180936276 70-71 0.018453231086368073 72-73 0.024526139301031716 74-75 0.0406423923926757 76-77 0.048619654648022814 78-79 0.0362231589182234 80-81 0.018910114327551162 82-83 0.01605156165926408 84-85 0.021408820903225014 86-87 0.024340151432939484 88-89 0.02135625911441634 90-91 0.025346911849351766 92-93 0.04915740217968078 94-95 0.07804212673730863 96-97 0.0928928536830207 98-99 0.09624872173772832 100-101 0.08089259297654822 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2366398E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.24608532571032 #Duplication Level Percentage of deduplicated Percentage of total 1 75.97331159642118 35.89441562161292 2 12.435827043759042 11.750882912104311 3 4.0249703539931625 5.704922783346463 4 2.047720582259956 3.8698712541066835 5 1.1726607669375386 2.7701815326421926 6 0.7892154977276559 2.237240564760826 7 0.5687004164091847 1.880820787890473 8 0.42458572712107323 1.6048010793312786 9 0.3125580401411507 1.3290429450371037 >10 2.0038325416158327 17.614282051974595 >50 0.1551685915976357 5.041846376971755 >100 0.08485664915501949 7.3953980966460735 >500 0.004966030244224699 1.585100712213971 >1k 0.0015915641081753963 1.1856976307451554 >5k 3.459850912117637E-5 0.1354956506162127 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.4259287142464604E-5 2 0.0 0.0 0.0 0.0 3.2345716189952805E-5 3 0.0 0.0 0.0 3.2345716189952805E-5 3.2345716189952805E-5 4 0.0 0.0 0.0 7.277786142739381E-5 3.2345716189952805E-5 5 0.0 0.0 0.0 7.277786142739381E-5 3.2345716189952805E-5 6 0.0 0.0 0.0 8.895071952237021E-5 4.0432145237441006E-5 7 0.0 0.0 0.0 1.0512357761734662E-4 4.0432145237441006E-5 8 0.0 0.0 0.0 1.1321000666483482E-4 4.0432145237441006E-5 9 0.0 8.086429047488201E-6 0.0 1.6981500999725224E-4 4.0432145237441006E-5 10-11 0.0 8.086429047488201E-6 0.0 2.506793004721342E-4 4.851857428492921E-5 12-13 0.0 8.086429047488201E-6 0.0 3.3558680547076037E-4 7.277786142739381E-5 14-15 0.0 2.0216072618720503E-5 0.0 4.5284002665933927E-4 8.490750499862612E-5 16-17 0.0 2.4259287142464604E-5 0.0 5.741364623716623E-4 9.299393404611431E-5 18-19 8.086429047488201E-6 2.4259287142464604E-5 0.0 7.196921852264499E-4 1.1725322118857892E-4 20-21 1.6172858094976402E-5 2.4259287142464604E-5 0.0 9.016368387949345E-4 1.4151250833104354E-4 22-23 1.6172858094976402E-5 2.4259287142464604E-5 0.0 0.0014312979414054117 1.5768536642601992E-4 24-25 4.0432145237441006E-5 2.4259287142464604E-5 0.0 0.002531052291863807 1.6981500999725224E-4 26-27 4.0432145237441006E-5 3.2345716189952805E-5 0.0 0.004819511712302969 1.6981500999725224E-4 28-29 5.660500333241741E-5 4.0432145237441006E-5 0.0 0.019439775430161635 1.6981500999725224E-4 30-31 9.703714856985841E-5 4.0432145237441006E-5 0.0 0.07745586063136574 1.9811751166346094E-4 32-33 9.703714856985841E-5 4.0432145237441006E-5 0.0 0.20285615908528903 2.0216072618720504E-4 34-35 1.2129643571232303E-4 4.0432145237441006E-5 0.0 0.35240253467501204 2.1833358428218145E-4 36-37 1.4555572285478762E-4 4.0432145237441006E-5 0.0 0.5865612606031279 2.1833358428218145E-4 38-39 