##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940193_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11745170 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.970418222980168 33.0 31.0 34.0 30.0 34.0 2 31.588296125130586 34.0 31.0 34.0 28.0 34.0 3 31.995344809823955 34.0 31.0 34.0 30.0 34.0 4 35.51876413879067 37.0 35.0 37.0 33.0 37.0 5 35.58768676826304 37.0 35.0 37.0 35.0 37.0 6 35.667795613005175 37.0 37.0 37.0 35.0 37.0 7 35.645122037399204 37.0 37.0 37.0 35.0 37.0 8 35.643622527387855 37.0 37.0 37.0 35.0 37.0 9 37.371269551654 39.0 38.0 39.0 35.0 39.0 10-11 37.30411271186368 39.0 38.0 39.0 35.0 39.0 12-13 37.214733247794626 39.0 38.0 39.0 35.0 39.0 14-15 38.605142667155945 41.0 39.0 41.0 35.0 41.0 16-17 38.53348504108497 41.0 39.0 41.0 35.0 41.0 18-19 38.45725319429178 41.0 39.0 41.0 34.0 41.0 20-21 38.37944337970417 40.0 39.0 41.0 34.0 41.0 22-23 38.267300005023344 40.0 38.0 41.0 34.0 41.0 24-25 38.149340282005284 40.0 38.0 41.0 34.0 41.0 26-27 37.98116898265414 40.0 38.0 41.0 33.5 41.0 28-29 37.81634744324688 40.0 38.0 41.0 33.0 41.0 30-31 37.65915133625141 40.0 38.0 41.0 33.0 41.0 32-33 37.717132319072434 40.0 38.0 41.0 33.0 41.0 34-35 37.79170612260189 40.0 38.0 41.0 33.0 41.0 36-37 37.9126111414309 40.0 38.0 41.0 33.0 41.0 38-39 37.82514382507874 40.0 38.0 41.0 33.0 41.0 40-41 37.68298555917028 40.0 38.0 41.0 33.0 41.0 42-43 37.54755827289005 40.0 38.0 41.0 33.0 41.0 44-45 37.39767947164664 40.0 38.0 41.0 32.0 41.0 46-47 37.183933438170754 40.0 37.5 41.0 31.5 41.0 48-49 36.999848022633984 40.0 37.0 41.0 31.0 41.0 50-51 36.080559625786606 39.0 36.0 40.0 30.0 40.5 52-53 36.093247777597085 39.0 36.0 40.0 30.0 41.0 54-55 36.50155979010947 40.0 36.0 41.0 30.5 41.0 56-57 36.39718339538721 40.0 36.0 41.0 30.0 41.0 58-59 36.14869171753155 39.0 35.0 41.0 30.0 41.0 60-61 35.854186699724224 39.0 35.0 41.0 29.5 41.0 62-63 35.51993943042119 38.5 35.0 40.5 29.0 41.0 64-65 35.14462357718109 37.5 35.0 40.0 28.0 41.0 66-67 34.74235392080319 37.0 35.0 40.0 28.0 41.0 68-69 34.290950450270195 36.5 34.0 39.0 27.5 41.0 70-71 33.84514357816873 36.0 34.0 39.0 26.0 41.0 72-73 33.39758113335098 35.5 34.0 38.5 26.0 40.0 74-75 32.93828441819063 35.0 33.5 37.0 25.5 39.5 76-77 32.39259955368888 35.0 33.0 37.0 24.0 39.0 78-79 31.92443438451721 35.0 33.0 36.0 22.0 39.0 80-81 31.532686159502163 35.0 32.5 36.0 20.5 37.0 82-83 31.12577885207281 35.0 32.0 35.0 19.0 37.0 84-85 30.781014451046687 35.0 32.0 35.0 17.5 36.5 86-87 30.42077019745138 35.0 31.0 35.0 11.0 36.0 88-89 30.05275794220092 34.0 31.0 35.0 4.5 36.0 90-91 29.67581410060476 34.0 31.0 35.0 2.0 35.5 92-93 29.294276328056554 34.0 30.0 35.0 2.0 35.0 94-95 28.843457906526684 34.0 30.0 35.0 2.0 35.0 96-97 28.349818563715978 34.0 29.0 35.0 2.0 35.0 98-99 27.79806154359622 34.0 29.0 35.0 2.0 35.0 100-101 25.83845631863992 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.10469653858193695 1101 2 -0.029162516235096803 1101 3 0.06868383064674788 1101 4 0.08762357194248693 1101 5 0.21442651640518307 1101 6 0.3385062605262732 1101 7 0.32040217110582603 1101 8 0.34908994404084837 1101 9 0.46040126916999213 1101 10-11 0.5245139367881677 1101 12-13 0.5279723074533251 1101 14-15 0.6023507135547916 1101 16-17 0.6164862590997942 1101 18-19 0.6521056670217362 1101 20-21 0.6557759614568042 1101 22-23 0.6688527615823148 1101 24-25 0.6623945959642867 1101 26-27 0.7408590627382097 1101 28-29 0.734042243006833 1101 30-31 0.7456996090650705 1101 32-33 0.7303171208330141 1101 34-35 0.7265844532924746 