FastQCFastQC Report
Fri 27 May 2016
SRR940192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11881754
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102400.052.5972251
GTATCAA196600.045.369271
ATCAACG235600.036.771253
TCAACGC238200.036.4293984
CAACGCA244350.035.6107525
TATCAAC247850.035.4342542
AACGCAG254300.034.4037486
ACGCAGA295450.029.5483477
CGCAGAG308950.028.1187068
GTACATG384900.025.6417311
TACATGG397950.024.3726392
ACATGGG401950.023.4792773
GCAGAGT367850.023.3493259
CATGGGG314800.020.896344
AGAGTAC326050.019.88169310-11
CAGAGTA342800.019.57544510-11
GAGTACT139850.017.42817112-13
TCGCCGT69650.017.16509294-95
ATGGGGA185600.016.8645765
TAGGACG63650.016.491084
Produced by FastQC (version 0.11.5)