1.7790143904474043E-4 4.447535976118511E-5 0.0 0.9710103135933357 2.1833358428218145E-4 40-41 1.7790143904474043E-4 4.851857428492921E-5 0.0 1.4081747975441192 2.1833358428218145E-4 42-43 2.1429036975843734E-4 4.851857428492921E-5 0.0 1.8341597933367502 2.2642001332966963E-4 44-45 2.3450644237715785E-4 4.851857428492921E-5 0.0 2.293925846475263 2.3450644237715785E-4 46-47 2.9111144570957524E-4 4.851857428492921E-5 0.0 2.807106806686959 2.466360859483901E-4 48-49 3.3154359094701626E-4 4.851857428492921E-5 0.0 3.3357854081681664 2.6685215856711065E-4 50-51 3.3154359094701626E-4 4.851857428492921E-5 0.0 3.8792581315917536 2.6685215856711065E-4 52-53 3.3154359094701626E-4 4.851857428492921E-5 0.0 4.396458047040052 2.830250166620871E-4 54-55 3.6388930713696907E-4 5.660500333241741E-5 0.0 4.910374872295069 3.19413947375784E-4 56-57 3.9623502332692187E-4 6.064821785616151E-5 0.0 5.465055386378475 3.4367323451824853E-4 58-59 3.9623502332692187E-4 7.277786142739381E-5 0.0 6.043425094356497 3.5175966356573675E-4 60-61 4.124078814218983E-4 7.277786142739381E-5 0.0 6.6383760250963935 3.679325216607132E-4 62-63 4.1645109594564236E-4 7.68210759511379E-5 0.0 7.214744341885163 4.043214523744101E-4 64-65 4.285807395168747E-4 8.086429047488201E-5 0.0 7.771050228207114 4.2049431046938646E-4 66-67 4.366671685643629E-4 8.895071952237021E-5 0.0 8.358759761734985 4.2049431046938646E-4 68-69 4.5284002665933927E-4 8.895071952237021E-5 0.0 8.984536160003907 4.285807395168747E-4 70-71 4.770993138018039E-4 8.895071952237021E-5 0.0 9.630298167663696 4.487968121355952E-4 72-73 5.013586009442684E-4 8.895071952237021E-5 0.0 10.260089477954697 4.730560992780598E-4 74-75 5.175314590392449E-4 9.703714856985841E-5 0.0 10.864008258508257 4.770993138018039E-4 76-77 5.175314590392449E-4 9.703714856985841E-5 0.0 11.479624058679011 4.770993138018039E-4 78-79 5.215746735629889E-4 9.703714856985841E-5 0.0 12.124755324873096 4.770993138018039E-4 80-81 5.660500333241741E-4 1.0512357761734662E-4 0.0 12.791081121600648 4.851857428492921E-4 82-83 5.822228914191505E-4 1.0512357761734662E-4 0.0 13.456129262538695 4.932721718967803E-4 84-85 5.983957495141269E-4 1.0916679214109071E-4 0.0 14.096861511330946 4.932721718967803E-4 86-87 6.105253930853592E-4 1.253396502360671E-4 0.0 14.728565262091678 4.973153864205244E-4 88-89 6.145686076091033E-4 1.3746929380729943E-4 0.0 15.387241296940307 5.013586009442684E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10455 0.0 50.67308 1 GTATCAA 19735 0.0 45.118942 1 ATCAACG 24685 0.0 37.129147 3 TCAACGC 24750 0.0 37.107525 4 CAACGCA 25485 0.0 36.016644 5 AACGCAG 26330 0.0 35.046352 6 TATCAAC 25725 0.0 34.151497 2 ACGCAGA 31000 0.0 29.522385 7 CGCAGAG 32050 0.0 28.48018 8 GTACATG 39000 0.0 24.110579 1 GCAGAGT 37870 0.0 23.514017 9 ACATGGG 41365 0.0 22.582207 3 TACATGG 40715 0.0 22.569933 2 CATGGGG 32770 0.0 20.283674 4 AGAGTAC 33980 0.0 19.827387 10-11 CAGAGTA 35470 0.0 19.476553 10-11 GAGTACT 14765 0.0 17.179043 12-13 TCGCCGT 7770 0.0 17.129423 94-95 TAGGACG 6520 0.0 16.979061 4 ATGGGGA 19895 0.0 16.620575 5 >>END_MODULE