1101 36-37 0.7430283857830986 1101 38-39 0.8521685082582593 1101 40-41 0.8322217384139847 1101 42-43 0.8362328349406241 1101 44-45 0.8616571216299675 1101 46-47 0.9427074264970017 1101 48-49 0.9087873422120794 1101 50-51 0.8295756005602541 1101 52-53 0.9458463597580931 1101 54-55 0.9585459151688838 1101 56-57 0.96712376599843 1101 58-59 0.9931250686977577 1101 60-61 0.9985075221591231 1101 62-63 1.0120980008095657 1101 64-65 0.9788843119896882 1101 66-67 0.9324496082399634 1101 68-69 0.9219135758240711 1101 70-71 0.8498631518373827 1101 72-73 0.8435829325971227 1101 74-75 0.7834418234387144 1101 76-77 0.9220733023133789 1101 78-79 0.9158022743667331 1101 80-81 0.840919543596069 1101 82-83 0.795866248428581 1101 84-85 0.7845884109547576 1101 86-87 0.7180797490232642 1101 88-89 0.6823010727921464 1101 90-91 0.6802301687827033 1101 92-93 0.622646390570722 1101 94-95 0.7295323999961809 1101 96-97 0.5387444755511837 1101 98-99 0.5008965416152193 1101 100-101 0.7329259390389247 1102 1 0.3512322906264451 1102 2 0.7950065547051643 1102 3 0.44869785396315365 1102 4 0.35299455401360547 1102 5 0.3610204223447795 1102 6 0.2759341704097267 1102 7 0.26397832557100287 1102 8 0.25075002731762197 1102 9 0.23434053092272222 1102 10-11 0.26999777023593197 1102 12-13 0.3287789302274362 1102 14-15 0.36382748069896564 1102 16-17 0.4146855617472127 1102 18-19 0.39723019262192594 1102 20-21 0.3945598460249897 1102 22-23 0.36130283805703556 1102 24-25 0.4274787875432011 1102 26-27 0.4286622530241715 1102 28-29 0.37463449875450294 1102 30-31 0.4497797336186764 1102 32-33 0.42198562308237086 1102 34-35 0.4139648573076258 1102 36-37 0.4589842670641602 1102 38-39 0.44943959184544013 1102 40-41 0.43335718168527393 1102 42-43 0.34415616416504946 1102 44-45 0.4333340107317767 1102 46-47 0.49998153390554734 1102 48-49 0.4648194406337325 1102 50-51 0.4433857937743326 1102 52-53 0.5566221278593702 1102 54-55 0.5999930809427312 1102 56-57 0.5862520169975909 1102 58-59 0.5277028498824592 1102 60-61 0.4928067201608215 1102 62-63 0.5482539713104444 1102 64-65 0.533332728759973 1102 66-67 0.46701599295783325 1102 68-69 0.5607380151364296 1102 70-71 0.5359374353053781 1102 72-73 0.48000816856266937 1102 74-75 0.45236077992014856 1102 76-77 0.5472706042889044 1102 78-79 0.5156601139952386 1102 80-81 0.44285293514917967 1102 82-83 0.3878854344783029 1102 84-85 0.2487206876760446 1102 86-87 0.3233092417452177 1102 88-89 0.20277447028830764 1102 90-91 0.15740423992021135 1102 92-93 0.10786097276080397 1102 94-95 0.08358112301126042 1102 96-97 -0.06995937987663581 1102 98-99 -0.10351780002808297 1102 100-101 0.13407960018529508 1103 1 0.2308701195882925 1103 2 0.6844756148517526 1103 3 0.3113082414252304 1103 4 0.2525921712581578 1103 5 0.16251490556426518 1103 6 0.17234720074633003 1103 7 0.15658758241050919 1103 8 0.0894306742462021 1103 9 0.13897416115197814 1103 10-11 0.13444892885848958 1103 12-13 0.15868808331271822 1103 14-15 0.11804708048386914 1103 16-17 0.09042601203345413 1103 18-19 0.11021121940933654 1103 20-21 0.11360080112029891 1103 22-23 0.13523732113488052 1103 24-25 0.06409893807028766 1103 26-27 0.12999473725236044 1103 28-29 0.021552862999108413 1103 30-31 0.09171592856532129 1103 32-33 0.0923102141991663 1103 34-35 0.05914922087335128 1103 36-37 0.10406084840548857 1103 38-39 0.15664394668994674 1103 40-41 0.08319002912913476 1103 42-43 0.12102792627194248 1103 44-45 0.15526061435675587 1103 46-47 0.09439022588905033 1103 48-49 0.10706845380511254 1103 50-51 