FastQCFastQC Report
Fri 27 May 2016
SRR940194_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940194_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12586559
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106350.051.0925831
GTATCAA196850.045.9891361
ATCAACG250850.037.54113
TCAACGC253150.037.2555584
CAACGCA263650.035.7885675
TATCAAC257050.034.787922
AACGCAG274050.034.586096
ACGCAGA317950.029.6329127
CGCAGAG329200.028.4765558
GTACATG396250.024.1306481
GCAGAGT392050.023.486649
ACATGGG424650.023.1045513
TACATGG416600.022.6745722
CATGGGG334050.020.9117764
AGAGTAC348950.019.89668810-11
CAGAGTA364300.019.62538710-11
GAGTACT155000.017.4035712-13
TCGCCGT79400.016.93769894-95
ATGGGGA199650.016.8874175
GTACTTT164950.016.42763514-15
Produced by FastQC (version 0.11.5)