FastQCFastQC Report
Fri 27 May 2016
SRR940195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12366398
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111200.052.044061
GTATCAA212100.046.484281
TCAACGC258150.037.3915184
ATCAACG257900.037.3909263
CAACGCA268500.036.0026635
TATCAAC272700.035.658352
AACGCAG280300.034.5521666
ACGCAGA326050.029.7035857
CGCAGAG338100.028.4621948
GCAGAGT405750.023.4709119
GTACATG397850.023.3602871
TACATGG415800.021.9353872
ACATGGG419250.021.323973
AGAGTAC361900.020.11998610-11
CAGAGTA376400.019.73609510-11
CATGGGG325950.019.018714
GAGTACT160500.017.80060212-13
GTACTTT174400.016.47758514-15
GTATATA53650.015.8530181
ATGGGGA198500.015.8420725