0.004566133386035176 1103 52-53 0.20744069733600412 1103 54-55 0.25295060115103496 1103 56-57 0.24907766538560594 1103 58-59 0.24212213228365442 1103 60-61 0.21414180203863964 1103 62-63 0.0653898589908053 1103 64-65 0.19888211521385557 1103 66-67 0.20286196422333092 1103 68-69 0.1769355050531516 1103 70-71 0.09978251573134855 1103 72-73 0.11134224755043931 1103 74-75 0.1894427214748049 1103 76-77 0.28997252412143837 1103 78-79 0.34265781910964677 1103 80-81 0.1794004235735791 1103 82-83 0.09838162096678005 1103 84-85 0.0217239247471106 1103 86-87 0.10003705182288414 1103 88-89 0.08062701585846455 1103 90-91 0.0808843303396678 1103 92-93 0.06303912296110425 1103 94-95 -0.09764392172041525 1103 96-97 -0.27712285398891723 1103 98-99 -0.3268806673982141 1103 100-101 -0.26636772292032873 1104 1 0.15932311135432187 1104 2 0.6639963466569689 1104 3 0.33944154101914137 1104 4 0.14990939660460612 1104 5 0.08205256812485828 1104 6 0.07131222901605128 1104 7 0.0748352298068582 1104 8 -0.04629061144795088 1104 9 0.06985961949580854 1104 10-11 0.01894854762515763 1104 12-13 0.06320240573659675 1104 14-15 0.018438265466571124 1104 16-17 0.0674716636612942 1104 18-19 0.07207845254322365 1104 20-21 0.04048196841570473 1104 22-23 0.010918907509477549 1104 24-25 -0.01224729097528865 1104 26-27 0.015792949395667222 1104 28-29 -0.002941979714854881 1104 30-31 0.01569913987069782 1104 32-33 -0.00562055449375265 1104 34-35 -0.013758740410651171 1104 36-37 0.05702311093005363 1104 38-39 0.11175638092673523 1104 40-41 0.02306700322782973 1104 42-43 -0.06030010771461036 1104 44-45 0.009459674758097947 1104 46-47 0.027430017881037827 1104 48-49 0.07035136944655562 1104 50-51 -0.032620625442540074 1104 52-53 0.1333442027589129 1104 54-55 0.18065523059260613 1104 56-57 0.19512660549775518 1104 58-59 0.17333201353345373 1104 60-61 0.22287178636386074 1104 62-63 0.18712317859900196 1104 64-65 0.22264667690281215 1104 66-67 0.18816485049815412 1104 68-69 0.20915002214464806 1104 70-71 0.12720953479745134 1104 72-73 -0.03298118167020192 1104 74-75 -0.02975668667941278 1104 76-77 0.10999441405469668 1104 78-79 0.04418072233557524 1104 80-81 -0.16046055944239512 1104 82-83 -0.26491434465599895 1104 84-85 -0.20117556006071524 1104 86-87 -0.19744434802299438 1104 88-89 -0.2725477921098083 1104 90-91 -0.3375454017537436 1104 92-93 -0.3557981782694597 1104 94-95 -0.41541806566568695 1104 96-97 -0.5372997815849203 1104 98-99 -0.5778511846140191 1104 100-101 -0.37938153240872907 1105 1 -0.030180369975472132 1105 2 0.515704621464387 1105 3 0.1524898701219044 1105 4 0.12817602469941392 1105 5 0.02870814695883439 1105 6 0.03657973732624953 1105 7 -0.0016792734517636632 1105 8 -0.044991650395957095 1105 9 0.007017418352511129 1105 10-11 0.001283988034288086 1105 12-13 -0.09046189252224934 1105 14-15 -0.2642752876437484 1105 16-17 -0.1615490656494316 1105 18-19 -0.2899589944003651 1105 20-21 -0.1691091623456913 1105 22-23 -0.21463424514151086 1105 24-25 -0.07985277745147101 1105 26-27 -0.0490755926812767 1105 28-29 0.08954213556921076 1105 30-31 -0.040686788815136765 1105 32-33 0.09238871774538637 1105 34-35 -0.00754602831442952 1105 36-37 -0.012274115800302354 1105 38-39 -0.20136860817253677 1105 40-41 -0.2696112264943977 1105 42-43 -0.1483180420977135 1105 44-45 -0.12642411247287555 1105 46-47 -0.02785744801724377 1105 48-49 -0.2703270483878697 1105 50-51 -0.22829764760833626 1105 52-53 0.03899723679046474 1105 54-55 -0.11848369196804498 1105 56-57 0.03192045489932838 1105 58-59 