FastQCFastQC Report
Fri 27 May 2016
SRR940194_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940194_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12586559
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118550.051.1414261
GTATCAA216000.045.2925031
TCAACGC262400.036.7881244
ATCAACG263800.036.4308133
CAACGCA272000.035.4519735
TATCAAC275650.035.0723272
AACGCAG283750.034.117716
ACGCAGA333300.029.015837
CGCAGAG346650.027.9797178
GTACATG408900.023.937231
GCAGAGT413750.023.235469
TACATGG426200.022.5273972
ACATGGG424850.021.8157143
CATGGGG332600.019.8822154
AGAGTAC372750.019.75754210-11
CAGAGTA390500.019.5223810-11
GAGTACT164750.017.8319612-13
ATGGGGA198600.016.7190365
AGTACTT181050.016.39707212-13
GTACTTT179650.016.28715514-15
Produced by FastQC (version 0.11.5)