FastQCFastQC Report
Fri 27 May 2016
SRR940193_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940193_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11745170
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100800.050.803841
GTATCAA186850.045.0768931
TCAACGC233050.035.953074
ATCAACG233500.035.86393
CAACGCA239800.035.0198335
TATCAAC244250.033.8393142
AACGCAG250250.033.824926
ACGCAGA294250.028.5731497
CGCAGAG304050.027.5576348
GTACATG373850.025.4392931
TACATGG393150.023.7174972
ACATGGG396800.023.330943
GCAGAGT361050.022.7860439
CATGGGG307250.021.0248814
AGAGTAC323950.019.71366910-11
CAGAGTA338050.018.96167210-11
TCGCCGT76950.017.8813994-95
ATGGGGA185950.017.0121445
GAGTACT140450.016.69086312-13
TAGGACG60400.016.3573474
Produced by FastQC (version 0.11.5)