0.004394033453600343 1105 60-61 0.025431922285356734 1105 62-63 0.05492831381526031 1105 64-65 0.10067435430126181 1105 66-67 -0.025150693449589312 1105 68-69 -0.08779268841131938 1105 70-71 -0.22793318071294522 1105 72-73 -0.38471125417601826 1105 74-75 -0.37118348589951466 1105 76-77 -0.26760758854537414 1105 78-79 -0.19767955363650458 1105 80-81 -0.23208852122344226 1105 82-83 -0.39033459444140206 1105 84-85 -0.4700138115391823 1105 86-87 -0.35672868932411816 1105 88-89 -0.3693262755611606 1105 90-91 -0.5658052659521289 1105 92-93 -0.5015982319930501 1105 94-95 -0.6975935453701609 1105 96-97 -0.6705132268259248 1105 98-99 -0.7217532837443912 1105 100-101 -0.6663632581407732 1106 1 0.040274365440822635 1106 2 0.57220492897385 1106 3 0.11468971803503081 1106 4 0.06964115112034364 1106 5 0.019758067627911657 1106 6 0.0028633568581213353 1106 7 0.04958758457036794 1106 8 -0.01870163012988968 1106 9 -0.012641612016658144 1106 10-11 -0.13075054499964267 1106 12-13 -0.04981110925967158 1106 14-15 -0.045329219661773834 1106 16-17 0.02488204952575046 1106 18-19 -0.013389731360980761 1106 20-21 -0.022032998958749772 1106 22-23 -0.09361873068447579 1106 24-25 -0.06376224629607208 1106 26-27 -0.2610518066478704 1106 28-29 -0.16178207223028807 1106 30-31 -0.12427545791351946 1106 32-33 -0.14913647073991854 1106 34-35 -0.19469100997247324 1106 36-37 -0.19192706789849723 1106 38-39 -0.07748859779056971 1106 40-41 -0.20341955064133543 1106 42-43 -0.1632055376609216 1106 44-45 -0.09872162727540967 1106 46-47 -0.09280847365932487 1106 48-49 -0.09034935272759981 1106 50-51 -0.16408112477986236 1106 52-53 0.014656690162389907 1106 54-55 0.10867430179732906 1106 56-57 0.05249284480876071 1106 58-59 0.0788888778571959 1106 60-61 0.03477686923547907 1106 62-63 0.009216268533357663 1106 64-65 -0.059368459488858605 1106 66-67 0.053726475133856866 1106 68-69 -0.21749049958766875 1106 70-71 -0.05598711793481925 1106 72-73 -0.10400437576064547 1106 74-75 -0.02617275939280006 1106 76-77 -3.821176694032147E-4 1106 78-79 0.11311200704194135 1106 80-81 0.05445509502649415 1106 82-83 -0.05871637151546949 1106 84-85 -0.16255275438194516 1106 86-87 -0.19788042929094374 1106 88-89 -0.20549340064890842 1106 90-91 -0.1313366157653526 1106 92-93 -0.2518995838156002 1106 94-95 -0.24908992090399096 1106 96-97 -0.23752726795054002 1106 98-99 -0.3431616010912357 1106 100-101 -0.35359672350277194 1107 1 0.09257295343001815 1107 2 0.5410712730346283 1107 3 0.18532134906275388 1107 4 0.07108374805567053 1107 5 0.09872431517617741 1107 6 0.05645489270279569 1107 7 0.06228819301146871 1107 8 0.012265253542665278 1107 9 0.002375464891272827 1107 10-11 0.031149102186972755 1107 12-13 0.016799707209223413 1107 14-15 -0.027442515416652213 1107 16-17 0.009769238436987848 1107 18-19 0.0465261714937526 1107 20-21 0.04552432585253996 1107 22-23 0.020697221141730893 1107 24-25 0.050602277321729616 1107 26-27 0.07630156291762091 1107 28-29 0.01943402322873311 1107 30-31 -0.029191095716470272 1107 32-33 0.007171551409996368 1107 34-35 -0.042256109636156225 1107 36-37 0.00726444001056592 1107 38-39 0.029690203620013733 1107 40-41 0.06944665626566149 1107 42-43 0.07415220736262285 1107 44-45 0.010999420444846919 1107 46-47 -0.07751323962700951 1107 48-49 0.07952654413611526 1107 50-51 -0.17353112549507443 1107 52-53 0.06364467616428016 1107 54-55 0.11309685913476386 1107 56-57 0.11590894582126765 1107 58-59 0.143491001468945 1107 60-61 0.01947696524312903 1107 62-63 0.0606079376612243 1107 64-65 0.05400838861627477 1107 66-67 0.02350642745820153 1107 68-69 0.13953305720535525 1107 70-71 0.11255100366420834 1107 72-73 0.08298080321662837 1107 74-75 0.06500766346156439 1107 76-77 0.22647511702427892 1107 78-79 0.1782414154211125 1107 80-81 0.11497102463883735 1107 82-83 0.051900193632157254 1107 84-85 -0.06918100536831773 1107 86-87 -0.01681040681623358 1107 88-89 -0.03466632548274973 1107 90-91 0.032538655912730974 1107 92-93 -0.008452863011054745 1107 94-95 -0.027543019780086553 1107 96-97 -0.05228817020364929 1107 98-99 -0.06457008628241567 1107 100-101 0.12913412187779372 1108 1 0.12458392496967363 1108 2 0.2953356182664031 1108 3 0.1238619395755407 1108 4 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2316 100-101 -1.4154984854064132 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104250.0 3 30224.0 4 23607.0 5 18476.0 6 16507.0 7 15891.0 8 16837.0 9 19021.0 10 21565.0 11 24735.0 12 27375.0 13 30182.0 14 33105.0 15 37688.0 16 44309.0 17 52995.0 18 49827.0 19 43723.0 20 46144.0 21 49203.0 22 53137.0 23 59096.0 24 67973.0 25 77951.0 26 90722.0 27 106735.0 28 126991.0 29 152176.0 30 182852.0 31 227444.0 32 287768.0 33 379548.0 34 535475.0 35 818018.0 36 1476971.0 37 2842127.0 38 3063635.0 39 490841.0 40 46.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.51672491105782 18.730486610149182 14.118012876761352 24.634775602031645 2 15.97726558057754 20.429506469826496 39.04267145355356 24.550556496042404 3 18.274242365657738 25.023416712557573 29.614787200464942 27.087553721319747 4 13.41196479000385 17.17497351415247 36.92630461960872 32.48675707623496 5 14.831451889757066 36.55608063356379 34.00639535532359 14.60607212135556 6 32.01595206409169 35.48058463194102 18.37860823640369 14.124855067563596 7 28.64705161443018 31.512668058118003 21.959772935717893 17.88050739173392 8 26.609471886235443 34.20932145015476 20.663065080637963 18.518141582971833 9 27.092143119400763 16.192390695486182 19.87528617654341 36.84018000856965 10-11 25.327492063459623 25.60272850336428 27.857988011490598 21.2117914216855 12-13 26.422575394497738 22.814421323476967 27.722977051372265 23.04002623065303 14-15 23.43215720071808 24.24329611222199 25.052923412851165 27.271623274208768 16-17 23.580297433849353 27.14236441644861 25.82986817205018 23.447469977651856 18-19 23.857631555949492 26.33842960613835 26.925131006981644 22.87880783093051 20-21 24.43098610420858 25.78617809367999 26.551894894393662 23.23094090771777 22-23 24.311811916784393 25.740609111093615 26.428319168363462 23.519259803758533 24-25 24.14594549716118 25.88787342704535 26.397179698426992 23.569001377366483 26-27 23.831577778405137 26.179278273329537 26.241599972952486 23.74754397531284 28-29 23.811160222354317 26.116910308766343 26.252503840901202 23.819425627978138 30-31 23.944143390630952 25.818518310496568 26.274795138083228 23.962543160789252 32-33 23.758196663018833 25.978755513379326 26.37711305929176 23.885934764310086 34-35 24.0723354594557 25.97921068643931 26.201024347632934 23.747429506472056 36-37 23.99113101490917 25.73502095071391 26.196709139294356 24.077138895082566 38-39 23.875830779022618 25.74291311835055 26.449855546682105 23.931400555944727 40-41 24.018535408436854 25.41272740131174 26.690350336046137 23.878386854205267 42-43 23.734825928475843 25.57469553928813 26.579906593586728 24.110571938649297 44-45 23.544241080037835 25.687066500358252 26.533172554717222 24.23551986488669 46-47 23.709960092458342 25.630313875431604 26.49569188705613 24.164034145053932 48-49 23.59631061508881 25.731512156272572 26.390263979325983 24.281913249312634 50-51 23.290630908788586 25.881650604023104 26.51888055222536 24.308837934962956 52-53 23.492912780807185 25.71005584429703 26.393566261410385 24.4034651134854 54-55 23.256533909832914 25.559694755464047 26.63752713839294 24.5462441963101 56-57 23.27107915485192 25.779275204582923 26.559199824687617 24.39044581587754 58-59 23.50288128594315 25.52052027563152 26.359021476346534 24.6175769620788 60-61 23.35382045561324 25.302319227522887 26.696038577695113 24.64782173916876 62-63 23.377483610103518 25.326512902629194 26.662029639131628 24.63397384813566 64-65 23.32403700091334 25.32984151663818 26.462230533827398 24.883890948621083 66-67 23.299374975276088 25.59313646776954 26.208069364999698 24.899419191954678 68-69 23.235493510390516 25.303138775418265 26.534540317394324 24.926827396796895 70-71 23.252516669238105 25.203771329817528 26.416956036767562 25.126755964176805 72-73 23.20067971743443 25.231163111320974 26.378486457809537 25.189670713435063 74-75 23.058087484507126 25.343008555418645 26.48103844881795 25.117865511256277 76-77 23.24690714347834 25.081479726171217 26.583808892595485 25.087804237754955 78-79 23.11431041085231 25.07042594180206 26.64527157320493 25.1699920741407 80-81 23.155906331801557 25.112617959352228 26.734872696324054 24.99660301252216 82-83 23.127027633190966 25.03494192144437 26.787009470162392 25.051020975202277 84-85 23.15821840513076 24.922460243495998 26.791100456896714 25.12822089447652 86-87 23.262193774460425 24.875307563605727 26.820696070313684 25.041802591620165 88-89 23.295614974072663 24.638520621513443 26.83234720790023 25.23351719651366 90-91 23.45104880023337 24.656821298750426 26.922011283710845 24.97011861730536 92-93 23.53795685654251 24.53494769960897 26.879309214084522 25.047786229763997 94-95 23.502654142287415 24.379776758527687 26.898230933916 25.219338165268894 96-97 23.709710665193207 24.208976022873223 26.76886558324925 25.31244772868432 98-99 23.654041311126438 24.042645101293683 26.819950077556726 25.483363510023153 100-101 23.65133694651498 23.95136554339005 27.07699291076991 25.320304599325063 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 853.0 1 517.5 2 263.5 3 373.5 4 330.0 5 333.5 6 523.0 7 995.0 8 1782.0 9 2372.0 10 2336.0 11 2238.5 12 2463.5 13 2716.0 14 3008.5 15 3284.5 16 3552.0 17 3934.5 18 4301.5 19 4711.5 20 5356.5 21 6220.5 22 7406.0 23 9014.0 24 11124.5 25 14199.5 26 18592.5 27 24597.0 28 31926.0 29 41864.0 30 56002.5 31 74185.5 32 95411.5 33 118730.0 34 146799.0 35 175471.0 36 204043.5 37 232504.5 38 255725.0 39 272227.5 40 290318.0 41 320536.0 42 353306.5 43 386019.5 44 417818.5 45 442586.5 46 463444.0 47 486602.5 48 514716.5 49 532459.5 50 540287.0 51 542369.5 52 532753.5 53 522471.0 54 508955.5 55 477905.0 56 437999.0 57 393891.5 58 348862.5 59 303606.0 60 251644.0 61 200279.0 62 157501.5 63 122514.5 64 92694.0 65 69476.0 66 51747.5 67 39480.0 68 28765.5 69 19942.0 70 14450.5 71 10253.5 72 7371.0 73 5370.0 74 4035.5 75 3101.0 76 2177.0 77 1420.5 78 938.5 79 659.5 80 487.5 81 366.5 82 285.0 83 232.5 84 192.0 85 166.5 86 131.0 87 110.5 88 101.0 89 81.0 90 76.5 91 67.0 92 48.5 93 45.0 94 39.5 95 38.5 96 33.5 97 25.0 98 24.5 99 17.5 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030250732854441444 2 0.035810465067768284 3 0.03027627526889777 4 0.025968121363930875 5 0.027338897606420342 6 0.029731370427162826 7 0.029569601802272763 8 0.02814774073087065 9 0.031749221169212534 10-11 0.01852250754991201 12-13 0.02709624466908525 14-15 0.02324359715525616 16-17 0.03724509734639856 18-19 0.04219606868184965 20-21 0.04529947203829319 22-23 0.03555078385412898 24-25 0.014857171075429306 26-27 0.02444834770377951 28-29 0.029454660937219296 30-31 0.027036645702020492 32-33 0.033707472944197486 34-35 0.03604886093602732 36-37 0.03238778153062068 38-39 0.01937817843419891 40-41 0.01631734576851591 42-43 0.01587886765368232 44-45 0.023239340086180105 46-47 0.018318168234261405 48-49 0.02135345848548808 50-51 0.024371720460410536 52-53 0.02984631129221629 54-55 0.029816511808683912 56-57 0.032719832918552905 58-59 0.030859493732317198 60-61 0.03036567371949491 62-63 0.03551672730152054 64-65 0.03083820838693693 66-67 0.025495586696488857 68-69 0.02468674357203855 70-71 0.03411189450642264 72-73 0.017496553902582933 74-75 0.02621928843941807 76-77 0.04370307113477285 78-79 0.027462352609625915 80-81 0.02680676397191356 82-83 0.027121787083541574 84-85 0.026640738277947443 86-87 0.021089520202772716 88-89 0.024124810453999387 90-91 0.021204461067826176 92-93 0.03695135958015082 94-95 0.0709014854616834 96-97 0.08111419417513753 98-99 0.08145050263214582 100-101 0.08192303729958783 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.174517E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.409912815628616 #Duplication Level Percentage of deduplicated Percentage of total 1 76.3054596837184 36.17635190961553 2 12.167298101290621 11.537010843679038 3 4.0149104733116125 5.710396665067734 4 1.954594584109796 3.706686352901812 5 1.2206088799068844 2.8934480289183746 6 0.7980196449862987 2.2700425076375588 7 0.5784249451675331 1.9196153354544256 8 0.4081859264282057 1.5481647347622263 9 0.3359173777372558 1.433323223260009 >10 1.9688264662922377 17.341927255271813 >50 0.15595028667253943 5.090849476922276 >100 0.0850998216366283 7.42557882788278 >500 0.004990970212570016 1.584059509644415 >1k 0.0016767792489374336 1.222762554429651 >5k 3.605928065813706E-5 0.13978277455235183 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.514138152108484E-6 2 0.0 0.0 0.0 0.0 8.514138152108484E-6 3 0.0 0.0 0.0 0.0 8.514138152108484E-6 4 0.0 8.514138152108484E-6 0.0 5.959896706475939E-5 8.514138152108484E-6 5 0.0 8.514138152108484E-6 0.0 5.959896706475939E-5 8.514138152108484E-6 6 0.0 8.514138152108484E-6 0.0 6.811310521686787E-5 2.554241445632545E-5 7 0.0 8.514138152108484E-6 0.0 7.662724336897635E-5 2.554241445632545E-5 8 0.0 8.514138152108484E-6 0.0 7.662724336897635E-5 2.554241445632545E-5 9 0.0 8.514138152108484E-6 0.0 1.1919793412951878E-4 3.4056552608433934E-5 10-11 0.0 8.514138152108484E-6 0.0 2.1711052287876634E-4 3.4056552608433934E-5 12-13 4.257069076054242E-6 8.514138152108484E-6 0.0 3.065089734759054E-4 5.10848289126509E-5 14-15 8.514138152108484E-6 8.514138152108484E-6 0.0 4.3847811483358693E-4 6.385603614081363E-5 16-17 8.514138152108484E-6 8.514138152108484E-6 0.0 6.343032923320821E-4 6.811310521686787E-5 18-19 2.1285345380271207E-5 8.514138152108484E-6 0.0 7.492441573855466E-4 6.811310521686787E-5 20-21 3.4056552608433934E-5 8.514138152108484E-6 0.0 9.833829565685298E-4 7.237017429292211E-5 22-23 3.4056552608433934E-5 1.7028276304216967E-5 0.0 0.001494231245695039 8.514138152108483E-5 24-25 5.10848289126509E-5 1.7028276304216967E-5 0.0 0.0026478969653057384 1.021696578253018E-4 26-27 5.10848289126509E-5 1.7028276304216967E-5 0.0 0.0050999687531129816 1.021696578253018E-4 28-29 8.08843124450306E-5 1.7028276304216967E-5 0.0 0.019927340345009904 1.021696578253018E-4 30-31 8.514138152108484E-5 1.7028276304216967E-5 0.0 0.07795970598978133 1.1919793412951878E-4 32-33 8.514138152108484E-5 1.7028276304216967E-5 0.0 0.20372204063457575 1.2771207228162726E-4 34-35 1.0216965782530182E-4 2.1285345380271207E-5 0.0 0.3538731240160849 1.4474034858584422E-4 36-37 1.2771207228162726E-4 2.554241445632545E-5 0.0 0.5928692390148461 1.4474034858584422E-4 38-39 1.7879690119427817E-4 2.554241445632545E-5 0.0 0.9839193472721126 1.4899741766189844E-4 40-41 1.8305397027033242E-4 2.554241445632545E-5 0.0 1.4253561251135574 1.532544867379527E-4 42-43 2.043393156506036E-4 2.554241445632545E-5 0.0 1.8509225494394719 1.6176862489006118E-4 44-45 2.2136759195482057E-4 2.554241445632545E-5 0.0 2.3101581330878993 1.745398321182239E-4 46-47 2.7670948994352573E-4 2.554241445632545E-5 0.0 2.8235478924528126 2.0008224657454938E-4 48-49 3.2353724978012236E-4 2.554241445632545E-5 0.0 3.3530208587870587 2.554241445632545E-4 50-51 3.3205138793223086E-4 2.554241445632545E-5 0.0 3.893936826797739 2.554241445632545E-4 52-53 3.3205138793223086E-4 2.554241445632545E-5 0.0 4.404210411598981 2.7670948994352573E-4 54-55 3.4056552608433937E-4 3.4056552608433934E-5 0.0 4.915412037458802 2.8948069717168844E-4 56-57 4.0016449314909876E-4 3.4056552608433934E-5 0.0 5.471214976028444 2.8948069717168844E-4 58-59 4.086786313012072E-4 4.682775983659666E-5 0.0 6.052206992321099 2.8948069717168844E-4 60-61 4.086786313012072E-4 5.10848289126509E-5 0.0 6.647711357094023 2.9799483532379694E-4 62-63 4.1293570037726147E-4 5.534189798870515E-5 0.0 7.22627258694425 3.065089734759054E-4 64-65 4.171927694533157E-4 5.959896706475939E-5 0.0 7.785332183357074 3.065089734759054E-4 66-67 4.299639766814784E-4 5.959896706475939E-5 0.0 8.373986923986626 3.065089734759054E-4 68-69 4.342210457575327E-4 6.811310521686787E-5 0.0 9.002577229618643 3.065089734759054E-4 70-71 4.3847811483358693E-4 7.662724336897635E-5 0.0 9.643559863331054 3.1928018070406816E-4 72-73 4.5124932206174964E-4 7.662724336897635E-5 0.0 10.265245202921712 3.4482259516039357E-4 74-75 4.682775983659666E-4 9.365551967319332E-5 0.0 10.86509177815221 3.490796642364478E-4 76-77 4.682775983659666E-4 9.365551967319332E-5 0.0 11.478578002702388 3.490796642364478E-4 78-79 4.7253466744202085E-4 9.365551967319332E-5 0.0 12.120569561785825 3.490796642364478E-4 80-81 4.980770818983463E-4 9.791258874924756E-5 0.0 12.785813232162667 3.5759380238855633E-4 82-83 5.10848289126509E-4 1.1068379597741028E-4 0.0 13.45172951945353 3.9165035499699025E-4 84-85 5.10848289126509E-4 1.1068379597741028E-4 0.0 14.095551618239668 3.9165035499699025E-4 86-87 5.193624272786175E-4 1.1068379597741028E-4 0.0 14.72943346073322 4.0016449314909876E-4 88-89 5.193624272786175E-4 1.2771207228162726E-4 0.0 15.382071949575868 4.0016449314909876E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10080 0.0 50.80384 1 GTATCAA 18685 0.0 45.076893 1 TCAACGC 23305 0.0 35.95307 4 ATCAACG 23350 0.0 35.8639 3 CAACGCA 23980 0.0 35.019833 5 TATCAAC 24425 0.0 33.839314 2 AACGCAG 25025 0.0 33.82492 6 ACGCAGA 29425 0.0 28.573149 7 CGCAGAG 30405 0.0 27.557634 8 GTACATG 37385 0.0 25.439293 1 TACATGG 39315 0.0 23.717497 2 ACATGGG 39680 0.0 23.33094 3 GCAGAGT 36105 0.0 22.786043 9 CATGGGG 30725 0.0 21.024881 4 AGAGTAC 32395 0.0 19.713669 10-11 CAGAGTA 33805 0.0 18.961672 10-11 TCGCCGT 7695 0.0 17.88139 94-95 ATGGGGA 18595 0.0 17.012144 5 GAGTACT 14045 0.0 16.690863 12-13 TAGGACG 6040 0.0 16.357347 4 >>END_MODULE